WIKI資訊
Grow cellsHarvestSpin downWash with PBSSpin down enough cells to collect a 50-100 μL cell pellet in a FastPrep tubePrepare lysis buffer (beforehand) and chill on icePMSF: 1 mL per 100 mL bufferPLAAC: 100 μL per 100 mL buffer0.5 M Benzamidine: 260 μL per 100 mL bufferUse fresh PMSF: dissolve 0.035g per 1mL 100%EtOH2.5 mM MgCl23 mM KCl0.5% Triton X-100in PBSPBSMT:Add protease inhibitorsAdd 50-100 μL of cold lysis buffe......閱讀全文
WESTERN BLOT PROTOCOLIn a Western blot, proteins that are separated on polyacrylamide gels on the basis of size are transferred to a membrane for dete
This page should point you to the many different general and lab-specific protocols describing tissue and cell lysis and serve as forum for comparison
實驗概要The Dynabeads? and buffers provided in this kit will enable you to a) covalently immobilize antibodies of your choice onto the surface
實驗概要The Dynabeads? and buffers provided in this kit will enable you to a) covalently immobilize antibodies of your choice onto the surface