SilverStainingProtocol
1x 40min - overnight 50% MeOH, 12% Acetic Acid1x 30min 50% MeOH, 12% Acetic Acid, 0.05% 37% Formaldehyde3x 20min &nbs......閱讀全文
Silver Staining Protocol
1x 40min - overnight?????50% MeOH, 12% Acetic Acid 1x 30min??????????????????????????????????50% MeOH, 12% Acetic Acid, 0.05% 37% Formaldehyde 3x 2
SSR GEL and Silver Staining Protocol
I.?EQUIPMENT: DNA sequencing unit (35 x 45 cm) & 2000V power supply Clamps Lg. plastic trays (4), about 43 x 50 x 8 cm, and one lid Two rocking
Intracellular Staining Protocol
1.?Fix cells- Add16% formaldehyde directly into culture medium to obtain a final concentration of 1.5% formaldehyde. 2. Incubate in fixative for 10
Actin StainingActin Staining Protocol
實驗概要Invitrogen ?offers several fluorescent and biotinylated phalloidin and phallacidin ?derivatives for labeling F-actin. These phallotoxins, isolated
Direct/Indirect Staining Protocol
Use this Protocol for Directly or Indirectly staining cells for Flow Cytometric Analysis1) Dilute cells to 5x10^6 cells/mL2) Aliquot 100uL of cells pe
Intracellular Cytokine Staining Protocol
實驗概要A ?modification of the basic immunofluorescence staining and flow ?cytometric analysis protocol can be used for the simultaneous analysis ?of surf
銀染(silver staining)操作規程
實驗原理：在堿性條件下，用甲醛將蛋白帶上的硝酸銀（銀離子）還原成金屬銀，以使銀顆粒沉積在蛋白帶上。染色的程度與蛋白中的一些特殊的基團有關，不含或者很少含半胱氨酸殘基的蛋白質有時候呈負染。銀染的詳細機制還不是非常清楚。 試劑：乙醇、冰醋酸、乙酸鈉、硫代硫酸鈉、硝酸銀、碳酸鈉、甘氨酸或EDTA.Na
Methylene Blue DNA staining protocol
Methylene Blue DNA staining protocolProtocol:Load 2-5X the amount of DNA that would give bands of moderate intensity on an ethidium bromide stained ge
Cell Surface Immunofluorescence Staining Protocol
實驗概要A method of identifying ?and enumerating specific cell types in a heterogeneous population of ?cells by enhancing the specific staining of desired
Protocol for intracytoplasmic staining of cytokines for FACS analysis
Description Protocol for intracytoplasmic staining of cytokines for FACS analysis? Procedure 1) Prepare spleen, lymph node or T cell clone cells as