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The mammalian calpain gene family currently contains 13 distinct large subunit products most of which complex with one of two smaller 30kDa subunits. ( An excellent introduction to the calpain family can be found on a web site created by Valery Thompson http://ag.arizona.edu/calpains/index.html ) One of the most carefully studied functions of the calpains is the regulation of integrin-mediated cell migration. Ca......閱讀全文
Flow cell assays are very useful for studying microtubule motility, microtubule dynamics, kinetochore-microtubule interactions and action of severing/
The hepatocyte growth factor receptor, also called c-Met, is activated by HGF and stimulates proliferation of hepatocytes and other cell types. Mutate
F. Video Microscopy 1. The behavior of migrating cells may be filmed with an inverted microscope fitted with a CCD camera. 2. Determine the
Rac-1 is a small G-protein in the Rho family that regulates cell motility in response to extracellular signals. Several changes in cytoskeletal struct
Flagella and motilitymonotrichous flagella - the bacterial cell has a single flagellaperitrichous flagella - the bacterial cell has several
Flagella and motilitymonotrichous flagella - the bacterial cell has a single flagellaperitrichous flagella - the bacterial cell has several
Interactions between cells responsible for cell to cell adhesion also can communicate signals into the cellular interior, often involving interactions
DAY 2: Cycling preparation of MT protein.Keep the brains in an evacuated plastic ziplock bag buried in ice from the time of slaughter during transport
Cyclin-dependent kinase inhibitor-2A (CDKN2A) goes by the colloquial designation p16, which is sometimes referred to as p16(INK4). The alpha transcrip
Dynamic Flow Assay in a Parallel Plate Flow ChamberJohn T. Patton~GlycoTech Corporation, Rockville, Maryland 20850Flow assays allow visualization of c
Dark Field ViewingPrincipleTo view a specimen in dark field, an opaque disc is placed underneath the condenser lens, so that only light that is scatte
羅納德·韋爾博士（Ronald D. Vale）是加州大學舊金山分校（UCSF）的細胞與分子藥理學教授。研究方向是驅動蛋白和動力蛋白分子馬達。他創造了一個科研界的世界記錄：一年內作為第一作者發 4 篇高質《Cell》論文，迄今無人打破。加上這 4 篇，他在研究生期間共發表 12 篇第一作
Migration of cells is involved in essential functions such as development, invasiveness of cancer cells, leukocyte movement toward chemotactic signals
The mammalian calpain gene family curently contains 13 distinct large subunit products most of which complex with one of two smaller 30kDa subunits. (
Mitochondrion-Selective Rhodamines and RosaminesRhodamine 123Rhodamine 123 (R302; FluoroPure Grade, R22420; Figure 12.22) is a cell-permeant, cationic
Fixation and Immunofluorescence of the Cytoskeleton (Mitchison Lab) Recycling Tubulin (Mitchison Lab) Labeling Tubulin
微電極陣列（Med-64）對野生型(WT)和W/W（v）型小鼠腸起搏器活動的研究Cajal間質細胞中的肌間神經叢區域（ICC-MyP）能形成一個網絡，并產生基底起搏電活動。這種形態特征使我們相信，這些細胞對胃腸道協調運動是非常重要的。我們希望找出一種新的方法來對ICC-MyP的電場活動和網絡功能作出
Integrins are cell surface receptors that interact with the extracellular matrix and mediate intracellular signals in response to the extracellular ma
Although many protocols for tubulin preparation are available, the procedure described below is the simplest and highest yielding preparation I have d
MSP, macrophage-stimulating protein, acts through the transmembrane receptor kinase RON (Stk in mice) to play a role in inflammation and the response
References1.Funatsu, T., Harada, Y., Tokunaga, M., Saito, K., and Yanagida, T. (1995) Imaging of single fluorescent molecules and individual ATP turno
人胚腎 293 (HEK293) 細胞在重組蛋白表達中是最常見的宿主細胞。 這類細胞能夠表達大量的膜蛋白，如 G 蛋白偶聯受體 (GPCR) ，是無法在最常見的生物制藥生產宿主，如：中國倉鼠卵巢 (CHO) 細胞中作表達。 HEK293 雖然是蛋白表達的極好宿主，然而 H
實驗概要Central neurons lose the ability for axonal regrowth during development and typically do not regenerate their axons following axotomy once the
AbstractTerahertz (THz) metasurfaces have been explored recently due to their properties such as low material loss and ease of fabrication
ContentsEmbryonic Stem Cell MarkersHematopoietic Stem Cell MarkersMesenchymal/Stromal Stem Cell MarkersNeural Stem Cell MarkersReferencesWhile stem ce
實驗概要Directed differentiation of specific lineages has been a focal point in the field of human embryonic stem cell (hESC) research. Cell r
Mammalian RNA InterferenceThomas TuschlLaboratory for RNA Molecular BiologyThe Rockefeller University, New York Excerpted from RNAi: A Guide
The onion root tip and the whitefish blastula remain as the standard introduction to the study of mitosis. The onion has easily observable chromosomes
實驗概要The LIVE/DEAD? Fixable Dead Cell Stain Kits use a novel method to evaluate the viability of mammalian cells by flow cytometry. These a
實驗概要A novel impulsive cell pressurization experiment has been developed using a Kolsky bar device to investigate blast-induced traumatic b