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MaterialsPCR SuperMix High FidelityVF2 primer (5''-TGCCACCTGACGTCTAAGAA-3'')VR primer (5''-ATTACCGCCTTTGAGTGAGC-3'')Deionized, sterile H2Ostrip of PCR tubes2-log DNA ladder0.8% E-Gel? from Invitrogen Corporation in Carlsbad, CAEquipmentDNA Engine OPTICON\texttrademark from MJ Research, Inc. (now Bio-Rad Laboratories, Inc., Hercules, CA)E-Gel? PowerBase? v.4 from Invitrogen Corporation ......閱讀全文
Figure 5: Regulation of CDH5 by TFZFs in several human cancer cell lines.Blue, cells infected with a pMX construct containing the DNA bindin
Cloning PCR products using TA vectorsby Paul N. Hengen, Ph.D. *Methods and reagents is a unique monthly column that highlights current discussion
Metabolic biotinylation of mammalian cell receptors for imagingBakhos A. Tannous , btannous@hms.harvard.edu, Massachusetts General Hospital and Harvar
OverviewThis is a method to assemble two BioBricks using the Clontech In-Fusion PCR Cloning Kit and maintains BioBrick standard formats. There are cur
Acknowledgements The organizer of the workshop acknowledges Dr. Murray Milford, Professor and Interim Head, and Dr. Mark Hussey, Professor and In
In the polymerase chain reaction (PCR), a thermostable DNA polymerase amplifies DNA that is flanked by known sequences. The known sequences correspond
Using siRNA for gene silencing is a rapidly evolving tool in molecular biology. There are several methods for preparing siRNA, such as chemical synthe
Hematopoietic Stem Cell Targeting with Surface-Engineered Lentiviral VectorsEls Verhoeyen and Fran?ois-Lo?c CossetAdapted from Gene Tra
DNA抽提（主要內容如下）· Working with DNA· DNA Extraction from Bacteria and Other Organisms· DNA Extraction f
· Standard PCR Protocol (Molecular Biology Techniques Manual)The followings are described in
Introduction The most important aspect of our cloning vectors is that t
General AdvicePCR allows the production of more than 10 million copies of a target DNA sequence from only a few molecules. The sensitivity of this tec
Site-directed Mutagenesis using PCRMichael P. Weiner, Tim Gackstetter, Gina L. Costa, John C. Bauer, and Keith A. KretzFrom: Molecular Biology: C
6. Simultaneous digestion of the pUC vector with both enzymes in the presence of 3 units of Shrimp Alkaline Phosphatase (
3．討論 隨著近年基因芯片技術的發展，研究者逐漸認識到基于核酸雜交原理的傳統基因芯片缺陷與應用的局限性。隨著PCR技術的進展，特別是熒光定量PCR技術的出現PCR技術已成為生物醫學領域中應用最廣泛的技術。如果一種基因芯片能直接進行PCR反應,而且能夠同時擴增大批可能發生變異的基因顯
Human zinc fingers as building blocks in the construction of artificial transcription factorsKwang-Hee Bae1, 4, Young Do Kwon1, 2, 4, Hyun-Chul Shin1,
3. Methods3.1 RNA Probe Preparation1. Different strategies can be used to prepare template DNA for synthesizing antisense RNA p
Preparation of nucleic acid probesIn standard nucleic acid hybridization assays the probe is labeled in some way. Nucleic acid probes may be made as s
C. Random fragment end-repair, size selection, and phosphorylationSince both sonicated and nebulized DNA fragments usually contain single-stranded end
如今,單克隆抗體藥物以其獨特的作用機制及高效性,在腫瘤和自身免疫疾病的治療中發揮了不可估量的作用,成為全球的研發熱點，目前已有2400個單克隆抗體藥物處于研發及商業化階段。 1975年雜交瘤技術問世[1]。1986年鼠源單克隆抗體藥物Muromonab的上市拉開了單克隆抗體發展的序幕。
Scanning the human genome with combinatorial transcription factor librariesPublished online: 18 February 2003, doi:10.1038/nbt794March 2003 Volume 21
Disruption by Fusion PCRDavid Amberg and Ellen Beasley1) In separate PCR reactions, amplify the 5' and 3' ends of the gene of inter
Selection of siRNA duplexes from the target mRNA sequenceUsing Drosophila melanogaster lysates (Tuschl et al. 1999), we have systematically analyzed t
The siRNA user guide (revised May 6, 2004) Selection of siRNA duplexes from the target mRNA sequenceUsing Drosophila melanogaster lysa
A. Storage .The following properties of reagents and conditions are important considerations in processing and storing DNA and RNA. Heavy metals promo
We have also been able to detect expression of this receptor in all studied tissues, which is consistent with the pleiotropic nature of growth hormone
細胞系 Cell linesThe following CD19-expressing immortalized cell lines were used: Raji (Burkitt’s lymphoma cell line, ATCC-CCL86),Daudi (B lymphobla
3）微雕刻和ISAAC方法分選B細胞微雕刻技術原理[7]是基于軟光刻微陣列芯片識別、克隆抗原特異性B細胞的方法。通過刺激多克隆B細胞，并將其逐個分布到芯片孔內進行培養產生抗體，然后將改芯片孔內抗體轉印至相應的蛋白芯片，通過與目標抗原反應后，再與熒光抗體反應，最后根據熒光抗體染色結果，通過顯微操作將分
siRNA DatabaseSearchable database of Silencer ? Validated and Pre-designed siRNAs to >34,000 human, mouse, and rat targets. All siRNAs in the datab
BCEIA2019 International Summit on Analytical Instrumentation and In Vitro Diagnosis--Registration in ProgressIn recent years，In Vitro Diagnosis has be