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The DNA Facility houses the “real-time” or kinetic PCR instrument, the Applied Biosystems Model 7700 sequence detection system (the TaqMan instrument). The polymerase chain reaction (PCR) has revolutionized the detection of DNA and RNA. As little as a single copy of a particular sequence can be specifically amplified and detected. Theoretically, there is a quantitative relationship between amount of starting target s......閱讀全文
Lot-No.Ref. FR342 Expiry time: 1 year100 Tests (Ready to use PCR kit) -Only for in vitro use--Only for research use – To be used by a
Lot-No.Ref. FR340 Expiry time: 1 year100 Tests (Ready to use PCR kit) Zika Virus (Real time) DOUBLE CHECK &n
2013年5月16日-19日，由中國化學會主辦、廈門大學承辦、復旦大學、浙江大學協辦的為期四天的第八屆全國微全分析系統學術會議、第三屆全國微納尺度生物分離分析學術會議暨第五屆國際微化學與微系統學術會議在美麗的海濱城市廈門隆重召開。以下是微全分析專場報告。上海交通大學
MicroRNA Expression Profiling by Bead Array Technology in Human Tumor Cell Lines Treated with Interferon-Alpha-2aMicroRNAs are positive and negative r
Bead-array-based microRNA detection technology, including the bio-statistic analysis, is currently not well established or widely used and we have app
聚合酶鏈式反應(Polymerase Chain Reaction，PCR)可對特定核苷酸片斷進行指數級的擴增。在擴增反應結束之后，我們可以通過凝膠電泳的方法對擴增產物進行定性的分析，也可以通過放射性核素摻入標記后的光密度掃描來進行定量的分析。無論定性還是定量分析，分析的都是PCR終產物。但是在許多
慢性腎病病人一般會出現很多的并發癥，其中大多數腎損傷晚期病人都患有嚴重的高血壓。鹽是慢性腎病和高血壓發病的主要致病因素之一，但其中的病理機制仍然不清楚。本文利用蛋白質組和磷酸化蛋白質組聯合分析的技術，對慢性腎病并發高血壓的病理機制進行了全面系統性的分析。 Proteomic and p
慢性腎病病人一般會出現很多的并發癥，其中大多數腎損傷晚期病人都患有嚴重的高血壓。鹽是慢性腎病和高血壓發病的主要致病因素之一，但其中的病理機制仍然不清楚。本文利用蛋白質組和磷酸化蛋白質組聯合分析的技術，對慢性腎病并發高血壓的病理機制進行了全面系統性的分析。 Proteomic and p
INTRODUCTION After chromatin immunoprecipitation (ChIP), different PCR-based approaches can be used to determine how much DNA is p
一滴殘留在裙子上的精液使得美國總統Bill Clinton不得不坦承他與白宮實習生有不正當的關系。因為他知道現在的生物科技就連一個精子也能被用來做為證據。這種將極微量的生物標本化為可供鑒定的現代技術正是PCR(Polymerase chain reaction)--聚合
INTRODUCTIONAfter chromatin immunoprecipitation (ChIP), different PCR-based approaches can be used to determine how much DNA is precipitated at a locu
ContentsFactors Affecting the PCR Nested Primer PCRPrimer LengthDegenerate PrimersElongation Temperature and TimeReaction BufferCycle Numbe
PCRPolymerase Chain Reaction1) Add the following to a microfuge tube:10 ul reaction buffer1 ul 15 uM forward primer1 ul 15 uM reverse primer1 ul templ
Why PrimerBank?PrimerBank is a public resource for PCR primers. These primers are designed for gene expression detection or quantification (real-time
Protocols for LCM preparation and analysis I. Preparation, LCM and RNA/DNA extraction of Frozen Tissue SectionsA. EmbeddingB. CuttingC.
For ionisation to take place at all, chemical reaction between the sample and the&nbs
What is degenerate PCR? Degenerate PCR is in most respect identical to ordinary PCR, but with one major difference. Instead of using
Methylated CpG island Amplification Protocol written by Minoru Toyota2. Materials2.1. MCARestriction enzymes SmaI, XmaIT4 DNA ligaseTaq DNA polym
Non-denaturing PAA gelsTo separate PCR products differing in only a few bp in length (for example, microsatellite markers), 6-10% PAA gels need to be
Metabolic biotinylation of mammalian cell receptors for imagingBakhos A. Tannous , btannous@hms.harvard.edu, Massachusetts General Hospital and Harvar
3. Methods3.1 RNA Probe Preparation1. Different strategies can be used to prepare template DNA for synthesizing antisense RNA p
INTRODUCTIONAfter chromatin immunoprecipitation (ChIP), different PCR-based approaches can be used to determine how much DNA is precipitated at a locu
Degenerate PCR is in most respects identical to ordinary PCR, but with one major difference. Instead of using specific PCR primers with a given sequen
3’ Adaptor Ligation and PurificationHeat shock the RNA by putting at 90°C for 30 seconds. Snap cool on ice.Set up the 3’Adaptor ligation reaction in a
實驗概要 We routinely produce dsRNA by in vitro transcription of a PCR generated DNA template containing the T7 promoter se
General AdvicePCR allows the production of more than 10 million copies of a target DNA sequence from only a few molecules. The sensitivity of this tec
實驗概要 Extension of overlapping gene segments by PCR is a simple, versatile technique for site-directed mutagenesis and g
Influence of annealing temperature and number of loci amplifiedLike any other PCR, multiplex reactions should be done at a stringent enough temperatur
Troubleshooting discussion is based on the PCR protocol as described in the table below. All reactions are run for 30 cycles. COMPONENTVOLUMEFINA
General AdvicePCR allows the production of more than 10 million copies of a target DNA sequence from only a few molecules. The sensitivity of this tec