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PurposeFlow cytometry employs instrumentation that scans single cells flowing past excitation sources in a liquid medium. The technology can provide rapid, quantitative, multiparameter analyses on single living (or dead) cells based on the measurement of visible and fluorescent light emission. Flow cytometry is a widely used method for characterizing and separating individual cells. This basic protocol focuses on: me......閱讀全文
最方便的實驗干貨查詢工具微信掃碼進入「丁香實驗」小程序編輯： 嗚咽分享到： Flow Cytometry Analysis (Springer Lab, Harvard University)Flo
Flow Cytometry Analysis (Springer Lab, Harvard University) Flow cytometry employs instrumentation that scans single cells flowing past excit
外周血是臨床檢驗中的重要標本。FCM分析外周白細胞的主要目的是了解各種白細胞的數目與分群情況。這些數字的變化與臨床的某些疾病有一定的關系。近年來，由于多種識別白細胞膜表面抗原的單克隆抗體的發現，以及對這些單克隆抗體的直接或間接熒光標記物的出現，使得利用FCM的熒光組織化學分析獲得被測細胞的多指標的更
實驗概要The LIVE/DEAD? Fixable Dead Cell Stain Kits use a novel method to evaluate the viability of mammalian cells by flow cytometry. These a
B.3. COMMENTARY B.3.1 Background information The critical steps in the methodology are cell fixation, permeabilization and the concentration
MaterialsCytokine-specific Primary Antibodiesunlabeled or biotinylated antigen-affinity purified polyclonal antibodies (R&D Systems ''AF&#
Flow Cytometric AnalysisPrior to flow cytometric analysis, the vital dye Hoechst 33342 (Sigma-Aldrich, St. Louis, MO, USA) was added to cell suspensio
Simultaneous analysis of DNA content and surface immunophenotype using gentle ethanol fixation techniques. William Telford. Louis E. King a
TroubleshootingCritical Steps(1) Don’t trypsinize cells for too long when collecting them.(2) Rotation speed should be no more than 1500 rpm during ce
Flow cytometry analysis of the quantitative expression of 3 key pluripotency-associated transcription factors (Nanog, OCT3/4 and SOX2) complemen
DNA and RNA Staining6. Stain cells with 7-AAD: i. Resuspend the cells from Step 5 in 0.5 mL of NASS containing 10 μg/mL of 7-AAD. Incub
實驗概要The blue-fluorescent DAPI nucleic acid stain preferentially stains dsDNA; it appears to associate with AT clusters in the minor groove
MATERIALS:1. 1 X PBS (PBSAz, 1 X PBS, e.g., Irvine Scientific, CA, containing 2% newborn calf serum and 0.1% sodium azide)2. 7-Amino-actinomycin D (7-
實驗概要Live cell studies of cellular DNA content and cell cycle distribution are useful to detect variations of growth patterns due to a vari
Mario Roederer博士的團隊通過研究單細胞來更好地描述在HIV治療過程中免疫系統的特征。該團隊使用非人類靈長目模式動物，用單細胞基因表達分析提高T細胞的鑒定。他們使用了先進的技術，包括18色 FACS系統和Fluidigm BioMark? 系統進行單細胞基因表達分析。基本研究內容包括：
Protocol 3: Fetal Liver-Derived HSC Differentiation on OP9-DL1 CellsDay 0: Initiation of Fetal Liver Co-culture52. Isolate liver tissue from eight to
Moreover, when the method was applied to the analysis of the cellular composition of immature testes, the results were also in agreement with previous
實驗概要Live cell studies of cellular DNA content and cell cycle distribution are useful to detect variations of growth patterns due to a vari
THE CELL 1. Morphological aspects of apoptosis Walter Malorni, Stefano Fais & Carla Fiorentini 2. Cell cycle Miriam
實驗概要The blue-fluorescent DAPI nucleic acid stain preferentially stains dsDNA; it appears to associate with AT clusters in the minor groove
Hematopoietic Stem Cell Targeting with Surface-Engineered Lentiviral VectorsEls Verhoeyen and Fran?ois-Lo?c CossetAdapted from Gene Tra
Freezing ESCs 25. Passage the ESCs as described in Step 22. Resuspend the cells in 3 mL of freezing medium for OP9 cells. 26. Aliquot&n
PROPIDIUM IODIDE: The most commonly used dye for DNA content/cell cycle analysis is PROPIDIUM IODIDE (PI). It can be used to stain whole cells or isol
實驗概要The CellTrace? CFSE Cell Proliferation Kit provides a versatile and well-retained cell-tracing reagent in a convenient and easy-to-use
1、eBioscience公司提供了哪些抗體？ A：物種——人、小鼠、大鼠、非人靈長類、犬類等。檢測指標——包括細胞表面標記（CD分子，膜骨架，趨化因子受體）和胞內指標（細胞因子，核內轉錄因子）。抗體標記——生物素標記抗體，親和純化抗體以及直標熒光素抗體。eB抗體的直標熒光素有（括號內是
實驗概要A modification of the basic immunofluorescence staining and flow cytometric analysis protocol can be used for the simultaneous analysi
Table 2: Human Cytokines: Intracellular Staining Quick GuideHuman Cytokines: Intracellular Staining Quick GuideHuman CytokineCell SourceActivationIncu
Labeling Microtubules (Molecular Dynamics Inc. )Microtubules are involved in many aspects of cell motion including propulsion, mitosis
Figure 5: hMSC proliferation rate during long-term expansion in di?erent animal-component-free culture systemshMSC expansion on the FN1 motifs surface
實驗概要Researchers today are trying to maximize the information that they get out of flow cytometry experiments by looking at more parameters