CultureofHumanEmbryonicStemCells(hESC)
All cell lines are initially grown according to the supplier's protocols but we are adapting them to one simple protocol outlined below:6-well plates (Falcon Cat #353046) are coated for 20 to 60 minutes at room temperature with 0.1% gelatin (Sigma Cat #G1890) in dH2O.Mouse embryonic fibroblasts (CF1 strain), cultured in MEF medium, are mitotically inactivated by treatment with 10μg/ml mitomycin C (Roche Cat ......閱讀全文
Culture of Human Embryonic Stem Cells (hESC)
All cell lines are initially grown according to the supplier's protocols but we are adapting them to one simple protocol outlined below: 6-well pl
Isolation and Culture of Human Liver Stem Cells
Materials and Methods 1.?Human hepatocytes were isolated from fresh surgical specimens of patients undergoing hepatectomies. Healthy liver tiss
Noninvasive Human Nuclear Transfer with Embryonic Stem Cells
Noninvasive Human Nuclear Transfer with Embryonic Stem Cells Sohyun L. McElroy1 ?and? Renee A. Reijo Pera Center for Human Embryonic Stem Cell Re
Human Embryonic Stem (ES) Cell Protocols——Freezing Human ES Cells
? Collagenase cells for approximately 7 minutes at 37 °C (until edges of colonies are curling up). With a 5 ml pipet, gently pipet and scrape colon
Human Embryonic Stem (ES) Cell Protocols——Thawing Human ES cells
Remove Human ES cells from liquid nitrogen storage tank. Fill out a freeze/thaw form. Thaw cryovial by gently swirling in waterbath until only a smal
Derivation of Dopaminergic Neurons (from Human Embryonic Stem Cells)
實驗概要Directed ?differentiation of specific lineages has been a focal point in the ?field of human embryonic stem cell (hESC) research. Cell replacement
Human Embryonic Stem (ES) Cell Protocols——Splitting Human ES cells on MEFs
based on splitting onto one plate Warm collagenase IV split media to 37 °C in a water bath. Aspirate media off of cell culture plate. Add the follow
Human Embryonic Stem (ES) Cell Protocols—Splitting Human ES cells onMatrige
based on splitting onto on plateWarm collagenase media to 37°C in a water bath.Aspirate media off of cell culture plate.Add the following amount of co
Culturing BG01V Human Embryonic Stem Cells with Mouse Embryonic Fibroblast
If culturing in the absence of a feeder cell layer is desired, human embryonic stem (hES) cells can be maintained using Mouse or Human-Conditioned Med
Procedure for Culturing BG01V Human Embryonic Stem Cells
IntroductionHuman embryonic stem (hES) cells are pluripotent stem cells derived from pre-implantation embryos that can be maintained and expanded in a