WIKI資訊
Quantitation of DNADetection of Nucleic Acids Using Absorption Spectroscopy The absorption of the sample can be measured at several different wavelengths to assess the purity and concentration of nucleic acids, as follows: A260---Quantitative for pure nucleic acids in microgram quantitiesA260/A280---Indicator of nucleic acid purityA325---Indicates particulates in solution or dirty cuvettes1) Turn on the UV ......閱讀全文
Western 雜交（主要內容如下）Preparing of Protein LysatesWestern BlottingFar Western BlottingSemi Dry BlottingStripping MembranesTrouble Shooting and Others
DNA抽提（主要內容如下）· Working with DNA· DNA Extraction from Bacteria and Other Organisms· DNA Extraction f
RNA提取（主要內容如下）Tips for Handing RNA Total RNA IsolationmRNA IsolationrRNA IsolationOthersQ & A posted in the Method Forum Basic Proc
CDNA文庫（主要內容如下）· Construction of cDNA Library· &nbs
In Situ Hybridization· In Situ Hybridization (jsmith1@po-box.mcgill.ca)In situ hybridization
蛋白質提取和純化（主要內容如下）Protein Extraction Protein PurificationProtein PrecipitationColumn PreparatioinQ & A Posted in the Method ForumProtein Extrac
DNA轉化Chemical Transformation· Transformation of Competent Cells (RbCl2 Method) (Goldberg Lab
· Standard PCR Protocol (Molecular Biology Techniques Manual)The followings are described in
· Standard (alkaline lysis) Mini-Prep (Goldberg Lab)Standard protocol for mini-prep and reci
· Standard (alkaline lysis) Mini-Prep (Goldberg Lab)Standard protocol for mini-prep and reci
Media and Solution required for ES Cell Culture (Bowtell Lab) Routine Culturing of ES Cells (Bowtell Lab) Routin
DNA測序（主要內容如下）· Sequencing Gel Preparation· P
DNA標記（主要內容如下） DNA Labeling by Nick Translation Random Primed Labeling End-Labeling Purification of Labeled
· What's PCR? (Michael Blaber's Lab)Illustrated introduction to usr/localious aspect
What's PCR? (Michael Blaber's Lab)Illustrated introduction to usr/localious aspects of PCR technique. It's very valuable not onl
Interaction Trap/Trap Two-Hybrid System· Yeast Two-Hybrid System (Finley Lab)This is one of
Fine Mapping of Genomic Targets of Nuclear Proteins in Cultured CellsAchim Breiling and Valerio OrlandoDulbecco Telethon Institute, Institute of Genet
蛋白質電泳（主要內容如下）One-Dimensional SDS-PAGETwo-Demensional SDS-PAGEProtein Electrophoresis in Agarose Gel Gel StainingRecipesOne-Dimensional SDS-PAGE·&
· Yeast Transformation (Gietz Lab)LiAc/SS-DNA/PEG Transformation· &nb
DNA PreparationGene TransferEmbryo TransferTransgenic IdentificatioinOthersTransgenic Outline (University of Michigan Transgenic Animal Model Cor
· Yeast Two-Hybrid System (Finley Lab)This is one of the most comprehensive and detailed gui
DNA轉染· Transfection of Mammalian Cells Using Lipofectamine (LTI)· &n
實驗概要The iPrep? GeneCatcher? gDNA Blood Kit allows rapid and automated extraction of genomic DNA (gDNA) from human blood including archived
· Tips and hints for the storage of antibodies (Synaptic Systems)· &n
Southern雜交One important thing for transfer:the weight of the object resting on top of the blotting apparatus should not exceed the weight equal to a 5
In this section, you will find techniques related to oligonucleotides, such as oligo purification by acrylamide gel, annealing two oligos to make doub
Take the appropriate set of Fmoc-amino acid stock aliquots for cycle 1 from the freezer, bring to room temperature, and activate by adding DIC (4 μl p
Antibody PurificatioinPurification of IgG Using Protein A- or Protein G-Agarose (KPL) Purifying Antibodies (Perkin-Elmer)Precipitation
OverviewThis is a method to assemble two BioBricks using the Clontech In-Fusion PCR Cloning Kit and maintains BioBrick standard formats. There are cur
From the various microsurgical procedures of Spemann and others, several developmental principles have emerged about amphibian embryos. One of the mos