PulseFieldElectrophoresis
Manipulating and analyzing DNA are fundamentals in the field of molecular biology. Indeed, separating complex mixtures of DNA into different sized fragments by electrophoresis was a well-established technique by the early 1970's.Typically, DNA was isolated intact and then treated with restriction enzymes to generate pieces small enough to resolve by electrophoresis in agarose or acrylamide. Although this procedur......閱讀全文
Pulse Field Electrophoresis
Manipulating and analyzing DNA are fundamentals in the field of molecular biology. Indeed, separating complex mixtures of DNA into different sized fra
Dark Field Viewing
Dark Field ViewingPrincipleTo view a specimen in dark field, an opaque disc is placed underneath the condenser lens, so that only light that is scatte
脈沖[交變]電場凝膠電泳
中文名稱脈沖[交變]電場凝膠電泳英文名稱pulse [alternative] field gel electrophoresis定 義利用脈沖電場的作用在凝膠介質中分離大分子DNA的一種電泳方法。由于超過一定大小(大于40 kb)的線狀DNA分子在瓊脂糖凝膠中電泳的速度幾乎相同,無法在恒場強凝膠
An Integrative Procedure for Apoptosis Identification and Measurement-2
TroubleshootingCritical Steps(1) Don’t trypsinize cells for too long when collecting them.(2) Rotation speed should be no more than 1500 rpm during ce
Construction of BAC Libraries:Construction of a BAC library
Once high molecular weight (HMW) DNA has been prepared it must somehow be fragmented and DNA in the desired size range isolated. In general, as the de
PURELAB Pulse的EDI技術
連續電去離子技術連續電去離子技術(EDI,Electrodeionization)是一種融合了電滲析技術,離子交換(IX)技術和陽、陰離子選擇透過膜的先進綠色環保的水處理技術。經過反滲透膜處理后的純水進入EDI模塊。在電場的作用下,水中離子在樹脂中定向遷移,通過陽、陰離子選擇透過膜。這些離子集中到濃
Chromatin Electrophoresis
Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College Exercise 10.4 - Chromatin Electrophoresis LEVEL II Materials ? 14 M Ure
Capillary Electrophoresis
Capillary electrophoresis is a very sensitive analytical technique. Sample components are separated within a fused silica capillary using one of seve
RNA Electrophoresis
Electrophoresis through agarose or polyacrylamide gels is the standard way to separate, identify and purify nucleic acid fragments. The location
Protein Electrophoresis
DefinitionAmino acids, nucleotides, polypeptides, and other compounds in a colloidal state can be separated by the application of external voltages wh