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Characteristics of this procedure:Cells are grown to 80% confluency, then harvested, washed and disrupted in KPM buffer by freezing-thawing cycles with liquid nitrogen essentially as described by Fearnhead et al., 1997, Genes Dev. 11: 1266-76.PROCEDUREGrow cells in a 160 cm2 flask (25 ml medium) up to about 80% cell density.Remove the RPMI medium and detach cells by incubating with 10 ml trypsin/EDTA (Sigma) for......閱讀全文
IntroductionIn our lab we use the term 'cell-free system' when we talk about the examination of apoptotic activity in cytoplasmic extrac
Application: Quantitative RT-PCR is used to quantify mRNA in both relative and absolute terms. It can be applied for the quantification of m
AbstractThe kinetic characteristics and regulation of aspartate carbamoyltransferase activity were studied in lysates and cell extracts of Helico
實驗概要Primary tissues are valuable tools for the study of intracellular and extracellular markers which characterize disease states. We have
實驗概要Preparation of lysis buffers, protease and phosphatase inhibitors, lysate from cell culture, lysate from tissues, protein concentratio
中國科學院大連化學物理研究所 許國旺研究員 來自中國科學院大連化學物理研究所的許國旺研究員做了題為“液相色譜-質譜用于代謝標志物的發現和鑒定”的報告。許老師提出，現在是一個組學的時代，代謝組學作為最主要的組學之一，目前在疾病研究、藥物研發及職務和微生物等領域均得到重視。 代謝組學是研
2 kinds of newly launched products are available to SLAS Exhibitors to distribute on 2013 SLAS Asia Conference & Exhibition website. This
I. INTRODUCTION 引言 This guidance helps sponsors of investigational new drug applications (INDs) or applicants of new drug applications (NDAs),
南方科技大學前沿與交叉科學研究院（以下簡稱南科大交叉研究院）致力于促進前沿科學的探索研究與學科發展的交叉融合。通過組織不同學科和專業背景的科研人員開展協同合作研究，創新體制機制，聚焦重大科研方向，跨越學科邊界，實現學科間的思維碰撞與技術共享，積極地促進南方科技大學的前沿科學發展、學科交叉融合和先進技
Figure 1: epMotion 5073l worktable for qPCR setup Results and DiscussionThe automation of the qPCR setup using the 10 μL dispensing tool a
基于epMotion 5075t系統與KPPA HyperPlus試劑盒的全自動測序前文庫制備方案Automated KAPA HyperPlus DNA Library Preparation for Illumina? Sequencing on the Eppendorf epMot
尊敬的各位同行： 第2 屆大連國際色譜學術報告會及儀器展覽會包含第37屆國際高效液相色譜及相關技術會議(含儀器展覽會) (HPLC 2011 Dalian) 及第18屆全國色譜學術報告會及儀器展覽會(18th NSEC)，將于2011年10月8-11日在大連召開。 為使更多色譜工作者
如何借助epMotion 5073l移液工作站完成微量體積的qPCR反應體系構建在進行微量體系的液體操作時，如何避免人為誤差與日間變化，如何保證結果的一致性提高實驗效率？如果減少冗長枯燥的重復操作，減少昂貴試劑的使用，提升操作體驗節省實驗經費？本篇應用旨在介紹如何用10 μL分液工具實現全
INTRODUCTIONThe regulations promulgated to implement the amended Animal Welfare Act require that all survival surgery be performed using aseptic proce
4. 總結及展望SIET在借鑒眾多科學家工作經驗的基礎上，經過多年的改進和完善，為科研人員提供了一個較為友好的軟硬件環境，在數據的生成，采集以及校準等方面，極大地方便了研究人員。特別是應用SIET強大的3維立體測量方式，研究人員可以獲得其他電生理技術無法測到的被測樣品某些點的特異活性 [10]。對于
For ionisation to take place at all, chemical reaction between the sample and the&nbs
實驗概要The procedure presented below describes a facile method for studying signal transduction events with suspension cells (Jurkat, Raji, T
實驗概要The procedure presented below describes a facile method for studying signal transduction events with suspension cells (Jurkat, Raji, T
實驗概要The procedure presented below describes a facile method for studying signal transduction events with suspension cells (Jurkat, Raji, T
Introduction Multi-cellular populations are fundamentally driven by the collective properties of individual cells. However, our unde
利用Mastercycler X50 PCR儀與epMotion系統實現高重復性的微量體積PCR實驗Highly reproducible low Volume PCR with Mastercycler? X50 and epMotion? Arora Phang, Tim Schommartz,
實驗概要The procedure presented below describes a facile method for studying signal transduction events with adherent cells (HeLa, MCF-7, BALB
實驗概要The procedure presented below describes a facile method for studying signal transduction events with adherent cells (HeLa, MCF-7, BALB
Purification of plasmid DNA (miniprep) with high yields using diatomaceous earthKyung-Soo Kim and Charles K. PallaghySchool of Botany, La Trobe Univer
實驗概要Preparation of lysis buffers, protease and phosphatase inhibitors, lysate from cell culture, lysate from tissues, protein concentratio
RNA提取（主要內容如下）Tips for Handing RNA Total RNA IsolationmRNA IsolationrRNA IsolationOthersQ & A posted in the Method Forum Basic Proc
蛋白質組數據處理暨第三屆全國生物質譜學術交流會(第三輪通知) 為了積極促進我國蛋白質組學技術發展和應用、數據挖掘和生物質譜的經驗交流，由中國生物化學與分子生物學會蛋白質組學專業委員會、中國質譜學會生物質譜專業委員會和中國化學會分析化學委員會主辦，北京蛋白質組研究中心、復旦大學和蛋
Instruments For ElectrophysiologyProducts include microelectrode, voltage and current clamp amplifiers, perfusion chambers, perfusion heating systems,
INTRODUCTIONThe regulations promulgated to implement the amended Animal Welfare Act require that all survival surgery be performed using aseptic proce
賽默飛世爾科技推出完整LC-MS/MS解決方案用于環境和飲用水分析 2007年3月23日，圣何塞，加州，賽默飛世爾科技，服務科學的業界領袖，宣布推出一套完整的LC-MS/MS解決方案，在環境和飲用水分析中，可以顯著減少分析時間和樣本處理成本。EQuan 環境定量系統以賽默飛世爾科技的TSQ Q