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OverviewIn order to study spleen cells (e.g. lymphocytes, granulocytes, other immune cells), it helps to make single-cell suspensions so that the cells can be manipulated ex vivo easily. This protocol suggests ways in which you can do this without a lot of equipment or expensive supplies. This protocol can also be used to make cell suspensions from other lymphoid organs, such as the thymus or lymph nodes (see Current......閱讀全文
Intestinal intraepithelial lymphocytes Intestinal intraepithelial lymphocytes (IELs) are mostly T cells, which are continuously
ContentsEmbryonic Stem Cell MarkersHematopoietic Stem Cell MarkersMesenchymal/Stromal Stem Cell MarkersNeural Stem Cell MarkersReferencesWhile stem ce
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In VitroRoxanne Holmes and Juan Carlos Zú?iga-Pfl
DNA抽提（主要內容如下）· Working with DNA· DNA Extraction from Bacteria and Other Organisms· DNA Extraction f
DNA PreparationGene TransferEmbryo TransferTransgenic IdentificatioinOthersTransgenic Outline (University of Michigan Transgenic Animal Model Cor
Media and Solution required for ES Cell Culture (Bowtell Lab) Routine Culturing of ES Cells (Bowtell Lab) Routin
MaterialsTrypsin (Gibco 25200-023)3T3 Medium: 500 mL DME (Invitrogen) + 50 mL FBS (Hyclone) + 5 mL 100x Pen/Strep2x Freezing Medium: 3T3 Medium
4 L型細菌的耐藥性與醫院感染 細菌變成L型后與其原菌株不同，故臨床治療用藥時，不僅要考慮細菌的藥敏，也要考慮L型的藥敏。一般認為細菌變為L型后已經失去了細胞壁，而β-內酰胺抗生素(青霉素類抗生素)的作用主要是抑制肽聚糖的合成，所以對細菌L型不起作用，而對作用于核酸與蛋白質合成的藥物如紅霉素、氯霉
As a primary component of circulatory system, serum is the major reservoir of thousands of proteins secreted or “leaked” from a broad spectrum of
cDNA LibrariesIsolation of corresponding genetic informationInstead of synthesizing a desired gene, can we used the amino acid information to directly
Gene Targeting Outline (University of Michigan Transgenic Animal Model Core)This is a brief outline of the steps necessary to produce mice with a
RNA提取（主要內容如下）Tips for Handing RNA Total RNA IsolationmRNA IsolationrRNA IsolationOthersQ & A posted in the Method Forum Basic Proc
實驗概要DNAzol? Reagent (Genomic DNA Isolation Reagent) is a complete and ready-to-use reagent for the isolation of genomic DNA from solid and
B. Midiprep double-stranded DNA isolationA midi-prep double-stranded DNA isolation has been developed to generate a sufficient amount of template DNA
DNA電泳（主要內容如下） Preparation of Agarose Gel and Electrophoresis Extraction of DNA From Agarose Gel Extraction of DNA fro
DNA Extraction· DNA Extraction from Bacteria (Julie B. Wolf,UMBC)Phenol/chloroform method·&n
實驗概要Human embryonic stem cells can be differentiated into neural-, mesenchymal and hematopoetic stem cells. This product is intended for t
DNA Isolation by a Cleared Lysate Method Followed by Double Acetate Precipitation Version 3b - updated September 26, 1999The Most Recent Roe Lab Imple
實驗概要Plant DNAzol? is an extra-strength-DNAzol? reagent (patent pending) specifically formulated for the isolation of genomic DNA from plants. The Plan
This procedure describes a convenient method for the isolation of crude nuclear pellets from N. crassa. The method, an adaptation of the one deve
Adrenal chromaffin granule (chromaffin vesicle) preparationIntroduction. This prep is adapted from the classic paper of Smith and Winkler (Smith
Western 雜交（主要內容如下）Preparing of Protein LysatesWestern BlottingFar Western BlottingSemi Dry BlottingStripping MembranesTrouble Shooting and Others
實驗概要 This protocol utilizes the powerful guanidine isothiocyanate–phenol:chloroform extraction method which allows the
蛋白質提取和純化（主要內容如下）Protein Extraction Protein PurificationProtein PrecipitationColumn PreparatioinQ & A Posted in the Method ForumProtein Extrac
Protocols for LCM preparation and analysis I. Preparation, LCM and RNA/DNA extraction of Frozen Tissue SectionsA. EmbeddingB. CuttingC.
Megabase DNA IsolationMegabase-size DNA isolation from plantsTo construct large insert DNA libraries in BAC and YAC vectors, methods must be developed
實驗概要The Dynabeads? and buffers provided in this kit will enable you to a) covalently immobilize antibodies of your choice onto the surface
Methods Materials. A uniform mixture of six standard glycoproteins was used as the starting material, including IgG (56834, catalog numeber),
實驗概要Native chromatin immunoprecipitation to query specific chromatin states of individual genes. 主要試劑10 x TBS 0.1 M Tris-HCl (pH 7.5) 1.5 M NaCl