NobleAgarAssay
DescriptionCancer cells do not show anchorage and contact inhibition of growth. To assess the anchorage and contact independent growth of cells, noble agar assay can be utilized. Noble agar is used to coat the tissue culture plates to cover the polylysine coating of the plate. On this noble agar coated, anchorage independent tissue culture plates normal and cancer cells will be seeded in very low density to prevent t......閱讀全文
Noble Agar Assay
DescriptionCancer cells do not show anchorage and contact inhibition of growth. To assess the anchorage and contact independent growth of cells, noble
Soft Agar Assay
Soft Agar AssayMake 0.6% media-agar mix for the bottom layer.??????? To make 0.6% agar mix the following components (this makes 200 ml):2X DME 100 mlI
SOFT AGAR ASSAY FOR COLONY FORMATION
Note: All volumes are calculated to cater for four plates per point. Base Agar 1. Melt 1% Agar (DNA grade) in microwave, cool to 40 in a waterbath
Preparation of Agar plates
Prepare media and add 1.5 agar before autoclaving it (15g per liter).? After autoclavation, cool the media in a 55 degree waterbath. Do not allow? th
Plating in Top Agar
1. Warm plates to room temperature before use. Cold plates causes the top agar to solidify irregularly. DO not warm plates to 37° as the top agar will
Agar Plates for Selection of Clones in Bacteria
Cloning of PCR products Stocks: LB Agar: Luria Broth after Lennox: per Liter Tryptone 10 g Yeast Extract 5 g Sodium Chlori
幾種美國BD瓊脂粉的區別與技術參數對比
幾種美國BD瓊脂粉的區別與技術參數對比美國BD公司是世界上最大的生產和銷售醫療設備，醫療系統和試劑的醫療技術公司之一，該公司在生產的高品質微生物培養基及相關原料，在科研以及工業生產中被廣泛使用。針對同種產品，BD公司做的不同程度的細分。用戶在選用時有時比較困惑，該選擇那個貨號的產品更適用于實驗要求？
Preparing Antibiotics Stock Solution and Ampicillin Agar Plates
AMPICILLIN?Beta-lactam-antibiotics are not very stable when dissolved. Slow but steady degradation happens even when frozen to -20?°C. Therefore comme
Pectinase assay
Pectinases are actually a mixture of enzymes, which, along with others such as cellulase, are widely used in the fruit juice industry where they are
Protease assay
In certain fruits, such as pineapples and mangoes, the flesh contains protein-digesting enzymes (proteases). These may play a part in helping to soft