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實驗概要Plasmid DNA is isolated from large-scale (500 ml) bacterial cultures by treatment with alkali and SDS.主要試劑Buffers and SolutionsAlkaline lysis solution IFor preparations of plasmid DNA that are to be subjected to further purification by chromatography (please seeChapter 1, Protocol 9 ), sterile Alkaline lysis solution I may be supplemented just before use with the appropriatevolume of 20 mg/ml DNase-free RNase A (......閱讀全文
· Standard (alkaline lysis) Mini-Prep (Goldberg Lab)Standard protocol for mini-prep and reci
· Standard (alkaline lysis) Mini-Prep (Goldberg Lab)Standard protocol for mini-prep and reci
DNA Isolation by a Cleared Lysate Method Followed by Double Acetate Precipitation Version 3b - updated September 26, 1999The Most Recent Roe Lab Imple
Preparation of Cosmid DNAPreparation of Cosmid DNA from 50 ml Cultures (Donis Keller Lab)Cosmid vectors containing foreign DNA inserts are known
Preparation of Cosmid DNAPreparation of Cosmid DNA from 50 ml Cultures (Donis Keller Lab)Cosmid vectors containing foreign DNA inserts are known
Purification of plasmid DNA (miniprep) with high yields using diatomaceous earthKyung-Soo Kim and Charles K. PallaghySchool of Botany, La Trobe Univer
B. Midiprep double-stranded DNA isolationA midi-prep double-stranded DNA isolation has been developed to generate a sufficient amount of template DNA
Introduction The most important aspect of our cloning vectors is that t
III. Methods for DNA isolationA. Large scale double-stranded DNA isolationThe method used for the isolation of large scale cosmid and plasmid DNA is a
Application: Quantitative RT-PCR is used to quantify mRNA in both relative and absolute terms. It can be applied for the quantification of m
實驗概要The PureLink? Genomic DNA Purification Kit allows rapid and efficient purification of genomic DNA. The kit is designed to efficiently
Isolation of BAC DNA from Large-scale CulturesJoseph SambrookPeter Maccallum Cancer Institute and The University of Melbourne, AustraliaDavid W. Russe
實驗概要Plasmid DNA is isolated from small-scale (1-2 ml) bacterial cultures by treatment with alkali and SDS.主要試劑Buffers and Solutions: Alka
實驗概要The E.Z.N.A.? family of products is an innovative system that radically simplifies extraction and purification of nucleic acids from a
Small Scale Plasmid Isolation (Mini / Maxi Prep)Description A mini prep / maxi prep is used to isolate plasmid or cosmid DNA from bacteria, norma
Picking ES cell clonesOne or two days before picking colonies prepare 24-well plates of feeders. You can also use alternate protocols that utilize 96-
實驗概要 The immunoprecipitation (IP) of cross-linked chromatin with antibodies specific for certain histone modifications (chromatin &nb
Key points to observe: a. Use a endA1- E. coli strain for plasmid propagation and isolation whenever possible. The instabilit
Acknowledgements The organizer of the workshop acknowledges Dr. Murray Milford, Professor and Interim Head, and Dr. Mark Hussey, Professor and In
Fine Mapping of Genomic Targets of Nuclear Proteins in Cultured CellsAchim Breiling and Valerio OrlandoDulbecco Telethon Institute, Institute of Genet
Metabolic biotinylation of mammalian cell receptors for imagingBakhos A. Tannous , btannous@hms.harvard.edu, Massachusetts General Hospital and Harvar
2B. Transformation 1. Preparation of electrocompetent DH5a cells: autoclave 4 baffled 1 l
DNA轉化Chemical Transformation· Transformation of Competent Cells (RbCl2 Method) (Goldberg Lab
實驗概要The E.Z.N.A.TM Fastfilter Plasmid Midi Kit combines the power of HiBind? technology with the time-tested consistency of alkaline-SDS lysis of
實驗概要The E.Z.N.A.TM Fastfilter Plasmid Midi Kit combines the power of HiBind? technology with the time-tested consistency of alkaline-SDS lysis of
Large Scale Preps: (See Large scale plsasmid prep protocol for more details)Cultures: Inoculate a 5 mL LB/Amp (50 - 100 μg/mL) culture in early a
實驗概要Native chromatin immunoprecipitation to query specific chromatin states of individual genes. 主要試劑10 x TBS 0.1 M Tris-HCl (pH 7.5) 1.5 M NaCl
1. The preparation of native chromatin from cultured human cells1.1.Cultured cells (e.g. HL-60 or lymphoblastoids) are grown to a density of approxima
Preparing Lambda DNA1- Coliphage lambda DNA is a widely used vector for recombinant DNA. The middle third of its 48,000 bp contains no genes required
RNA提取（主要內容如下）Tips for Handing RNA Total RNA IsolationmRNA IsolationrRNA IsolationOthersQ & A posted in the Method Forum Basic Proc