MinichromosomeMicrotubuleBindingAssay微染色體－微管結合實驗2
YWB per 10ml5mL 2M Sorbitol (if NZ arrested, add 40uL 1.5mg/mL0.336mL 1M K2HPO4 N2 to 4mL YWB)0.064mL 1M KH2PO44.6mL dH2OYWB, glycerol, PMSF5mL 2M Sorbitol0.336mL 1M K2HPO40.064mL 1M KH2PO40.5mL 100% ultrapure glycerol0.05mL 0.2M PMSF4.05mL dH2O1X EBB, glycerol, PMSF (5mL)1mL 5X EBB0.25mL 100% glycerol12.5uL 0.2M PMSF3.75uL dH2O1X EBB, glycerol, DTT, BSA (10mL)2mL 5X EBB0.5mL glycerol10uL 100mg/mL BSA10uL 0.1M D......閱讀全文
Glycolipid Binding Assay
Glycolipid Binding Assay Source:?Contributed by Pingsunjim, Paller’s Lab Abstract:?This protocol can be used for the detection of gly
MINICHROMOSOME MICROTUBULE BINDING ASSAY
Determine the OD600?and correlate the cell density from the chart. Set up four 100mL YPD cultures at the following densities: 0.7x105, 1x105, and 3
Nucleotide Binding/Hydrolysis Assay
Materials Nucleotide mix Motor (50 - 100 μM; purity > 95%) 0.5 M Tris-OAc, pH 7.5 10 mM EGTA 10 mM MgCl2 DDW
MINICHROMOSOME MICROTUBULE BINDING ASSAY-2
HYBRIDIZATION. Prehybridize blot at 65oC for ~3h in Church buffer containing 0.5mg/ml denature salmon sperm DNA (usually 14ml Church buffer plus
Minichromosome-Microtubule Binding Assay 微染色體－微管結合實驗1
Koshland Lab,Carnegie Institute?http://www.ciwemb.edu/labs/koshland/Protocols/MICROTUBULE/mmb.htmlDetermine the OD600?and correlate the cell density f
Minichromosome-Microtubule Binding Assay 微染色體－微管結合實驗2
YWB per 10ml5mL 2M Sorbitol (if NZ arrested, add 40uL 1.5mg/mL0.336mL 1M K2HPO4?N2 to 4mL YWB)0.064mL 1M KH2PO44.6mL dH2OYWB, glycerol, PMSF5mL 2M Sor
Penicillan Binding Protein Assay青霉素與細胞膜蛋白結合實驗
Wash cells with 10 mM Tris pH 8French pressSlow speed spinHigh speed spinResuspend in 10 mM TrisSonicate 2 x 15 sec to remove ?-lacatamasesWash in 10
Microtubule Binding Assays
MaterialsSiliconized ultracentrifuge microfuge tubesGTP-depleted microtubules6X SDS loading dye1X SDS loading dyeCoomassie Brilliant Blue R 250 (0.8%
Pectinase assay
Pectinases are actually a mixture of enzymes, which, along with others such as cellulase, are widely used in the fruit juice industry where they are
Protease assay
In certain fruits, such as pineapples and mangoes, the flesh contains protein-digesting enzymes (proteases). These may play a part in helping to soft