HighThroughputIsolationOfPCRProductsUsingChargeSwitch?PCRCleanUp
實驗概要The ChargeSwitch? PCR Clean-Up Kit allows rapid and efficient purification of PCR products from salts, primers, dNTPs, and other non-nucleic acid reagents. The kit is designed for automated processing of large numbers of samples in 96-well plates using a liquid handling robot. The kit is designed for the purification of DNA fragments ranging from 90 bp-40 kb and the purified PC......閱讀全文
Isolation of papillary cells
Isolation of renal papillary cells1.?For isolation of papillary cells, kidneys were harvested and kept in HBSS containing 15 mM HEPES, penicillin/
DNA isolation extraction
CTAB TECHNIQUE / Method / Schedule / Protocol FOR DNA ISOLATION / DNA EXTRACTION FROM PLANT LEAF / LEAVES SAMPLES (see also DNA RNA double isolati
Isolation of murine splenocytes
Overview In order to study spleen cells (e.g. lymphocytes, granulocytes, other immune cells), it helps to make single-cell suspensions so that the c
Isolation of Zebrafish embryos
Zebrafish will mate and deposit fertilized eggs on the bottom of the tank at 'dawn'. They can be accustomed to lay at any convenient time by keeping
Isolation of mouse embryos
1. Sacrifice impregnated mouse. 2. Dissect out the uterus of the mouse. Pulling up on the uterus with one set of forceps, use another to tear the mes
Isolation of colonic epithelium
實驗概要 The ?method we use is based on work of Dr. Hazel Cheng, at the University of ?Toronto and works for both colon and the small intestine. First
RNA Isolation Protocol
Stabilize RNA Start with 15 ml E. coli Culture containing 7.5* 109 cells (OD600= 0.2 Dilute cells or scale up) Pipet 30 ml of RNAProtect Bacteria
Chromosomal DNA Isolation
Chromosomal DNA Isolation METHOD: Grow cells in 2-5 ml broth to late log phase. Pellet 1-2 ml cells in microfuge. Resuspend cells in 400
Cosmid DNA Isolation
實驗概要 Isolation of high yields of highly pure cosmid DNA using PureLink? HiPure Plasmid Purification Kits. 實驗原理 The ?PureLink? HiPure Plasmid Purif
Isolation of papillary cells
實驗概要 This protocols provides a general protocol for isolation of papillary cells. 實驗步驟 Isolation of renal papillary cells 1.?For ?isolation of pa