MITOMYCINCTREATMENTOFPMEFs
Cultures to be treated should be sub confluent ie actively growing.1. Add 1/20 volume Mitomycin C (200 ug/ml 10 ug/ml), to culture and incubate at 37 for 2hrs.2. After approx. 2 hrs remove medium and collect into a waste bottle. Cytotoxic waste needs to be kept separate for incineration.3. Wash monolayer with 3 x 10 ml PBS (80 cm2 flask), 4 x 10 ml (175 cm2 flask), collect PBS into waste bottle.4. Add TRYPSIN/EDTA (......閱讀全文
MITOMYCIN－C－TREATMENT－OF－PMEFs
Cultures to be treated should be sub confluent ie actively growing.1. Add 1/20 volume Mitomycin C (200 ug/ml 10 ug/ml), to culture and incubate at 37
胚胎干細胞培養技術大全
MEDIA AND SOLUTIONS REQUIRED FOR ROUTINE ES CELL CULTURE Routine Culturing of ES Cells ISOLATION OF PRIMARY MOUSE EMBRYO FIBROBLASTS MITOM
胚胎干細胞培養
Media and Solution required for ES Cell Culture?(Bowtell Lab)???Routine Culturing of ES Cells?(Bowtell Lab)??Routine Splitting and freezing of cells?(
CIP－Treatment
set up the following reaction:CIP RxnH2O7.8 ml10x cip rxn buffer2.0 mlDNA(e.g; 3 kb vector; 0.2 mg/ml; 2 mg total)10.0 ml(1 u/ml) CIP0.2 mltotal20.0 m
Routine－Culturing－of－ES－Cells
Cell are normally passaged every 2-3 days, this is important to avoid differentiation.Signs of differentiation are:-i) colonies are surrounded by flat
RNAse－A－Treatment－of－Mouse－Cells
IntroductionRNAse A treatment of permeabilized cells followed by immunostaining is a method which allows to show if the localization of a protein into
Formaldehyde－Treatment－of－Tissue－Culture－Hoods
You will need:- 12g Potassium Permanganate? 6g Crushed paraformaldehyde 1) Set the hood so that it can vent outside. 2) Mix the two chemicals abov
DNAse－posttreatment－for－nuclear－antigens
Rationale:? The use of DNAse to improve nuclear antigen staining has been published long before the AR era?1, 2. DNAse treatment is currently sugge
RNase－and－DEPC－Treatment:－Fact－or－Laboratory－Myth
Researchers are usually trained in RNA isolation and analysis methods by one another or by technical manuals. Experimental procedures are often not qu
Effect－of－UV－Treatment－on－Early－Development－of－Sea－Urchin－Embryos
Objective:To test the effects of UV radiation at 254nm on Sea Urchin embryo development after radiation at the 2-cell stage.Background:Sea Urchins exh
[3H]－Choline－Labeling－and－TNF－Treatment－of－HL60－Cells
1) Grow cells to a density of 5-8 X 105 cells/ml in RPMI 1640 containing serum.2) Pellet cells and wash 1 time with room temperature PBS.3) Resuspend
Culture－of－Human－Embryonic－Stem－Cells－(hESC)
All cell lines are initially grown according to the supplier's protocols but we are adapting them to one simple protocol outlined below: 6-well pl
Role－of－BRCA1,－BRCA2－and－ATR－in－Cancer－Susceptibility
BRCA1 and BRCA2 were identified genetically as breast cancer susceptibility genes when a single copy of the gene is mutated and are involved in the ce
鏈霉菌屬的重要作用
鏈霉菌的次級代謝產物種類豐富，最重要的就是產生抗生素。現發現由鏈霉菌產生的抗生素有1000多種，已經應用于臨床的近百種，如鏈霉素(streptomycin)、卡那霉素(kanamycin)、絲裂霉素(mitomycin)，土霉素(oxytetmcycline)等。有的鏈霉菌能產生多種抗生素，還有
stem－cell－culture－protocol
實驗概要stem cell culture protocol主要試劑cell culture supplies and reagentssEnvironment: cell culture requires a sterile environment, so it needs a separat
絲裂霉素的基本信息介紹
絲裂霉素（Mitomycin）為從放線菌的培養液中分離出的抗腫瘤藥物，對多種實體瘤有效，為常用的周期非特異性藥物之一。從結構上看具有苯醌、烏拉坦及乙烯亞胺基三種有效基團。在細胞內通過還原酶活化后，起作用，可使DNA解聚，同時拮抗DNA的復制。高濃度時對RNA和蛋白質的合成亦有抑制作用。主要作用于
Medical－Aesthetics:－Bespoke－Technology－－Trends－to－Embrace－Natural－looks
The hype bolstered by social media influencers and growing awareness about youthful appearance has surged the demand for medical aesthetic procedures
如何分析real－time－PCR的數據結果
學習做Realtime PCR, 實驗結果是出來了，得到的內參、目的基因的Ct 值在15-25 之間，熔融曲線基本上也是單峰，但不太會處理這個結果，對著數據發愁。。。不同的處理方法得到不同的結果。。。做的是相對定量，SYBR Green, 選用2^（-△ △ Ct）法內參基因：對照組1、對照組2
如何分析real－time－PCR的數據結果
學習做Realtime PCR, 實驗結果是出來了,得到的內參、目的基因的Ct 值在15-25 之間,熔融曲線基本上也是單峰,但不太會處理這個結果,對著數據發愁.不同的處理方法得到不同的結果.做的是相對定量,SYBR Green, 選用2^（-△ △ Ct）法內參基因：對照組1、對照組2、對照組3實
Cell－Cycles－－Introduction
The onion root tip and the whitefish blastula remain as the standard introduction to the study of mitosis. The onion has easily observable chromosomes
Experimental－Protocol－for－cDNA－Library－Construction
Experimental Protocol for cDNA Library ConstructionIdentify appropriate celltype over-expressing corresponding gene.Find out if transcription can be s
Preparation－of－Low－Density,－CollagenaseDigested－Splenocytes
1. Dilute 2 ml of 4000 U/ml Collagenase D as follows: 1 ml into 9 ml HBSS/Ca++/Mg++ (=400U/ml) and 1 ml into 39 ml HBSS/Ca++/Mg++ (=100U/ml). Put on i
The－Developmental－Effects－of－Nicotine－on－Chick－Embryos
More than 22 million American women smoke and approximately 25% of these women continue to smoke during pregnancy (Lambers and Clark 1996). Cigarette
血清孕酮在異位妊娠保守治療中的預測價值
【摘要】? 目的：探討治療前血清孕酮水平在異位妊娠保守治療轉歸的預測價值。方法： 選擇異位妊娠病例75例，其中63例藥物治療，采用統一治療方案：甲氨喋呤（MTX） 聯合米非司酮（Ru486）。所有病例治療前及治療第4天、7天、10天、14天行孕酮及β-HCG檢查。分析治療前孕酮及β-HCG相關性及治
簡單AS污水處理中二沉池停留時間一般是多少
經驗參數是：1.5-2.5t/h污水處理 (sewage treatment,wastewater treatment):為使污水達到排水某一水體或再次使用的水質要求對其進行凈化的過程。污水處理被廣泛應用于建筑、農業，交通、能源、石化、環保、城市景觀、醫療、餐飲等各個領域，也越來越多地走進尋常百姓的
Effect－of－ethanol－on－development－of－Danio－reriro
ObjectiveThe objective of the experiment is to determine the effects of ethanol exposure on the embryonic development of zebrafish through observation
利用自動細胞成像系統分析細胞凋亡
簡介細胞凋亡是一種發生在多細胞生物體內的程序性細胞死亡過程！生化反應導致細胞形態和特征變化及細胞死亡。形態變化包括細胞收縮、核分裂、染色質凝縮、染色 DNA 分裂及 mRNA 衰減。細胞凋亡是一種高度調控的過程，通過內在途經對各種壓力源做出響應，包括饑餓、感染、缺氧和氧化應激反應等。線粒體損
2014中國（南京）國際生物醫藥產業博覽會部分海外企業預覽
Regen Lab Application of Autologous Platelet Rich Plasma has been evaluated in various medical disciplines including orthopaedics, sports medic
翼狀胬肉的病因與治療研究現狀（二）
2.2.4其它手術方法2.2.4.1Merest鞏膜技術：國外學者[19]采用此技術治療了800例原發性胬肉。簡要手術步驟如下：局部浸潤麻醉后切除胬肉頭體部，盡可能多的保留球結膜。仔細清理并燒灼角膜緣區，切除病變區直肌止端與角膜之間的Tenon氏囊。沿角膜緣向上向下游離球結膜，9-0縫線間斷縫合球結
Catabolic－pathway－for－asparagine－and－asparate
Aspartate and asparagine are catabolized into oxaloacetate and the Kreb's cycle in a fairly direct pathway for amino acids with four carbons. The