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General Preparation1. Prepare buffers and have them cold. Make 50X stock of Buffer G and dilute as needed from frozen aliquots of 50X Buffer G.1X Extraction Buffer without detergents: 1 M Tris-Cl, pH 7.0, 0.6 M KCl, 0.5 mM ATP, 1 mM DTT, 0.5 mM MgCl2, 0.2 mM PMSF (add just before use).Buffer D: 10 mM Tris-Cl, pH 8.0, 0.2 mM CaCl2, 0.5 mM ATP, 0.1 mM DTT, 100 mM KCl, 0.1 mM PMSF.Buffer G: 1 mM Tris-Cl, pH 8.0, 0.......閱讀全文
Fixation and Immunofluorescence of the Cytoskeleton (Mitchison Lab) Recycling Tubulin (Mitchison Lab) Labeling Tubulin
實驗概要Invitrogen offers several fluorescent and biotinylated phalloidin and phallacidin derivatives for labeling F-actin. These phallotoxins
實驗概要Invitrogen offers several fluorescent and biotinylated phalloidin and phallacidin derivatives for labeling F-actin. These phallotoxins
The intact mature arterial media is composed of at least four phenotypically unique cell subpopulations that reside in distinct medial layers.&nbs
THESE INSTRUCTIONS APPLY TO ORDERS CONTAINING THE FOLLOWING CELL PRODUCTSCryopreserved Cells (Single donor)CC-2511NHDF -Ad3 500,000 cells/cryovia
肌球蛋白myosin與微絲actin形成具有收縮性的肌動肌球蛋白束，在肌肉細胞中以肌小節形式存在，在非肌肉細胞中以微絲應力纖維形式存在，是細胞定向運動的動力結構，特別對癌細胞的轉移、免疫細胞的靶向運動等生命過程至關重要，但其組裝的分子機制和動態過程研究較少。Myosin-18B基因突變造成新生兒
Isolation and culture of human pulmonary artery smooth muscle cells (PASMCs)1. The human pulmonary arteries were opened to expose the endothelial
1. Ventricles were removed under sterile conditions. 2. Ventricles were placed in cold sterile primary cell culture medium, minced into
CMVE were isolated from Wistar rat hearts by digestion of primary cell isolation kit.1. Hearts mounted on a Langendorff apparatus were perfused
兔膀胱平滑肌細胞培養可以：（1）分離得到高純度兔膀胱平滑肌細胞；（2）進行細胞學鑒定研究；（3）用于細胞學其他研究。實驗方法原理運用顯微手術器械在肉眼下仔細剔除膀胱黏膜層及漿膜層后，完整分離膀胱平滑肌層。然后以酶分離法獲取兔膀胱平滑肌細胞，體外原代和傳代培養分離細胞。在倒置顯微鏡下觀察細胞形態及生長
酶分離法 實驗方法原理 運用顯微手術器械在肉眼下仔細剔除膀胱黏膜層及漿膜層后，完整分離膀胱平滑肌層
收起 注意事項1. 應嚴格無菌操作。2. 仔細徹底剝除膀胱黏膜、黏膜下及漿膜層，是確保獲的大量高質單個膀胱平滑肌細胞的基礎。3. 膀胱平滑肌組織應盡量減碎以確保充分消化。4. 嚴格控制膠原酶和胰蛋白酶的濃度和消化時間，見消化液混濁、組織塊呈絮狀，或在倒置顯微鏡下見消化液中有
實驗方法原理 運用顯微手術器械在肉眼下仔細剔除膀胱黏膜層及漿膜層后，完整分離膀胱平滑肌層。然后以酶分離法獲取兔膀胱平滑肌細胞，體外原代和傳代培養分離細胞。在倒置顯微鏡下觀察細胞形態及生長狀況，同時進行細胞爬片H-E染色和透射電鏡等形態學觀察分析，并應用免疫熒光染色平滑肌特有的α-肌動蛋白進行細胞學鑒
酶分離法 實驗方法原理 運用顯微手術器械在肉眼下仔細剔除膀胱黏膜層及漿膜層后，完整分離膀胱平滑肌層
DNA抽提（主要內容如下）· Working with DNA· DNA Extraction from Bacteria and Other Organisms· DNA Extraction f
RNA提取（主要內容如下）Tips for Handing RNA Total RNA IsolationmRNA IsolationrRNA IsolationOthersQ & A posted in the Method Forum Basic Proc
實驗概要DNAzol? Reagent (Genomic DNA Isolation Reagent) is a complete and ready-to-use reagent for the isolation of genomic DNA from solid and
Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed CellsActin: Louise CramerTubulin: Arshad DesaiGeneral StrategyWe typically wor
B. Midiprep double-stranded DNA isolationA midi-prep double-stranded DNA isolation has been developed to generate a sufficient amount of template DNA
Intestinal intraepithelial lymphocytes Intestinal intraepithelial lymphocytes (IELs) are mostly T cells, which are continuously
General StrategyWe typically work with tissue culture, primary mammalian cells, and cell extracts, but the protocols can be adapted to other systems,
DNA電泳（主要內容如下） Preparation of Agarose Gel and Electrophoresis Extraction of DNA From Agarose Gel Extraction of DNA fro
DNA Extraction· DNA Extraction from Bacteria (Julie B. Wolf,UMBC)Phenol/chloroform method·&n
實驗概要Human embryonic stem cells can be differentiated into neural-, mesenchymal and hematopoetic stem cells. This product is intended for t
Recent Advancements in mmWave Isolator TechnologyMarch 13, 2019 David W. Porterfield, Jr., Micro Harmonics Corp., Fincastle, Va. No Comments
實驗概要Interest in RNA-protein interactions is booming as we begin to appreciate the role of RNA, not just in well-established processes such as tran
DNA Isolation by a Cleared Lysate Method Followed by Double Acetate Precipitation Version 3b - updated September 26, 1999The Most Recent Roe Lab Imple
Immunofluorescence Microscopy of tissue culture cellsThese methods are written for direct staining of filamentous actin with bodipy FL-phallicidin and
實驗概要Plant DNAzol? is an extra-strength-DNAzol? reagent (patent pending) specifically formulated for the isolation of genomic DNA from plants. The Plan
DNA PreparationGene TransferEmbryo TransferTransgenic IdentificatioinOthersTransgenic Outline (University of Michigan Transgenic Animal Model Cor