LeafGUSAssay
實驗概要a protocol for Leaf GUS Assay This protocol is for small samples (usually single leaf from 21DAI plants), scale up for larger samplesAs there are usually many samples, we use a microtitre plate and plate reader for quantifying the protein samples 主要試劑GUS Buffer (500ml) 2.0478g Na2HPO4 1.2688g NaH2PO4 (=50mM NaPi pH 7.0) &n......閱讀全文
Noble－Agar－Assay
DescriptionCancer cells do not show anchorage and contact inhibition of growth. To assess the anchorage and contact independent growth of cells, noble
LOWRY－PROTEIN－ASSAY
The Lowry procedure is one of the most venerable and widely-used protein assays, being first described in 1951 [Lowry et al.,?J. Biol. Chem. 193: 265-
Crystal－Violet－Assay
This is a simple assay useful for obtaining quantitative information about the relative density of cells adhering to multi-well cluster dishes. The dy
Migration－Assay－Protocol
Materials to be prepared beforehand:1) FBS free medium2) 10% FBS medium3) Cell migration filter insert ( Transwell?, 12mm Diameter, 12 μm Pore Size.)P
Adhesion－Assay－Protocol
Materials to be prepared beforehand:1) Washing Buffer--0.1% BSA in medium (DMEM or RPMI)2) Blocking Buffer--0.5% BSA in medium (DMEM or RPMI)3) Lamini
Cell－Viability－Assay
Dye exclusiona cell suspension is mixed with trypan blue and examined by low-power microscopyMaterialscellsPBSM3hemocytometer0.4 % trypan blue in PBSm
Leaf－GUS－Assay
一、實驗試劑 GUS Buffer (500 ml) 2.0478 g ? Na2HPO4 1.2688 g ? NaH2PO4 (=50 mM NaPi pH7.0) 10 ml ? ?0.5 M EDTA (=10 mM) 0.5 g ? ?Triton X-100 0.5 g ? ? N-L
Wound－healing－assay
The wound healing assay allows the researcher to study cell migration and cell interactions. In some cases also single cell migration can be analyzed.
Soft－Agar－Assay
Soft Agar AssayMake 0.6% media-agar mix for the bottom layer.??????? To make 0.6% agar mix the following components (this makes 200 ml):2X DME 100 mlI
MINICHROMOSOME－MICROTUBULE－BINDING－ASSAY
Determine the OD600?and correlate the cell density from the chart. Set up four 100mL YPD cultures at the following densities: 0.7x105, 1x105, and 3
Radioactive－DNA－Fragmentation－Assay
DESCRIPTION of the method: The DNA Fragmentation Assay allows to determine the amount of DNA that is degraded upon treatment of cells with certain
Enzyme－Kinetics－assay－of－the－WT
To assay 17 b-HSD activity in lysates, cells were harvested 48h after transfection using PBS Enzyme Free Cell Dissociation Solution ( specialty Media
Angiotensin－Protein－Kinase－Assay
James Hardwick's angiotensin assay protocol This specific procedure was developed to assay the activity of the Lck kinase expressed from a retroviral
GST－Activity－Fluorometric－Assay
實驗概要 The ?experiment provides a simple, fluorescence-based in vitro assay for ?detecting the GST activity using a fluorescence plate reader. The ass
Phosphate－Assay－by－Suprya－Jaydev
ReagentsAshing buffer:10 g Mg(NO3)2?100 ml EtOH1.5 N HCl stock:119.7 ml concentrated HCl (11.6M @ 36% by weight)880.3 ml H2O1 N Sulfuric acid stock:28
Cr－Release－Cytotoxicity－assay
DescriptionCytotoxic activities of mNK cells are examined in 51Cr release assay from target cells?ProcedureEffector cells (mNK cells) are seeded into
MTT－Cell－Proliferation－Assay
MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay, first described by Mosmann in 1983, is based on the ability of a mitochondri
Biorad－Protein－Assay:－Bradford
Biorad Protein Assay: BradfordStandards: 1 mg/ml BSA stock- dilute 1:10 to get 0.1 mg/ml BSAAdd To get H-2O20 μl 2 μg/ml 780 μl40 μl 4 μg/ml 760 μl60
Protocol－for－Aortic－Ring－Assay
ProceduresCover a 48-well plate with Matrigel (100μl/well) of and incubate for 30 min at 37?°C, 5% CO2.Sacrifice the1-2 month old mice/rats (WT/mutant
AlamarBlue?－Cell－Viability－Assay
實驗概要Assess cell viability.?實驗原理Cell ?health can be monitored by numerous methods. Plasma membrane integrity, ?DNA synthesis, DNA content, enzyme activ
Bradford－Protein－Concentration－Assay
Bradford Protein Concentration Assayversion 01/07/2001Abbreviations:mcg = microgramsmcL = microlitersBSA = bovine serum albuminO.D. = optical densityd
Cell－Clonogenic－Survival－Assay
DescriptionAllows one to test the capability of adherent cells to survive and replicate following insult with chemicals or radiation.?Procedure1. Grow
The－ribonuclease－protection－assay－(RPA)
The ribonuclease protection assay (RPA) is a highly sensitive and specific method for the detection of mRNA species. The assay was made possible by th
Hanging－drop－aggregation－assay
DescriptionThis assay is used for the aggregation property of cancer cells. It is a very critical parameter for measurement of cell metastasis. Factor
Xenograft－Tumor－Assay－Protocol
1) Determine the number of cells for injection (ie 5′106 ) to determine the number of plates thatwill require trypsinizing (usually a 100% confluent p
Alanine－Transaminase－Activity－Assay
實驗概要Alanine ?Transaminase (ALT) is a transaminase (EC 2.6.1.2) also called serum ?glutamic pyruvic transaminase (SGPT). Alanine Transaminase is found
Polyphenoloxidase－(catechol－oxidases)－assay
Browning of the cut surface of some fruits and vegetables is due the presence of a group of enzymes called polyphenoloxidases. These enzymes are relea
Nucleotide－Binding/Hydrolysis－Assay
MaterialsNucleotide mixMotor (50 - 100 μM; purity > 95%)0.5 M Tris-OAc, pH 7.510 mM EGTA10 mM MgCl2DDWSephadex G-50 Medium column (0.8 cm in x 20 cm)C
Chorioallantoic－Membrane－(CAM)－Assay
8 eggs per day, day 7- day 13?cut CAM, wash in precooled PBS,in 10 ml WASH 1 (PBS, 5 mM EDTA, COMPLETE) on icecut in pieces in petri dish on icecentri
In－Vivo－Ubiquitination－Assay－by－Agroinfiltration
The ubiquitination/proteasome system is involved in nearly all plant signaling processes. Many signaling components are degraded by the 26S protea