COLONYHYBRIDIZATION
COLONY HYBRIDIZATION1) CUT 4 PIECES OF 3MM WHATMAN PAPER AND PLACE EACH ONE IN A SEPARATE CONTAINER(A CAFETERIA TRAY WILL PROBABLY WORK PERFECTLY).2) SATURATE EACH OF THE 4 PIECES WITH ONE OF THE FOLLOWING SOLUTIONS:A) 10% SDSB) Denaturing Solution (.5N NaOH, 1.5M NaCl)C) Neutralizing solution (1.5M NaCl,.5M Tris pH 7.4)D) 2X SSC** DO NOT ALLOW THE FILTERS TO BECOME TOO WET BECAUSE THE COLONIES WILL SWELL AND DIFFUSE......閱讀全文
COLONY HYBRIDIZATION
COLONY HYBRIDIZATION 1) CUT 4 PIECES OF 3MM WHATMAN PAPER AND PLACE EACH ONE IN A SEPARATE CONTAINER(A CAFETERIA TRAY WILL PROBABLY WORK PERFECTLY).
Colony Hybridization
ProcedurePrepare serial 10-fold dilutions of transformed bacteria in LB and spread 100 μL onto LB/Amp plates, as described in the Electroporation prot
Colony PCR
Colony?PCR David Amberg This procedure will work for both yeast and E. coli: Take a small colony and suspend it in 5ul of H2O in a PCR tube. Heat
Colony PCR
Colony PCR is useful in determining whether or not a specific colony on a plate has a sequence you desire. Primers for the specific sequence should b
In situ hybridization
Note:?Although it is possible to perform in situ hybridization on bleached embryos, it appears to reduced the strength of the signal. For best res
Silver: Colony PCR
Zymolyase Solution: Regular stock of zymolyase is 10 mg/ml diluted in water, mix by inverting, not all will go into solution (filter it) store ali
Bacterial Colony PCR
Bacterial Colony?PCRObjective:This protocol allows rapid detection of transformation success when primers are available to allow determination of corr
Colony PCR Protocol
1. Pull out eight glycerol stock plates from the –80oC freezer and set on bench top to thaw. Be sure to remove the foil seal before leaving the plates
Microarray Hybridization Protocol
Introduction:One microarray set consists of 7 nylon membranes with 2.5 x 7.5 cm dimension. 2304 genes were spotted onto nylon membranes (Schleicher an
Blackburn:Yeast Colony PCR
OverviewThis is a quick and easy yeast colony PCR protocol that does not require zymolyase step.Updated Protocol:?Blackburn Lab: Quick and Easy Yeast