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Description RNA extraction using TRI REAGENT. This method gives ample amout of RNA.Procedure It is 3 days procedure.Day 1:1. Harvest the cells and centrifuge at 2000 rpm for 5-10 minutes and pour off the media.2. Wash the cell pellet with PBS and add 10 ml TriReagent solution.3. Incubate at Room Temperature for 5 min and during incubation pass the cell suspension through Pipette.4. Collect the solutions in ......閱讀全文
Protocols for LCM preparation and analysis I. Preparation, LCM and RNA/DNA extraction of Frozen Tissue SectionsA. EmbeddingB. CuttingC.
Global expression profiling of RNA isolated from laser microdissected cells allows one to profile a specific set of cells allowing for enhanced se
Andrea J. Gossett and Jason D. Lieb1Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA1Correspon
結論· 我們在C1TM單細胞自動制備系統開發了一種簡潔的實驗方案，能以最少的手工操作，在不到24小時內，平行處理高達96個單細胞，對其miRNA表達譜進行分析。· C1 miRNA STA實驗方案使用了Life Technologies為miRNA優化過的試劑。特別的，Meg
Introduction MicroRNA (miRNAs) are short (18–24 nucleotides), non-coding RNAs that regulate gene expression by both disrupting mess