LiveimagingwithDrosophilatissueculturecells1
IntroductionLive imaging provides an important complementation to the "snapshot" view obtained in fixed tissue by immunofluorescence. It allows following dynamic cellular processes as they unfold "in vivo", and often reveals a degree of complexity impossible to study in still images. Thanks to the increasing interest in this technique, significant technical improvements have been made in the recen......閱讀全文
Live imaging with Drosophila tissue culture cells-1
Introduction Live imaging provides an important complementation to the "snapshot" view obtained in fixed tissue by immunofluorescence. It allows fol
Live imaging with Drosophila tissue culture cells-2
Materials & Reagents Drosophila?Schneider S2 cells Schneiders Medium (GIBCO/Invitrogen), 10% fetal calf serum, Antibiotics (Sigma A5955)
Immunofluorescence Microscopy of tissue culture cells
Immunofluorescence Microscopy of tissue culture cells These methods are written for direct staining of filamentous actin with bodipy FL-phallicidin
Tissue Culture of PtK1 cells
Please note: lately (2005 - present), we normally culture PtK1 cells in F-12 media, but they also grow in other types of media as described below.
Isolating Xenograft Tumor Cells for Tissue Culture or Transplantation
Isolation of T4 cells from xenograft tumors ? Put mouse down Wash skin by dipping mice into ETOH Remove tumor and put into 5ml PBS containi
Live Cell Imaging of Yeast
Live Cell Imaging of Yeast Daniel R. Rines, Dominik Thomann, Jonas F. Dorn, Paul Goodwin and Peter K. Sorger INTRODUCTION The development of clonin
Tissue Culture Media
We use two different kinds of media. Most cells are grown in DMEM. A few lymphoid cell lines are grown in RPMI. Cells grown in DMEM must be grown in
TISSUE CULTURE ON COVERSLIPS
I.?Purpose:A. Skin tissue may be used for chromosome analysis in special cases when the results from peripheral blood are inconclusive, e.g. suspected
Immunofluorescent Staining of Drosophila Larval Brain Tissue
INTRODUCTION The?Drosophila?larval brain is a well-established model for?investigating the role of stem cells in development. Neuroblasts?(neural st
Immunofluorescent Staining of Drosophila Larval Brain Tissue
實驗概要The Drosophila larval brain is a well-established model for investigating the role of stem cells in development. Neuroblasts (neural stem cells)