UseoftheB.D.FACSorCaliburFlowCytometers
Reservations:Schedule time on a cytometer (FACS or Calibur) by filling in the calendar at the flow lab. Don't grossly overbook since we have to pay for both reserved time and the time actually used (which ever is greater).Prepare Cells:Cells should be stained ahead of time and suspended as single cells in PBS to a density of about 20,000 to 100,000 cells/mL in flow tubes (see separate staining protocols). Pass ce......閱讀全文
Use of the B.D. FACS or Calibur Flow Cytometers
Reservations:Schedule time on a cytometer (FACS or Calibur) by filling in the calendar at the flow lab. Don't grossly overbook since we have to pa
Use of the B.D. FACS or Calibur Flow Cytometers-2
Gating:Gating will allow you to view cells of interest by any combination of criteria that you choose. Gating does not change the intensity value assi
Detection Of Cell Viability And/Or Apoptosis By Flow Cytometry (FACS)
Viable?cells are cells that when allowed to continue beyond the timepoint of examination will stay alive. Besides live and healthy cells, cells in ear
流式細胞術
一.?流式細胞術概述流式細胞術(Flow?Cytometry,?FCM)是七十年代發展起來的高科學技術,它集計算機技術、激光技術、流體力學、細胞化學、細胞免疫學于一體,?同時具有分析和分選細胞功能。它不僅可測量細胞大小、內部顆粒的性狀,還可檢測細胞表面和細胞漿抗原、細胞內DNA、RNA含量等,可對
流式細胞儀主要構造和工作原理（一）
一.?流式細胞術概述 流式細胞術(Flow?Cytometry,?FCM)是七十年代發展起來的高科學技術,它集計算機技術、激光技術、流體力學、細胞化學、細胞免疫學于一體,?同時具有分析和分選細胞功能。它不僅可測量細胞大小、內部顆粒的性狀,還可檢測細胞表面和細胞漿抗原、細胞內DNA、RNA含量等,可
流式細胞儀主要構造和工作原理
一. 流式細胞術概述? 流式細胞術（Flow Cytometry, FCM）是七十年代發展起來的高科學技術,它集計算機技術、激光技術、流體力學、細胞化學、細胞免疫學于一體, 同時具有分析和分選細胞功能。它不僅可測量細胞大小、內部顆粒的性狀,還可檢測細胞表面和細胞漿抗原、細胞內DNA、RNA含量等,
流式細胞儀主要構造和工作原理
一.?流式細胞術概述???? 流式細胞術（Flow?Cytometry,?FCM）是七十年代發展起來的高科學技術,它集計算機技術、激光技術、流體力學、細胞化學、細胞免疫學于一體,?同時具有分析和分選細胞功能。它不僅可測量細胞大小、內部顆粒的性狀,還可檢測細胞表面和細胞漿抗原、細胞內DNA、RNA含
Flow Cytometry Analysis
PurposeFlow cytometry employs instrumentation that scans single cells flowing past excitation sources in a liquid medium. The technology can provide r
Use of a Hemacytometer
A hemacytometer (also spelled hemocytometer) is an etched glass chamber with raised sides that will hold a quartz coverslip exactly 0.1 mm above the c
Use of Micropipets
A. Gilson-style Pipetmen Micropipets (and similar models)We primarily use the Gilson micropipets in the core course labs. We have four sizes identifie