TUNELPROCEDUREINBOVINEEMBRYOS
Materials8% (w/v) paraformaldehyde stock solution: Dissolve 8 g of powdered paraformaldehyde in 100 ml water. Heat and stir (55-60 C – do not go higher). Add a few drops of 2 N sodium hydroxide until the solution clears. Make fresh each day. Alternatively, 8% paraformaldehyde can be purchased from Electron Microscopy Sciences as a custom formulation in 4 ml aliquots (cat. no. 157......閱讀全文
TUNEL－PROCEDURE－IN－BOVINE－EMBRYOS
Materials8% (w/v) paraformaldehyde stock solution:??Dissolve 8 g of powdered paraformaldehyde in 100 ml water. Heat and stir (55-60 C – do not go high
TUNEL－PROCEDURE－IN－BOVINE－EMBRYOS
Materials8% (w/v) paraformaldehyde stock solution:??Dissolve 8 g of powdered paraformaldehyde in 100 ml water. Heat and stir (55-60 C – do not go high
Tunel－Procedure－in－Bovine－Embryos－牛胚胎TUNEL檢測凋亡
Materials8% (w/v) paraformaldehyde stock solution:?Dissolve 8 g of powdered paraformaldehyde in 100 ml water. Heat and stir (55-60 C – do not go highe
發育生物學
In Vitro Production of Bovine Embryos?(P.J. Hansen Lab, Dept. of Animal Sciences, University of Florida)This protocol describe procedures for in vitro
Whole－mount－TUNEL－analysis－of－Xenopus－embryos
Fixation and pretreatmentDejelly albino embryos carefully in 2% Cystein (pH 7.8).Remove the vitellin membrane with two pairs of tweezers???????? (or c
Isolation－of－Microtubules－(Bovine－Brain)
LEVEL IIMaterialsFreshly removed bovine brain?2Wire sieve (tea strainer)Microtubule buffer (MT buffer)0.1 M MES (2-(N-Morphilino)ethanesulfonic acid)1
Fixation－of－Embryos
MEMFA Fix10xMEMFA Salts1 part 10x MEMFA salts1 M MOPS1 part 37% formaldehyde20mM EGTA8 parts water10mM MgSO410x salts can be autoclaved and stored. Tu
多功能胚胎激光破膜儀（二）
A new approach to cyropreservation of large equine embryos by vitrification after blastocoel micromanipulation.J. Scherzer, R. A. Fayrer-Hosken, L. Ra
In－Vivo－Luciferin－Imaging－Procedure
Mice are injected by an intraperitoneal route with a Luciferin solution (15 mg/mL or 30 mg/kg, in PBS, dose of 150 mg/kg) that is allowed to distribut
Gramstaining－Procedure
Gram-staining is a four part procedure which uses certain dyes to make a bacterial cell stand out against against its background. The specimen should
DNA－EXTRACTION－PROCEDURE－－GENERAL
Grow cells overnight in 500 ml broth medium.Pellet cells by centrifugation, and resuspend in 5 ml 50 mM Tris (pH 8.0), 50 mM EDTA.Freeze cell suspensi
Wholemount－staining－of－embryos
Fix embryos in formalin or MEMFA for one hour at room temperature with mixing. Rinse with TBS, replace with methanol, store at -20oC.Rehydrate by slow
Isolation－of－Zebrafish－embryos
Zebrafish will mate and deposit fertilized eggs on the bottom of the tank at 'dawn'. They can be accustomed to lay at any convenient time by k
Isolation－of－mouse－embryos
1. Sacrifice impregnated mouse.2. Dissect out the uterus of the mouse. Pulling up on the uterus with one set of forceps,use another to tear the mesome
Culture－of－BEND－Cells－(Bovine－Endometrial－Cells)
Culture of BEND Cells (Bovine Endometrial Cells)Charles E. Krininger, III and Peter J. Hansen?Dept. of Animal Sciences, University of FloridaThis prot
多功能胚胎激光破膜儀（一）
Laser-assisted derivation of human embryonic stem cell lines from IVF embryos after preimplantation genetic diagnosis.T. Turetsky, E. Aizenman, Y. G
細胞凋亡（TUNEL，TUNEL染色）檢測技術
細胞凋亡是指為維持內環境穩定，??生物體內細胞在特定的內源或外源信號誘導下，其死亡途徑被激活，并在有關基因的調控下發生的程序性死亡過程。細胞凋亡是程序性死亡過程的一種主要形式，它涉及染色質凝聚和外周化、細胞質減少、核片段化、細胞質致密化、與周圍細胞聯系中斷、內質網與細胞膜融合，最終細胞片段化形成許多
James－Hardwick－CNBr－Cleavage－Procedure
1. Immunoprecipitate the protein and run it on a preparative gel. CNBr cleavage must be done with protein?transferred?to a nitrocellulose filter. Neit
An－Integrative－Procedure－for－Apoptosis－Identification－and－Measurement
IntroductionApoptosis is a normal physiological phenomenon put forward by Kerr [1]. It plays an important role in embryonic development, maintenance o
TUNEL－方法
Terminal deoxynucleotidyl Transferase-mediated dUTP nick end labeling (TUNEL) is an?in situ?method for detecting the 3'-OH ends of DNA exposed dur
TUNEL－assay
PROTOCOL:?Deparaffinize and rehydrate slides:3 x 3′ Xylene3 x 2′ 100% ethanol1 x 2′ 95%, 80%, 70% ethanol (each)1 x 5′ 1x PBS?Microwave antigen retrie
TUNEL－labeling
In Situ Cell Death (Apoptosis) Detection by TUNEL labelingby Boehringer Mannheim (Catalog No. 1684809), modified by Josiah N. Wilcox andJosé C. Rodrig
Protocol－to－Count－Cell－Number－of－Preimplantation－Embryos
Protocol to Count Cell Number of?Preimplantation?Embryos?using Nuclear Staining with Hoechst 33342 or DAPI??Introduction?The following is a simple pro
In－vitroculture－of－early－chick－embryos
1. Use sterile technique. Prewarm Howard's Ringers in petri dish andagar/albumin culture dish to 37oC.2. Crack 2-day egg into large sterile petri
Sectioning－stained－embryos.
The protocols for plastic and wax sections as used by the Vize lab. These protocols work, but they have not been optimized. If anyone has better proto
Obtaining－Embryos:－hCG－Injection
Ovulation is induced in X. tropicalis by injection of human chorionic gonadotropin (hCG). X. tropicalis requires a much smaller dose than does X. laev
TUNEL分析實驗——組織切片的－TUNEL－染色
實驗材料組織試劑、試劑盒甲醛NaOHPBS二甲苯乙醇蛋白酶 KTris-HCl 溶液儀器、耗材Parafilm 膜實驗步驟1. 取下組織，立即用新制備的 4% 甲醛固定，室溫過夜。用多聚甲醛制備 4% 的甲醛溶液，在 100 ml 水中加 8 g 多聚甲醛，在通風櫥中加熱至 50~60℃，加幾滴 1
器官培養
In vitro organ cultures (Nagy Lab)kidneylungslimb??In Vitro Differentiation of ES Cells into: (Nagy Lab)Cardiac MuscleNeuronal LineagesCystic Embryoid
A－simple,－rapid－procedure－for－the－isolation－of－DNA－for－PCR
The polymerase chain reaction (PCR) is a method for amplifying specific segments of DNA defined by the small primers used to start the reaction. Using
A－simple,－rapid－procedure－for－the－isolation－of－DNA－for－PCR
N.M. DuTeau and J.F. Leslie - Department of Plant Pathology, Throckmorton Hall, Kansas State University, Manhattan, KS 66506-5502The polymerase chain
