DNA and RNA Staining6. Stain cells with 7-AAD: i. Resuspend the cells from Step 5 in 0.5 mL of NASS containing 10 μg/mL of 7-AAD. Incubatefor 20 min at 20°C-25°C, protected from light. ii. Prepare a cell sample that is stained only with 7-AAD (without any cell surface staining). This sample is necessary to determine the amount of fluorescence overlap of 7-AA......閱讀全文
Figure 2 – Jablonski diagram showing transition of energy for Rayleigh and Raman scattering.Scattered light itself can be distinguished between
Homogeneity of cell populations is a prerequisite for the analysis of biochemical and molecular events during male gamete differentiation. Given the c
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October 22, 2015 - Giesen, GermanyWant to Prevent Costly Label-Mix-Up and Significantly Reduce Product Giveaway in Your Line?Easy task for the world
Flow cell assays are very useful for studying microtubule motility, microtubule dynamics, kinetochore-microtubule interactions and action of severing/
Enzyme-linked Immunosorbent Assays (ELISAs) combine the specificity of antibodies with the sensitivity of simple enzyme assays, by using antibodies or
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The single most important advance in the use of centrifugal force to separate biologically important substances. was the coupling of mechanics, optics
3.6 Key references1. Kroemer G., Zamzani N., Susin S.A. Mitochondrial control of apoptosis. Immunol. Today, 18: 44-51, 1997.2. Susin S.A., Z
2 Materials2.1 Labeling1. 2′,3′-O-(2-Aminoethyl-carbamoyl)-adenosine-5′-triphosphate (edaATP) (Jena Biosciences) (see Not
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Histologic analysis of murine BM is a necessary complement to flow cytometric or in vitro analysis. Techniques to do this are well established in
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Methods Materials. A uniform mixture of six standard glycoproteins was used as the starting material, including IgG (56834, catalog numeber),
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The plasticity of differentiated adult cells could have a great therapeutic potential, but at the same time, it is characteristic of progression of se
51. Krupenina NA, Bulychev AA. Action potential-induced changes in photosynthetic pattern of a plant cell