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Outline:Bacteriophage lambda is a linear double stranded DNA, approximately 50 kB in size. The two sticky ends help the phage to recircularize after entering the bacterial host cell. A cDNA library is a representation of mRNA's that are expressed in a certain tissue at a certain time (e.g. rat d9 pseudopregnant decidua). Each phage contains one cDNA that was 'grabbed' by the poly-A tail and cloned into a ......閱讀全文
2.2 Interaction mating - large scaleWith a few modifications, the procedure described above can be used to test for interactions between a single prey
Figure 5: Regulation of CDH5 by TFZFs in several human cancer cell lines.Blue, cells infected with a pMX construct containing the DNA bindin
Scanning the human genome with combinatorial transcription factor librariesPublished online: 18 February 2003, doi:10.1038/nbt794March 2003 Volume 21
Human zinc fingers as building blocks in the construction of artificial transcription factorsKwang-Hee Bae1, 4, Young Do Kwon1, 2, 4, Hyun-Chul Shin1,
Table 2: Binding sites and identity of ZFPs used in VEGF activationWe then generated artificial transcription factors by fusing the three-fi
AbstractWhen more than one bait will be used to screen a single library, significant time and resources can be saved by performing the interactor hunt
INTRODUCTIONThis protocol describes a method for removing antibodies that react with bacterially encoded proteins by passing a crude preparation of im
1. Introduction and BackgroundThere is a great need for general methods to characterize the proteins that contemporary biology makes available. The li
生物分子發表的代表性文獻 QTRAP：同時具有三重四極桿和線性離子阱性能的獨一無二的LC/MS/MS系統 QTRAP系統最早在ASMS 2002上，作為第一臺商用的線性離子阱發布，是世界上唯一的線性離子阱和三重四極桿的復合
Using siRNA for gene silencing is a rapidly evolving tool in molecular biology. There are several methods for preparing siRNA, such as chemical synthe
Screening Kinase Phosphorylation Motifs Using Peptide LibrariesIsaac A. Manke and Michael B. YaffeCenter for Cancer Research, Massachusetts Institute
CDNA文庫（主要內容如下）· Construction of cDNA Library· &nbs
Interaction Trap/Trap Two-Hybrid System· Yeast Two-Hybrid System (Finley Lab)This is one of
Target analysis by Parallel reaction monitoring (PRM)The targeted quantification and verification were carried out in 94 serum samples with 5~
References1. Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J, Jemal A. Global cancer statistics, 2012. CA: a cancer journal for clinician
諾華（Novartis）制藥 Wang, Karen（王瑛琪）教授 來自諾華（Novartis）制藥的Wang, Karen（王瑛琪）教授，做了題為“Application of Mass Spectrometry in Pharmaceutical Drug Discovery Research
Purpose and BackgroundsCHO lec 3.2.8.1 cellsCHO Lec 3.2.8.1 cells have four independent mutations in the N- and O- glycosylation pathways (Stanley, 19
生物標志物之于腫瘤轉化醫學的意義：從篩選、確證走向臨床應用醫學是否能夠有效的治療及治愈癌癥直接取決于是否能夠在癌癥早期階段對其進行及時的檢測。生物標志物作為最直接快速有效的診斷手段，其篩選與獲得可在腫瘤診斷、發展、治療、以及療效監測等多個方面發揮重要的作用。因此，自該概念的提出，就受到了極大的關注，
References1. Fujita T, Kozuka-Hata H, Ao-Kondo H, Kunieda T, Oyama M, Kubo T: Proteomic analysis of the royal jelly and characterization of the fu
瑞士蘇黎世聯邦理工學院的研究人員領導的一個研究小組，已制定了一個質譜工作流，用于對整個蛋白質組進行高通量的絕對定量。 據ETH的研究者、該研究的領頭人Ruedi Aebersold說，對于多種形態，該技術實現了目標蛋白質組的重現性定量，提供用于
應用WGS正在成為NGS中最廣泛的應用。通過該技術并且結合生物學應用，研究人員可以獲得基因組信息中最值得注意的信息73。舉例來說，2012年，Ellis等報道了基因與乳腺癌患者芳香酶抑制劑（aromatase inhibitor）治療法之間的關聯。他們指出突變，后果與診斷之間的關聯，同樣還有
In Vivo Imaging of Far-red Fluorescent Proteins after DNA Electrotransfer to Muscle TissueDNA electrotransfer to muscle tissue yields long-term, high
Preparation of Glutathione-S-Transferase (GST) Fusion ProteinsMargret B. Einarson and Elena N. Pugacheva Foxx Chase Cancer Center, Philadelphia,
Metabolic biotinylation of mammalian cell receptors for imagingBakhos A. Tannous , btannous@hms.harvard.edu, Massachusetts General Hospital and Harvar
Cryo-thermal therapy induced an “acute” phase response First, we performed PRM analysis upon significant proteins involved in acute phase respo
一個經典的蛋白芯片操作流程：Experimental Procedures for Protein Microarrays--------------------------------------------------------------------------------Chemicall
人胚腎 293 (HEK293) 細胞在重組蛋白表達中是最常見的宿主細胞。 這類細胞能夠表達大量的膜蛋白，如 G 蛋白偶聯受體 (GPCR) ，是無法在最常見的生物制藥生產宿主，如：中國倉鼠卵巢 (CHO) 細胞中作表達。 HEK293 雖然是蛋白表達的極好宿主，然而 H
Flow cytometrySpleens were extracted after therapy and mechanically homogenized with gentle MACS Dissociator to obtain a single-cell splenocyte su
In summary, we suggested that “acute” response could elicit innate and Th1 adaptive immunity, reversing the tumor-mediated immunosuppressive envir
Figure 1: epMotion 5073l worktable for qPCR setup Results and DiscussionThe automation of the qPCR setup using the 10 μL dispensing tool a