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In certain fruits, such as pineapples and mangoes, the flesh contains protein-digesting enzymes (proteases). These may play a part in helping to soften the fruit tissues as the fruit ripens, making it even more attractive to animals that might disperse the seeds. So perhaps the activities of these proteases enzymes will increase during the ripening process.Applications of plant proteases, such as ‘bromelain’ from the......閱讀全文
Chloroplasts are metabolically important organelles that perform many essential functions within plant cells. The chloroplasts can be subdivided i
實驗概要We provide a general IP procedure including a list of reagents and a table to help you choose the correct protein beads.Immunoprecipit
Table 1: Hydrolysis of 125I-labeled Ub-PESTc by the purified YUH1.Specific activity againstDUBsCbz-LRGG-AMC125I-labeled Ub-PESTcYUH13.2 x 10-105.
實驗概要 In certain fruits, such as pineapples and mangoes, the flesh contains protein-digesting enzymes (proteases). These
The plasticity of differentiated adult cells could have a great therapeutic potential, but at the same time, it is characteristic of progression of se
借助Chromotek公司GFP-Trap如何設計成功的IP實驗？How to plan an immunoprecipitation of your GFP-fusion protein when using the ChromoTek GFP-Trap?PreambleThis document
實驗概要This protocol describes how chromatin is prepared from tissue, which can subsequently be used for chromatin immunoprecipitation (ChIP)
Metabolic biotinylation of mammalian cell receptors for imagingBakhos A. Tannous , btannous@hms.harvard.edu, Massachusetts General Hospital and Harvar
COMPARISION OF DIFFERENT PROTEIN DETERMINATION METHODSCompanyMethodDetection RangeApplications -CompatibilityAssay protocolPrecautions
MTT Assay on Reattached CellsAs seen in Fig. 2 , the proportion of viable MSC that re-attached was significantly higher (p?
Bovine Ig(Total Immunoglobulin) ELISA KitCatalogue No: EB0001 Size: 48T/96T Reactivity: BovineDetection Range: 1.563-100ug/mlSensitivity: <0.938ug/
為了進一步提高檢測基因的效率，我們對螢光素酶基因序列的密碼子進行了優化，使得它在多種哺乳細胞中的表達水平提高了5～10倍；同時，為了減少對基因的非特異性調控，我們也對螢光素酶的載體進行了優化，去除了載體上哺乳動物轉錄因子結合序列的保守序列，從而大大降低了實驗的本底，顯著提高了實驗的相對信號強度。優化
隨著發光(luminescence)技術在多種生物實驗中的廣泛應用，生物發光(bioluminescence)技術越來越成為首選的生物檢測手段。在這篇文章中，我們將詳細討論生物發光技術在生物檢測中的應用，以及它與其它發光檢測手段相比所顯示出的優點。 1 生物發光的特點 根據產生光子的
References1. Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J, Jemal A. Global cancer statistics, 2012. CA: a cancer journal for clinician
實驗概要The procedure presented below describes a method for preparing rat liver.主要試劑1. Aluminum Foil2. &nb
Leave tubes on ice and repeat Steps 13–15 for the next 6–12 plates of cells.When all cells are collected, centrifuge all tubes at 250g for 5 min
SDS-PAGE膠樣品排列： MarkerUII 37CUI’I’ Marker：低分子量蛋白marker，上樣10ul
人胚腎 293 (HEK293) 細胞在重組蛋白表達中是最常見的宿主細胞。 這類細胞能夠表達大量的膜蛋白，如 G 蛋白偶聯受體 (GPCR) ，是無法在最常見的生物制藥生產宿主，如：中國倉鼠卵巢 (CHO) 細胞中作表達。 HEK293 雖然是蛋白表達的極好宿主，然而 H
Lysate preparation and western blottingProtein lysates were created by harvesting the cells from con?uent T-?asks or from suspension cultures at h
Preparation of Glutathione-S-Transferase (GST) Fusion ProteinsMargret B. Einarson and Elena N. Pugacheva Foxx Chase Cancer Center, Philadelphia,
實驗概要Preparation of lysis buffers, protease and phosphatase inhibitors, lysate from cell culture, lysate from tissues, protein concentratio
AbstractA general method for the assay of deubiquitinating enzymes was described in detail using 125I-labeled ubiquitin-fused αNH-MHISPPEPESEEEEE
1) After ECL development, wash membrane once for 10min with PBST.2) Incubate the membrane in stripping buffer (see below) in a heat-sealed plastic bag
實驗概要 The immunoprecipitation (IP) of cross-linked chromatin with antibodies specific for certain histone modifications (chromatin &nb
NUCLEAR EXTRACTION OF TISSUE CELLSThis procedure was developed for HNEK cells grown in KGM, from Clonetics. All steps are performed on ice, with
Preparation of cell lysate:1. For cells in suspension, grow in DMEM with 10% FCS and then the day before you do the experiment spin the cells down (1
實驗概要This protocol describes how chromatin is prepared from Arabidopsis, which can subsequently be used for chromatin immunoprecipitation (ChIP). T
Take the appropriate set of Fmoc-amino acid stock aliquots for cycle 1 from the freezer, bring to room temperature, and activate by adding DIC (4 μl p
實驗概要Primary tissues are valuable tools for the study of intracellular and extracellular markers which characterize disease states. We have
實驗概要The procedure presented below describes a method for extracting nuclear from several cell lines of human origin.主要試劑Hypotonic Buffer Solution20 mM