LongPCR
Two long PCR steps:First round of the nested PCR step of the end point dilution procedure to quantitate the cDNA.First round of the nested PCR step of the limiting dilution step to amplify single templates. The conditions are exactly the same both times. Reaction CompositionH2O35.5 μL10x Buffer + 15 mM MgCl25.0 μLfinal [Mg2+] = 1.5 mMdNTP6.0 μLfinal [dNTP] = 300 μM eachDS3 (4895-4924)1.0 μLfinal [primer] =......閱讀全文
Long PCR
Two long PCR steps:First round of the nested PCR step of the end point dilution procedure to quantitate the cDNA.First round of the nested PCR step of
Long PCR
Two long?PCR?steps:First round of the nested PCR step of the end point dilution procedure to quantitate the cDNA.First round of the nested PCR step of
Long PCR Reagents and Guidelines
George Church Lab, Harvard Medical SchoolPCR_protocol.html">http://twod.med.harvard.edu/labgc/estep/longPCR_protocol.htmlEfficient Long PCR results fr
Long-PCR Reagents and Guidelines
Long-PCR Reagents and Guidelinesfrom George Church as Modified from Cheng et al. (1)General Guidelines for Long-PCR Conditions and Enzyme Mixtures====
Long Term Storage of Bacterial Strains
Purpose:Bacterial strains may be stored indefinitely at low temperatures (- 20 degrees C and -80 degrees C) in 15 to 40 glycerol. It is lab policy to
Long Term Storage of Transformed E.coli
Long Term Storage of Transformed E.coliTransfer 10 ml of 250 ml overnight culture in sterile flip-cap 15 ml Tube.Add sterile Glycerol to 15 % final co
Method: Preparation of Lymphoblastoid Cell Lines for Long Term Storage
Method: Preparation of Lymphoblastoid Cell Lines for Long Term Storage May 30, 1990 Rosalie Veile Purpose: To store cell lines in a form
利用long-read生成個人轉錄組
斯坦福大學醫學院的遺傳學教授Michael Snyder及其同事利用Pacific Biosciences系統，對三個家庭成員的類淋巴母細胞轉錄組進行了測序，并將獲得的reads與Illumina平臺上獲得的較短reads進行比較。通過這些轉錄組，他們開發出一名家庭成員的等位基因特異的全長轉錄組
Isolation and long-term cultivation of Human Corneal Endothelial Cells
Coating of the Culture Dish SurfacesCulture dishes were incubated with a film of a 1:1 mixture of laminin (10 μg/ml and chondroitin sulfate (10 μg
LONG廣隆蓄電池十大保養方法
LONG廣隆蓄電池是UPS系統中的一個重要組成部分，它的優劣直接關系到整個UPS系統的可靠程度。不管UPS設計的多么先進，功能多么齊備，一旦LONG廣隆蓄電池失效，再好的UPS也無法提供不間斷供電。千萬不要因貪圖便宜而選用劣質鉛酸蓄電池，這樣會影響整個UPS系統的可靠性，并將因此造成更大的損失。