ElectrophoresisofPCRproductswithSunrisegelapparatus
Electrophoresis of PCR products with Life Technologies Sunrise gel apparatusGel: In a 500 ml Pyrex? glass bottle, add:Agarose:3 gH2O270 mls10X TA30 mlsScrew cap partially on. Microwave at high power for 2 minutes. Carefully swirl (do little-by-little in case it boils violently) to mix in agarose.If agarose is not fully dissolved, microwave another 20 - 30 sec, repeating until dissolved. Cool until......閱讀全文
Electrophoresis－of－PCR－products－with－Sunrise－gel－apparatus
Electrophoresis of PCR products with Life Technologies Sunrise gel apparatusGel:?In a 500 ml Pyrex? glass bottle, add:Agarose:3 gH2O270 mls10X TA30 ml
Gene－splicing－and－mutagenesis－by－PCRdriven－overlap－extension
實驗概要? ? ? ? Extension of overlapping gene segments by PCR is a simple, versatile technique for site-directed mutagenesis and gene splicing.Initial
RNA－gel－electrophoresis
MaterialsDEPC H2ODEPC 0.1% (v/v)q.s. de-ioinized H2O37oC x1 hr, or r.t. overnightAutoclave.(NaOAc, EDTA and ethidium bromide solutions should also be
RNA－gel－electrophoresis
實驗概要RNA gel electrophoresis主要試劑DEPC H2ODEPC 0.1% (v/v)q.s. de-ioinized H2O37oC x1 hr, or r.t. overnightAutoclave.(NaOAc, EDTA and ethidium bromide sol
Agarose－Gel－Electrophoresis
實驗概要Separating nucleic acid fragments by agarose gel electrophoresis.實驗原理?Agarose ?gel electrophoresis remains the most widely used technique for ?sep
Gel－Electrophoresis－of－DNA
What is Electrophoresis?Electrophoresis is a technique used in the laboratory that results in the separation of charged molecules. In this CyberLab we
Agarose－gel－electrophoresis
General ProcedureCast a gelPlace it in gel box in running bufferLoad samplesRun the gelImage the gelCasting Gels0.7% agarose gel with 1kbp ladder in U
基于PCR技術的染色質沉淀分析2
METHOD?Analysis of precipitated chromatin fractions (from?Chromatin Immunoprecipitation on Unfixed Chromatin from Cells and Tissues to Analyze Histone
基于PCR技術的染色質沉淀分析
INTRODUCTION?After chromatin immunoprecipitation (ChIP), different?PCR-based approaches can be used to determine how much?DNA?is precipitated at a loc
Polyacrylamide－Gel－Electrophoresis－of－Oligonucleotides
1. Pour and polymerize a 20% polyacrylamide gel, no Urea.2. Remove clamps. Rinse with water. Remove comb. Rinse top of gel well.3. Insert comb teeth d
Blue－Native－Gel－Electrophoresis
Blue Native Gel ElectrophoresisStock solutions49.5%T, 3%C Acrylamide 24 g acrylamide, 0.75 g bisacrylamide / 50 ml H2O Store at RT3 x Gel buffer 150 m
Agarose－Gel－Electrophoresis－of－DNA
1) Dissolve 1 g of agarose in 100 ml of 1X TAE or TBE buffer (gives a 1% gel). See note for making LMP agarose gel.?2) Cast the gel with the comb in p
Alkaline－agarose－gel－electrophoresis
Alkaline agarose gel electrophoresis (Sambrook et al., 1989)Alkaline agarose gels can be used to determine the size and quality of first and second st
InGel－Digestion－of－Proteins－Separated－byPolyacrylamide－Gel－Electrophoresis
1. Excision of protein bands (spots) from polyacrylamide gelsRinse the gloves you use with water to avoid traces of dust in your sample.Rinse the gel
基于PCR技術的染色質沉淀分析1
INTRODUCTIONAfter chromatin immunoprecipitation (ChIP), different PCR-based approaches can be used to determine how much DNA is precipitated at a locu
Isolation－Of－PCR－Products
實驗概要Rapid and efficient purification of PCR products from salts, primers, dNTPs, and other non-nucleic acid reagents.實驗原理The ChargeSwitch? TechnologyT
SDS－Gel－Electrophoresis－of－Tubulin\MAPs
MaterialsStock Acrylamide: (30%T:0.8%C)30% by weight of acrylamide0.8% by weight of N,N'-bis-methylene acrylamideSeparation Gel (Final Concentrati
Denaturing－Gradient－Gel－Electrophoresis－(DGGE)
Purpose:Denaturing gradient gels are used to detect non-RFLP polymorphisms. The small (200-700 bp) genomic restriction fragments are run on a low to h
High－Resolution－Agarose－Gel－Electrophoresis
實驗概要Agarose gel ?electrophoresis remains the most widely used technique for separating ?nucleic acid fragments due to its ease of use, non-toxicity, a
Denaturing－Agarose－Gel－Electrophoresis－of－RNA
The overall quality of an RNA preparation may be assessed by electrophoresis on a denaturing agarose gel; this will also give some information about R
2D－Polyacrylamide－Gel－Electrophoresis
This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor
Native－gel－electrophoresis（非變性電泳）
Native gel electrophoresis?Under native PAGE conditions, polypeptides retain their higher-order structure and often retain enzymatic activity and inte
PEG－PRECIPITATION－OF－PCR－PRODUCTS
PEG PRECIPITATION OF PCR PRODUCTSThis protocol can be used instead of EXO/SAP for removing excess primers and nucleotides from PCR products before cyc
基因型分析
Randomly Amplified Polymorphic DNA (RAPD)Randomly Amplified Polymorphic DNA (RAPD)?by??(DNA KAFFE)RAPD analysis has been successfully used in mapping
DNA測序
DNA測序（主要內容如下）·?????????Sequencing Gel Preparation·?????????Preparation of Templates?·?????????DNA Sequencing by the Dideoxy Method·?????????DNA Sequen
PCR的下游應用
·?????????Agarose Gel Electrophoresis of PCR Products?(Robert H. Cruickshank)·?????????Agarose Gel Electrophoresis of PCR Products?(Immunology Resourc
DNA的凝膠電泳（gel－electrophoresis）
一、原理瓊脂糖或聚丙烯酰胺凝膠是分離和純化DNA片段的標準方法。聚丙烯酰胺凝膠電泳適用于分離小分子的核酸；瓊脂糖凝膠孔徑較大，被應用于大分子核酸的分離和純化。在一定濃度的瓊脂糖凝膠介質中，DNA分子的電泳遷移率與其分子量的常用對數成反比。當用低濃度的熒光嵌入染料溴化乙啶（EB）染色，在紫外光下至少可
QUALITATIVE－ANALYSIS－OF－DNA－FRAGMENTATION－BY－AGAROSE－GEL－ELECTROPHORESIS
1. IntroductionNuclear morphology changes characteristic of apoptosis appear within the cell together with a distinctive biochemical event: the endonu
TRFLP的優缺點
該技術在應用的過程中，肯定需要在實驗條件上不斷進行改進，而這種改進的好壞自然而然需要實驗結果的驗證。。V. Grüntzig在2002年做了該工作的一部分，結果認為，在限制性酶切時，很有必要去除其中影響DNA的酶切過程，并且實驗證明了具體的酶切時間。具有說服力的結果如下：??1，T-RFLP出數據的
TRFLP技術的優缺點
T-RFLP（末端限制性片段長度多態性）該技術在應用的過程中，肯定需要在實驗條件上不斷進行改進，而這種改進的好壞自然而然需要實驗結果的驗證。V. Grüntzig在2002年做了該工作的一部分，結果認為，在限制性酶切時，很有必要去除其中影響DNA的酶切過程，并且實驗證明了具體的酶切時間。具有說服力的