IntracellularImmunofluorescentStainingforFlowCytometry
IntroductionA modification of the basic immunofluorescent staining and flow cytometric analysis protocol can be used for the simultaneous analysis of surface molecules and intracellular antigens at the single-cell level. In this protocol, cells are first activated in vitro, stained for surface antigens as in the surface antigen protocol, then fixed with paraformaldehyde to stabilize the cell membrane a......閱讀全文
Intracellular Immunofluorescent Staining for Flow Cytometry
IntroductionA modification of the basic immunofluorescent staining and?flow cytometric analysis protocol?can be used for the simultaneous analysis of
Intracellular Immunofluorescent Staining for Flow Cytometry-2
Table 2: Human Cytokines: Intracellular Staining Quick GuideHuman Cytokines: Intracellular Staining Quick GuideHuman CytokineCell SourceActivationIncu
Detection of Intracellular Antigens by Flow Cytometry
實驗概要Fix and Perm ?reagents are designed for use with all commercially available flow ?cytometers. Alignment and compensation should be performed accor
Intracellular Staining Protocol
1.?Fix cells- Add16% formaldehyde directly into culture medium to obtain a final concentration of 1.5% formaldehyde. 2. Incubate in fixative for 10
Intracellular Cytokine Staining Protocol
實驗概要A ?modification of the basic immunofluorescence staining and flow ?cytometric analysis protocol can be used for the simultaneous analysis ?of surf
Flow Cytometry Analysis
PurposeFlow cytometry employs instrumentation that scans single cells flowing past excitation sources in a liquid medium. The technology can provide r
Yeast Cell Cycle by Flow Cytometry
ReagentsCold absolute ethanol.0.5 M Na citrate stock (filtered), 50mM diluted stock.10 mg/ml RNase A (Boil 10 mins, cool, filter and store at -20°C).4
流式細胞儀技術專輯
Flow Cytometry Analysis?(Springer Lab, Harvard University)? Flow cytometry employs instrumentation that scans single cells flowing past excitation
流式細胞儀技術專輯
?最方便的實驗干貨查詢工具微信掃碼進入「丁香實驗」小程序編輯：?嗚咽分享到：??????Flow Cytometry Analysis?(Springer Lab, Harvard University)Flow cytometry employs instrumentation that scan
Flow Cytometry of Fibroblast Nuclei for DNA content
Materials P.I. Solution:? 4 mM Na3Citrate (0.118 g/100 mL) 30 U/mL RNAseI (43 mg/100 mL) 0.1% Triton-X100 (0.1mL/100 mL) 50 μg/mL propidium iodide