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IntroductionLambda phage is a commonly used vector for transgenes. Its very high rate of infectivity makes it ideal for creating large numbers of clones. Large numbers of clones are often needed for construction of gene libraries that represent large populations of molecules. These libraries are also easy to sort through, as lambda phage clones are particularly easy to array spatially at high density. This is called ......閱讀全文
Outline:Bacteriophage lambda is a linear double stranded DNA, approximately 50 kB in size. The two sticky ends help the phage to recircularize after e
Easy Way to Clone The protocol is oriented towards a C. albicans genomic library I made in Lambda Zap II on 7/97.The overall sequence of events i
PREPARE SOLUTIONS1. 10mM MgSO4, 0.2% Maltose LB (100 mL):Mix 1.0 g of Bacto-Tryptone, 1.0 g of NaCl, 0.5 g of Yeast Extr
CDNA文庫（主要內容如下）· Construction of cDNA Library· &nbs
Preparing Lambda DNA1- Coliphage lambda DNA is a widely used vector for recombinant DNA. The middle third of its 48,000 bp contains no genes required
Screening a cDNA Libraryfor use with HybriZAP zebrafish cDNA librariesObjectivecDNA library screening allows detection of expressed genes for subseque
David HarryInstitute of Forest GeneticsUSDA Forest ServicePacific Southwest Research StationAugust 26, 1993Background :There are many published method
Preparation of Phage LysatesInoculate 5 ml of lambda-broth in a glass culture tube with a single colony of an appropriate host strain of E. coli.
HELPER PHAGE PREPARATION1. Grow an overnight of NM522 in NZCYM medium.2. Dilute overnight 1:100 and grow to an A600 = 0.3 (@2.5 x 108 cells/ml).3. Inf
IntroductionThe phage lysate from the plate contains bacterial DNA and RNA, as well as phage DNA encased in the phage coat. The following procedure, d
Cosmid Cloning: Cell preparation, DNA packaging, and Cell TransfectionProtocol taken from Stratagene's Gigapack packaging extracts instruction man
Preparing Lawn Cells for M13 Cloning (Life Technologies)Lawn cells require the F' episome for M13 infection and may be prepared St
熒光偏振法快速、準確、高通量檢測IgG和含Fc段衍生物Dr. Carolanne DohertyValitacell, NIBRT, Fosters Avenue, Blackrock, Dublin, Ireland BMG多功能酶標儀可應用于定量IgG的新技術Valit
I. Introduction:This assay uses a lawn of phage-susceptible E. coli (DH10B) embedded in a layer of agarose. This top agarose lays on a bed o
Genomic DNA libraries Size of some genomes and chromosomes:Comparative Sequence Sizes(Bases)(yeast chromosome 3)350 ThousandEscherichia coli (bac
Pick up one phage vectors-containing colony with a sterile loop and put into 10 ml 2xTY + 10 μg/l tetracycline.Shake at 200 rpm and 37 °C untill
· M13 Phage (Michael Blaber)Very useful background information about M13: its infection, rep
Purpose:Mini-prep method for lambda phage DNA purification from lysates.Time required:4 hours once the lysate is in handSpecial supplies required:BioR
The restriction/modification system in bacteria is a small-scale immune systemfor protection from infection by foreign DNA. W. Arber and S.
· Lambda DNA Preparation (Stanford DNA Sequence & Technology Center)Detailed protocol fo
Table 2: Binding sites and identity of ZFPs used in VEGF activationWe then generated artificial transcription factors by fusing the three-fi
SummaryCleaner stocks of T7 that concentrates and purifies T7 bacteriophage.ProtocolGrow 100mL of permissive cells to a density of 108 to 109&nbs
Human zinc fingers as building blocks in the construction of artificial transcription factorsKwang-Hee Bae1, 4, Young Do Kwon1, 2, 4, Hyun-Chul Shin1,
Part A. Isolation of Nucleic AcidsNOTE: CAUTION! STEPS 1-10 SHOULD BE PERFORMED USING APPROPRIATE PROCEDURES FOR HANDLING MATERIAL POTENTIALLY CONTAMI
基因克隆的常用方法 基因(gene)是遺傳物質的最基本單位,也是所有生命活動的基礎。不論要揭示某個基因的功能,還是要改變某個基因的功能,都必須首先將所要研究的基因克隆出來。特定基因的克隆是整個基因工程或分子生物學的起點。本文就基因克隆的幾種常用方法介紹如下。
超級細菌 這種細菌會充滿像Mallory這樣的囊性纖維化病人的肺部，在粘液中生存，但并不妨礙她的生活。于是13年期間，細菌伴隨著她讀完高中，伴隨她參加學校的水球、排球和游泳隊；又隨她進入斯坦福大學（Stanford University），在她學習生物學或是打排球時和平共處。她甚至還寫完了一本
SummaryHow to make a lysate from a plaque preparation. We also use this protocol for preparation of a quick stock from previously made lysate prep.Pro
Day 0Make sure you have the necessary solutions (instructions for how to make them can be found here):Single-stranded carrier DNAPEG 3350 50% w/vol1.0
實驗概要The E.Z.N.A.? family of products is an innovative system that radically simplifies the extraction and purification of nucleic acids fr
通過以血清的各種反應為中心的機體免疫反應和變態反應的研究體系來篩選新抗原和新基因。實驗材料大腸桿菌試劑、試劑盒TBST 裂解液 血清 NZY top IPTG 一抗 二抗 SM 封閉液 MgSO4 LB AP BCIP-NBT 顯色液儀器、耗材硝酸纖維素膜 濾紙 冰箱 培養箱 水浴鍋 平板 脫色搖床