FluorescentNucleosideTriphosphatesforSingleMoleculeEnzymology3
2 Materials2.1 Labeling1. 2′,3′-O-(2-Aminoethyl-carbamoyl)-adenosine-5′-triphosphate (edaATP) (Jena Biosciences) (see Note 1).2. 3′-Amino-3′-deoxyATP triethylammonium salt (aminoATP) (15).3. Cy3 N-hydroxysuccinimide ester (NHS-ester) (GE Healthcare).4. 7-Diethylaminocoumarin-3-carboxylic acid (Invitrogen).5. 20 mM Sodium bicarbonate, pH 8.4.6. Dimethylfo......閱讀全文
Fluorescent Nucleoside Triphosphates for Single-Molecule Enzymology-5
References 1. Funatsu, T., Harada, Y., Tokunaga, M., Saito, K., and Yanagida, T. (1995) Imaging of single fluorescent molecules and individual A
Fluorescent Nucleoside Triphosphates for Single-Molecule Enzymology-2
1.1 ??Selection of the Labeling PositionFluorescent adenine and guanine nucleotides have been widely used to report upon binding, protein release and
Fluorescent Nucleoside Triphosphates for Single-Molecule Enzymology-1
By:?Christopher P. Toseland1 2?,?Martin R. Webb1Affiliation(s):?(1)?MRC National Institute for Medical Research, London, UK(2)?Institut für Zellul?re
Fluorescent Nucleoside Triphosphates for Single-Molecule Enzymology-4
3.2.1 ??LabelingThis method requires the starting material 3′-amino-3′-deoxy--ATP (15).1.?Activate 7-diethylaminocoumarin-3-carboxylic acid (16.4 mg,
Fluorescent Nucleoside Triphosphates for Single-Molecule Enzymology-3
2 ??Materials2.1 ??Labeling1.?2′,3′-O-(2-Aminoethyl-carbamoyl)-adenosine-5′-triphosphate (edaATP) (Jena Biosciences) (see Note?1).2.?3′-Amino-3′-deoxy
NUDT1基因編碼功能及結構描述
在復制和轉錄過程中，氧化核苷三磷酸錯合成DNA / RNA可引起突變，這可能導致癌變或神經變性。由該基因編碼的蛋白質是酶水解氧化嘌呤核苷三磷酸鹽，例如8-氧代-DGTP，8-氧代-DATP，2-羥基-DATP，和2-羥基-RATP，對單磷酸，從而防止錯誤摻入。編碼的蛋白質主要定位在細胞質中，有些位于
NUDT1基因突變與藥物因子介紹
在復制和轉錄過程中，氧化核苷三磷酸錯合成DNA / RNA可引起突變，這可能導致癌變或神經變性。由該基因編碼的蛋白質是酶水解氧化嘌呤核苷三磷酸鹽，例如8-氧代-DGTP，8-氧代-DATP，2-羥基-DATP，和2-羥基-RATP，對單磷酸，從而防止錯誤摻入。編碼的蛋白質主要定位在細胞質中，有些位于
Fluorescent Staining of Cells
1. Fluorescent phalloidin in methanol. Phallacidin does not work as well. Dilute 10 ul 330 nM stock into 500 ul PBS for each large coverslip. 2.
Measurement of Green Fluorescent Protein Expression
? Reagents Cells to be studied expressing green fluorescent protein (GFP). Note that the same cell type without GFP is needed as control. Hoechst
Basic Fluorescent in situ Hybridization (FISH)
實驗概要 Fluorescence ?in situ hybridization method is a kind of physical map drawing method, ?use fluorescent element mark probe, to detect probe and s