Silver:ColonyPCR
Zymolyase Solution:Regular stock of zymolyase is 10 mg/ml diluted in water, mix by inverting, not all will go into solution (filter it) store aliquots at -20°C (minimize freeze-thawing if possible)Make 2.5 mg/ml zymolyase solution in 0.1 M sodium phosphate buffer pH 7.5 immediately before PCR experimentLysis of Yeast:Aliquot 15 μl 2.5 mg/ml zymo solution (in 0.1 M sodium phosphate buffer pH 7.5) into thin-walled PCR ......閱讀全文
Silver:－Colony－PCR
Zymolyase Solution: Regular stock of zymolyase is 10 mg/ml diluted in water, mix by inverting, not all will go into solution (filter it) store ali
Colony－PCR
Colony?PCR David Amberg This procedure will work for both yeast and E. coli: Take a small colony and suspend it in 5ul of H2O in a PCR tube. Heat
Colony－PCR
Colony PCR is useful in determining whether or not a specific colony on a plate has a sequence you desire. Primers for the specific sequence should be
Colony－PCR－Protocol
1. Pull out eight glycerol stock plates from the –80oC freezer and set on bench top to thaw. Be sure to remove the foil seal before leaving the plates
Bacterial－Colony－PCR
Bacterial Colony?PCRObjective:This protocol allows rapid detection of transformation success when primers are available to allow determination of corr
菌落PCR（Colony－PCR）方法
菌落PCR（Colony PCR）可不必提取基因組DNA，不必酶切鑒定，而是直接以菌體熱解后暴露的DNA為模板進行PCR擴增，省時少力。建議使用載體上的通用引物。通常利用此方法進行重組體的篩選或者DNA測序分析。最后的PCR產物大小是載體通用引物之間的插入片斷大小。具體方法：1、PCR混合物的制備T
Blackburn:Yeast－Colony－PCR
OverviewThis is a quick and easy yeast colony PCR protocol that does not require zymolyase step.Updated Protocol:?Blackburn Lab: Quick and Easy Yeast
Endy:Yeast－Colony－PCR
MethodUsing sterile pipette tips, transfer a 1 mm colony into 50 uL of 60 U/ml Zymolyase3 uL of 1 U/mL Zymolyase stock solution47 uL of waterIncubate
菌落PCR（Colony－PCR）具體方法
菌落PCR（Colony PCR）可不必提取基因組DNA，不必酶切鑒定，而是直接以菌體熱解后暴露的DNA為模板進行PCR擴增，省時少力。建議使用載體上的通用引物。通常利用此方法進行重組體的篩選或者DNA測序分析。最后的PCR產物大小是載體通用引物之間的插入片斷大小。具體方法：1、PCR混合物的制
Engineering－BioBrick－vectors－from－BioBrick－parts/Colony－PCR
MaterialsPCR SuperMix High FidelityVF2 primer (5''-TGCCACCTGACGTCTAAGAA-3'')VR primer (5''-ATTACCGCCTTTGAGTGAGC-3'')De
Silver－Enhancement－...
實驗概要The method provides a silver enhancement protocol for immunoassay.主要試劑Prepare the following reagents fresh daily except for the citrate buffer.1.
COLONY－HYBRIDIZATION
COLONY HYBRIDIZATION 1) CUT 4 PIECES OF 3MM WHATMAN PAPER AND PLACE EACH ONE IN A SEPARATE CONTAINER(A CAFETERIA TRAY WILL PROBABLY WORK PERFECTLY).
Colony－Hybridization
ProcedurePrepare serial 10-fold dilutions of transformed bacteria in LB and spread 100 μL onto LB/Amp plates, as described in the Electroporation prot
Silver－Staining－Protocol
1x 40min - overnight?????50% MeOH, 12% Acetic Acid 1x 30min??????????????????????????????????50% MeOH, 12% Acetic Acid, 0.05% 37% Formaldehyde 3x 2
Silver:－Lysate－for－Western
Grow cellsHarvestSpin downWash with PBSSpin down enough cells to collect a 50-100 μL cell pellet in a FastPrep tubePrepare lysis buffer (beforehand) a
Silver－Acetate－Autometallography－(AMG)
In the early eighties, a series of papers were published by Gorm DANSCHER, Aarhus, Denmark, to introduce a reliable and easy-to-handle technique for
Silver:－TimeLapse－Microscopy
Pad Preparation 1. Microwave 2% agarose (mix of low-melt and normal, to taste) in Thorn media (see below). (If you have used different percentages o
SSR－GEL－and－Silver－Staining－Protocol
I.?EQUIPMENT:DNA sequencing unit (35 x 45 cm) & 2000V power supplyClampsLg. plastic trays (4), about 43 x 50 x 8 cm, and one lidTwo rocking platformsH
SOFT－AGAR－ASSAY－FOR－COLONY－FORMATION
Note: All volumes are calculated to cater for four plates per point.Base Agar1. Melt 1% Agar (DNA grade) in microwave, cool to 40 in a waterbath. War
SDI檢測儀XFsilver
SDI檢測儀/污染指數儀/污染指數測定儀 型號：XF-silver 指數（SDI）值，也稱之為FI（Fouling Index）值，是水質指標的重要參數之一。它代表了水中顆粒、膠體和其他能阻塞各種水凈化設備的物體含量。通過測定SDI值，可以選定相應的水凈化技術或設備。 在反滲透水處理
SDI檢測儀XFsilver
SDI檢測儀/污染指數儀/污染指數測定儀 型號：XF-silver 指數（SDI）值，也稱之為FI（Fouling Index）值，是水質指標的重要參數之一。它代表了水中顆粒、膠體和其他能阻塞各種水凈化設備的物體含量。通過測定SDI值，可以選定相應的水凈化技術或設備。 在反滲透水處
銀染(silver－staining)操作規程
實驗原理：在堿性條件下，用甲醛將蛋白帶上的硝酸銀（銀離子）還原成金屬銀，以使銀顆粒沉積在蛋白帶上。染色的程度與蛋白中的一些特殊的基團有關，不含或者很少含半胱氨酸殘基的蛋白質有時候呈負染。銀染的詳細機制還不是非常清楚。 試劑：乙醇、冰醋酸、乙酸鈉、硫代硫酸鈉、硝酸銀、碳酸鈉、甘氨酸或EDTA.Na
In－Vitro－prostate－colony－and－sphereforming－assays
1.?Prostates were dissected, minced into small pieces with a steel blade, and digested with 0.8 mg/ml collagenase in 10 ml of primary cell medium/
低背景的蛋白質銀染(silver－staining)方法
我們做蛋白質電泳的人都知道，銀染色很靈敏，有很強的說服力，但通常膠背景較深，不易掃描。在這里介紹一下低背景的蛋白質銀染方法。Step Reagent Time/minFix 50%EtOH, 12%HAC, 0.1%HCHO, 38%H2O 60Rinse 50% EtOH 5 min, 3 tim
Cell－and－tissue－lysis－hub
This page should point you to the many different general and lab-specific protocols describing tissue and cell lysis and serve as forum for comparison
基因型分析
Randomly Amplified Polymorphic DNA (RAPD)Randomly Amplified Polymorphic DNA (RAPD)?by??(DNA KAFFE)RAPD analysis has been successfully used in mapping
造血干細胞CFU（Colony－Forming－Unit）集落形成檢測
實驗概要造血干細胞CFU（Colony Forming Unit）集落形成檢測主要試劑DPBS，HSCs培養液，MethoCult?培養基，HSCs稀釋液主要設備離心機，超凈臺，體視鏡，倒置顯微鏡，35 mm、100 mm培養皿，注射器，16號鈍針頭，移液器，渦旋儀，5 mL、10 mL移液管，離心
Single－tube－confirmation－PCR－protocol
The following colony PCR protocol has been designed to be performed in individual reaction tubes. We usually test three colonies from each transformat
PCR－protocol
PCR reactionProtocol for 50μl reaction - adjust amounts if necessary, for a 20μl reaction use the same volumes of primer and dNTP-mix, but adjust the
Direct－PCR－from－Whole－Yeast－Cells:－Zymolyase－Method
Direct PCR from Whole Yeast Cells: Zymolyase MethodContributor: Namjin ChungDate: June 18, 19961. An average-size yeast colony (0.5-2mm) or a cell pel