Detectionofapoptoticprocessinsituusingimmunocytochemical
1. INTRODUCTION Apoptosis was observed from invertebrates to lower and higher verterbrates, and intervenes both in physiological and in pathological states, such as normal cell turnover, embryogenesis, metamorphosis, thymic maturation and involution, neoplasm, etc. (1-4).Endonucleolysis is considered as the key biochemical event of apoptosis, resulting in cleavage of nuclear DNA into oligonucleosome-sized ......閱讀全文
Detection－of－apoptotic－process－in－situ－using－immunocytochemical
1. INTRODUCTION??Apoptosis was observed from invertebrates to lower and higher verterbrates, and intervenes both in physiological and in pathological
Detection－of－apoptotic－process－in－situ－using－immunocytochemical2
B) TUNEL in situ procedureB.2.1 Materialsproteinase K (pK) (A2), H2O2?, TdT buffer (A1), TdT enzyme (A2), biotinylated dUTP (A2), TB buffer (A1), seru
In－Situ－Cell－Death－(Apoptosis)－Detection－by－TUNEL－labeling
Protocol for Frozen Sections:Warm 150ml 4% Paraformaldehyde/1x PBS to RT. Fix slides in it, 20 min., RT.1x PBS rinse, 2 times.1x PBS, 30 min., RT. Beg
Guide－to－Cell－Proliferation－and－Apoptosis－Methods
Chapter 1: Cell Death - Apoptosis and Necrosis 1.1 Introduction 2 1.1.1 Terminology of cell death 2 1.1.2 Differences between necros
Detection－by－TUNEL－labeling
In Situ Cell Death (Apoptosis) Detection by TUNEL labelingby Boehringer Mannheim (Catalog No. 1684809), modified by Josiah N. Wilcox,José C. Rodriguez
Fluorescence－In－Situ－Hybridization－using－TSA?
實驗概要This ?protocol describes steps for fluorescent in situ hybridization (FISH) ?to Drosophila embryos using Tyramide Signal Amplification (TSA?), and
凋亡細胞核DNA片段檢測方法進展（二）
2.3 凋亡細胞的TUNEL和ISNT鑒定的流式細胞儀分析[16]對于培養的細胞，可以將TUNEL或ISNT鑒定同流式細胞儀結合起來分析其發生凋亡的情況。待檢細胞與含有TdT或DNA聚合酶I或Klenow片段及生物素標記的dUTP反應液共孵育一段時間后，加入熒光素（常用FITC）標記的鏈霉抗生物
ASENSITIVE－METHOD－FOR－DETECTION－OF－APOPTOSIS－BY－SINGLE－LASER－FLOW－CYTOMETRY
MATERIALS:1. 1 X PBS (PBSAz, 1 X PBS, e.g., Irvine Scientific, CA, containing 2% newborn calf serum and 0.1% sodium azide)2. 7-Amino-actinomycin D (7-
Detection－of－MicroRNA－Heterogeneity－in－Single－Cells－Using－an－Automated
Introduction ?MicroRNA ?(miRNAs) are short (18–24 nucleotides), non-coding RNAs that regulate ?gene expression by both disrupting messenger RNA (mRNA
碳水化合物分析
Carbohydrate Assay?(Hancock Laboratory) (Accessible only by IE) This protocol is used to determine the relative amounts of LPS CHO present in a giv
細胞周期的流式細胞伩檢測實驗方法（PI，Brdu)2
B.3. COMMENTARY?B.3.1 Background information?The critical steps in the methodology are cell fixation, permeabilization and the concentrations of anti-
Detection－of－Viruses－in－Infected－Plant－Extracts－using－ImmunocapturePCR
?1) Immunocapture stage Coating buffer: 15 mM Na2CO3; 35 mM NaHCO3, and 3 mM NaN3, per liter (pH 9.6). Extraction buffer: (20 mM Tris-HCL (pH 8
Detection－of－Viruses－in－Infected－Plant－Extracts－using－ImmunocapturePCR
?1) Immunocapture stage Coating buffer: 15 mM Na2CO3; 35 mM NaHCO3, and 3 mM NaN3, per liter (pH 9.6). Extraction buffer: (20 mM Tris-HCL (pH 8
Alexa－Fluor?－488－Annexin－V/Dead－Cell－Apoptosis－Kit
實驗概要Apoptosis is a ?carefully regulated process of cell death that occurs as a normal part ?of development. Inappropriately regulated apoptosis is imp
TUNEL－PROCEDURE－IN－BOVINE－EMBRYOS
Materials 8% (w/v) paraformaldehyde stock solution:??Dissolve 8 g of powdered paraformaldehyde in 100 ml water. Heat and stir (55-60 C – do not go h
TUNEL－PROCEDURE－IN－BOVINE－EMBRYOS
Materials 8% (w/v) paraformaldehyde stock solution:??Dissolve 8 g of powdered paraformaldehyde in 100 ml water. Heat and stir (55-60 C – do not go h
Tunel－Procedure－in－Bovine－Embryos－牛胚胎TUNEL檢測凋亡
Materials8% (w/v) paraformaldehyde stock solution:?Dissolve 8 g of powdered paraformaldehyde in 100 ml water. Heat and stir (55-60 C – do not go highe
流式細胞術定量檢測細胞凋亡的3種方法研究比較
細胞凋亡的定性檢測依賴于形態學觀察及DNA電泳,其定量檢測則需借助于流式細胞檢查。使用碘化丙啶(PI)染色檢測DNA含量是最早出現的凋亡定量檢測方法［1］。進入90年代,檢測DNA斷裂點的TUNEL(Terminal deoxylnucleotidyl transferase mediated-dU
流式細胞術定量檢測細胞凋亡3種方法的比較研究
細胞凋亡的定性檢測依賴于形態學觀察及DNA電泳,其定量檢測則需借助于流式細胞檢查。使用碘化丙啶(PI)染色檢測DNA含量是最早出現的凋亡定量檢測方法［1］。進入90年代,檢測DNA斷裂點的TUNEL(Terminal deoxylnucleotidyl transferase mediated-dU
A－rapid,－quantitative－and－inexpensive－method－for－detecting－apoptosis2
Figure?1.Determination of apoptosis in transiently transfected murine [beta] tumor cells. (A) The number of apoptotic [beta]HC 13T tumor cells (% apop
Silver－Acetate－Autometallography－(AMG)
In the early eighties, a series of papers were published by Gorm DANSCHER, Aarhus, Denmark, to introduce a reliable and easy-to-handle technique for
Apoptosis:－A－Laboratory－Manual－of－Experimental－Methods－Andrea－Cossarizza
THE CELL?1.?Morphological aspects of apoptosis?Walter Malorni, Stefano Fais & Carla Fiorentini?2.?Cell cycle?Miriam Capri & Daniela BarbieriTHE NUCLEU
BrdU－Labeling－Protocol
實驗概要The thymidine analog, 5-bromo-2-deoxyuridine (BrdU),is a common reagent used for cell proliferation assays and for the detection of apoptotic
實驗室自動化與篩選協會2013亞洲會展展商技術論壇
第一天(2013年6月6日)13:45-14:30 展商技術論壇 1： 會場：宴賓廳 II，2樓 議題：Cell-based assays and 3D cell culture：Your body is in 3D – Why is your cell cult
細胞遺傳學——原位雜交(ISH)
In Situ Hybridization· ????????In Situ Hybridization?(jsmith1@po-box.mcgill.ca)In situ?hybridization, as the name suggests, is a method of localizing,
細胞周期的流式細胞伩檢測實驗方法（PI，Brdu)1
ANALYSIS OF CELL CYCLE?Miriam Capri and Daniela Barbieri?Dept. Biomedical Sciences, Sect. General Pathology,Via Campi, 287, University of Modena, 4110
FACS－Procedures－for－Apoptosis－Detection
Materials:Hoechst?33258?(Sigma B-2883).stock: 10 mg/ml in dH20 (40)working dilution: 500μg/ml (50μl stock + 950μl PBS).7-Amino-actinomycin (Sigma A-94
Methods－for－the－Measurement－of－a－Bacterial－Enzyme－Activity－in－Cell－Lysates4
The use of a micro-titre based colorimetric assay provided a third method for the study of ACTase activity, and the most useful for large scale measu
流式細胞術檢測細胞凋亡
實驗概要Provides a rapid and convenient assay for apoptosis.?實驗原理Apoptosis ?is a carefully regulated process of cell death that occurs as a normal ?part o
六種染色后光鏡觀察法檢測肝癌細胞凋亡
作者：楊連君，司曉輝，王文亮，王文勇，趙一嶺，方正清［摘? 要］ 目的：探討簡便易行的在光鏡下通過形態學觀察檢測細胞凋亡的方法，并對其進行比較。方法：首先用6%的乙醇作用6 h誘導人肝細胞癌細胞系HCC9204細胞凋亡，然后進行未固定細胞的臺盼藍染色、吖啶橙/溴化乙啶(AO/EB)雙染色，細胞固定后