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實驗概要 Western blot detection of histone proteins. 實驗步驟 The following protocol refers to the western blot detection of histone proteins derived from purified calf thymus performed at Abcam. 1. For each lane prepare 0.5 μg calf thymus or acid extracted histones diluted in 1X NuPAGE LDS sample buffer (Invitrogen) supplemented with 100 m......閱讀全文
應用：ChIP, ChIP-Seq, ELISA, IF, IHC, IP, WB克隆：多克隆是否帶有標記：無標記寄主：兔子同型對照：IgG純化：親和純化反應物種：可用物種廣泛，人，小鼠，大鼠濃度：1 mg/ml描述：兔多克隆抗體是針對K9三甲基化的組蛋白H3相應的合成肽，芯片級免疫原：一種人工合成
Packaging of DNA into chromatin allows the cell to store its genetic information efficiently and has an important role in regulating gene expression.
為什么選擇組蛋白H3為細胞核的內參指標呢？當實驗樣品中只是核蛋白，而不是細胞總蛋白提取液時，可以用組蛋白H（Histone H），或者增殖細胞核抗原（PCNA）等為核內參抗體。除了這些，其它常見的核蛋白內參還有K70， K80， Lamin A和B。但是需要注意的問題是核蛋白內參的選擇需要考
組蛋白（Histone）在真核生物染色體中扮演著重要的角色，是染色體結構單元核小體的重要組成部分。由核心組蛋白H3，H4，H2A，H2B形成的八聚體是DNA纏繞的主要承載體【1】。除了用以裝配染色體外，組蛋白的另外一個重要功能是參與基因組信息的表達調控。組蛋白氨基酸殘基上的翻譯后修飾如乙酰化、甲
The differentiation of muscle cells is transcriptionally regulated, in part by the myocyte enhancer factor-2, MEF2. During myogenesis MEF2 binds to My
實驗概要The method provides a procedure and tips for detecting histone proteins.The protocol refers to the western blot detection of histone p
PROTOCOLTo 1.5 mL eppendorf tubes add:200 μg of protein extract (see Western blot protocol for protein sample preps)q.s. to 300 μL with RIPA (with pro
大家好，我又來啦~~今天給大家放送的是表觀遺傳之組蛋白修飾相關的內容噢，組蛋白修飾也是一個比較復雜的過程，今天呢，我們就給大家講講組蛋白乙酰化及相關的產品。 一 組蛋白修飾 真核生物染色質的基本結構單位是核小體，它由約 146 bp DNA 纏繞組蛋白八聚體組成，其中組蛋白八聚體
選方案組蛋白去乙酰化酶（histone deacetylase,HDAC）是一類蛋白酶,對染色體的結構修飾和基因表達調控發揮著重要的作用。一般情況下,組蛋白的乙酰化有利于DNA與組蛋白八聚體的解離，核小體結構松弛，從而使各種轉錄因子和協同轉錄因子能與DNA結合位點特異性結合，激活基因的轉錄。在細胞核
The vitamin D receptor, VDR is the mediator of all genomic actions of vitamin D3 and its analogs. It belongs to a family of ligand induced transcripti
METHOD Analysis of precipitated chromatin fractions (from Chromatin Immunoprecipitation on Unfixed Chromatin from Cells and Tissues to Analy
組蛋白去乙酰化酶（histone deacetylase,HDAC）是一類蛋白酶,對染色體的結構修飾和基因表達調控發揮著重要的作用。一般情況下,組蛋白的乙酰化有利于DNA與組蛋白八聚體的解離，核小體結構松弛，從而使各種轉錄因子和協同轉錄因子能與DNA結合位點特異性結合，激活基因的轉錄。在細胞核內
實驗概要This protocol describes how chromatin is prepared from Arabidopsis, which can subsequently be used for chromatin immunoprecipitation (ChIP). T
INTRODUCTION After chromatin immunoprecipitation (ChIP), different PCR-based approaches can be used to determine how much DNA is p
實驗概要 The immunoprecipitation (IP) of cross-linked chromatin with antibodies specific for certain histone modifications (chromatin &nb
The packaging of eukaryotic DNA into nucleosomes inhibits the access of factors to DNA and results in the repression of transcription, replication and
Huntington's disease is a neurodegenerative condition caused by a dominant mutation in a gene encoding a protein now called huntingtin. Large poly
INTRODUCTIONAfter chromatin immunoprecipitation (ChIP), different PCR-based approaches can be used to determine how much DNA is precipitated at a locu
INTRODUCTIONAfter chromatin immunoprecipitation (ChIP), different PCR-based approaches can be used to determine how much DNA is precipitated at a locu
Several forms of post-translational modification regulate protein activities. Recently, protein methylation by CARM1 (coactivator-associated arginine
組蛋白乙酰化修飾是基因表觀轉錄調控的重要機制.組蛋白翻譯后修飾所引起的染色質結構重塑在真核生物基因表達調控中發揮著重要的作用.組蛋白乙酰化主要發生在H3、H4的N端比較保守的賴氨酸位置上，是由組蛋白乙酰轉移酶和組蛋白去乙酰化酶協調進行。組蛋白乙酰化呈多樣性，核小體上有多個位點可提供乙酰化位點，但特定
Tumorigenesis is known to be a multistep process in which defects in various cancer genes accumulate. Epigenetic modifications, most importantly DNA m
The chromatin packaging of the genome is dynamic, changing with the cell cycle and with transcriptional regulation. During mitosis, chromatin is conde
Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College Exercise 10.3 - Extraction of ChromatinLEVEL IIMaterials Bovine
組蛋白去乙酰化酶（HDAC, histone deacetylase）是癌癥藥物研發領域的熱點。在人類中存在18種不同的HDAC，可以進一步細分為4類：I類HDAC（HDAC1、2、3和8）主要存在于細胞核中，主要功能是給組蛋白脫乙酰基。II類HDAC進一步分為：IIa類（HDAC4、5、7和9
DNA是攜帶生物體遺傳信息的重要分子，它的完整性對細胞存活至關重要。然而，在生命活動中，DNA時刻遭受著內源性（氧化自由基、復制叉崩塌等）或外源性（電離輻射、烷化劑等）刺激，DNA損傷不可避免。檢測DNA損傷的方法有很多，根據其原理大致可以分為3類： 基于損傷DNA理化性質的改變檢測DNA
Several forms of post-translational modification regulate protein activities. Recently, protein methylation by CARM1 (coactivator-associated arginine
在一項新的研究中，來自英國利物浦大學和鄧迪大學的研究人員針對細胞如何應對缺氧提出了新見解。他們發現作為DNA和蛋白的復合物，染色質在低氧條件下快速地發生變化。相關研究結果發表在2019年3月15日的Science期刊上，論文標題為“Hypoxia induces rapid changes to
增殖細胞核抗原（Proliferating Cell Nuclear Antigen簡稱PCNA）由Miyachi等于1978年在 SLE（系統性紅斑狼瘡）患者的血清中首次發現并命名，因其只存在于正常增殖細胞及腫瘤細胞內而得名。PCNA是一種分子量為36KD的蛋白質，在細胞核內合成，并存在于
摘要 在腫瘤學研究及治療過程中，監控細胞周期及凋亡的狀態是一個非常重要的指標。 與傳統的檢測方法相比，如流式細胞FACS，全自動高內涵成像分析系統可對原位（無需消化懸浮處理）細胞進行全自動成像，并完成單個細胞水平的多參數分析。MetaXpressTM圖像分析及獲取軟件具有一系列常