DeterminationandDetectionofReactiveOxygenSpecies(ROS),Lipid...
Reactive oxygen species or intermediates are formed by the incomplete reduction of oxygen. Organisms living in aerobic environment generate various kinds of reactive oxygen species (ROS) molecules, such as superoxide (?O2 ? ), hydrogen peroxide (H2 O2 ), hydroxyl radical (OH? ), singlet oxygen, and lipid hydroperoxides. ROS are highly reactive molecules and are extremely unstable, so detection of ROS relies......閱讀全文
Determination－and－Detection－of－Reactive－Oxygen－Species－(ROS),－Lipid－...
Reactive oxygen species or intermediates are formed by the incomplete reduction of oxygen. Organisms living in aerobic environment generate variou
Bradford－–－Protein－Determination
Bradford – Protein DeterminationIntroductionA rapid and accurate method for the estimation of protein concentration. The technique is simpler, faster
Lowry－–－Protein－Determination
Lowry – Protein Determination(From Protein Protocols on CD-ROM Humana Press, 1998 - Section 1-2 The Lowry Method for Protein Quantitation Jakob H. Wat
Coenzyme－A－Detection
實驗概要The experiment provided ?an ?easy, convenient assay to measure the CoA level in a variety of ?biological samples. In the assay, free CoA is specif
ThiolReactive－Probe－Labeling－Protocol
實驗概要 Invitrogen ?offers several fluorescent and biotinylated phalloidin and phallacidin ?derivatives for labeling F-actin. These phallotoxins, isola
Determination－of－IC50
DescriptionIn vitro whole cell assay by [H3] Hypoxanthine uptake assay?Procedure[H3] Hypoxanthine uptake assay (with respect to red blood cell culture
Detection－and－Measurement－of－Radioactivity
Radioactive Decay Isotopes of a given element have nuclei with the same number of protons but different numbers of neutrons. Some isotopes are st
Detection－of－Glycoproteins－on－Blot
Detection of Glycoproteins on BlotSource:?Contributed by Sharad Purohit, Paller's LabReagentsSodium acetate Buffer (200mM, pH 5.5): Prepare? a 200
Detection－of－Mycoplasma－by－Culture
AimDetection of mycoplasma by culture is the reference method of detection and has a theoretical level of detection of 1 colony-forming unit (cfu). Ho
Detection－and－Measurement－of－Radioactivity
Liquid scintillation countingThe amount of kinetic energy in a beta particle differs from one decay to the next. However, each radioisotope has a typi
Detection－by－TUNEL－labeling
In Situ Cell Death (Apoptosis) Detection by TUNEL labelingby Boehringer Mannheim (Catalog No. 1684809), modified by Josiah N. Wilcox,José C. Rodriguez
COMPARISION－OF－DIFFERENT－PROTEIN－DETERMINATION－METHODS
COMPARISION OF DIFFERENT PROTEIN DETERMINATION METHODSCompanyMethodDetection??RangeApplications?-CompatibilityAssay protocolPrecautions-InterferencesA
FACS－Procedures－for－Apoptosis－Detection
Materials:Hoechst?33258?(Sigma B-2883).stock: 10 mg/ml in dH20 (40)working dilution: 500μg/ml (50μl stock + 950μl PBS).7-Amino-actinomycin (Sigma A-94
UV－Absorbance－(280－nm)－－–－Protein－Determination
UV Absorbance (280 nm)? – Protein Determination?Simple and quick method to accurately quantitate total protein in purified material or approximately q
Quantitative－Determination－of－Peptides－by－Sulfhydryl－(SH)－Groups
Quantitative Determination of Peptides by Sulfhydryl (-SH) GroupsAuthor:?David Van Horn, Greg BulajSource:?Contributed by David Van Horn, Dept. of Che
Bespoke－Metal－Detection－Conveyor－Systems
Many metal detection applications do not fit into the scope of ? standard conveyor systems. For this reason, METTLER TOLEDO SAFELINE are able to
Protein－detection－onto－PVDF－membranes
2-D PAGE and electroblotting onto PVDF membranes have become widely used techniques for the characterization of proteins. Recent improvements have al
Detection－of－Intracellular－Antigens－by－Flow－Cytometry
實驗概要Fix and Perm ?reagents are designed for use with all commercially available flow ?cytometers. Alignment and compensation should be performed accor
DNA－Immunoprecipitation－for－the－Determination－of－DNABinding－Specificity
Andrea J. Gossett?and?Jason D. Lieb1Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA1Corresponding autho
Cell－death－detection－in－Xenopus－embryos－by－ELISA
Sample preparation Wash embryos 1 x in 25% MMR Remove excess buffer Add 10 volumes "incubation buffer", i.e. 50 μl for 5 embryos Lyse the embryos
Detection－of－apoptotic－process－in－situ－using－immunocytochemical
1. INTRODUCTION??Apoptosis was observed from invertebrates to lower and higher verterbrates, and intervenes both in physiological and in pathological
Detection－of－BrdU－Incorporation－in－DNA－Synthesizing－Cells
Detection of BrdU Incorporation in DNA Synthesizing Cells?NOTE:?Bromodeoxyuridine is a known carcinogen. Propidium iodine (PI) is known to be toxic an
PCR－Primers－For－Gene－Expression－Detection－or－Quantification
Why PrimerBank?PrimerBank is a public resource for PCR primers. These primers are designed for gene expression detection or quantification (real-time
The－Determination－of－Proteinprotein－Interactions－by－the－Matingbased－...
Dynamic and reversible protein–protein interactions have a pivotal function in all living cells. For instance, protein–protein interactions are i
Swine－as－a－Principal－R－e－servoir－of－Hepatitis－E－Virus－That－Infects－...1
Swine as a Principal R e servoir of Hepatitis E Virus That Infects Humans in Eastern ChinaYingjie Zheng,1,a Shengxiang Ge ,2,a Jun Zhang, 2 Qingshun G
Detection－Of－Cell－Viability－And/Or－Apoptosis－By－Flow－Cytometry－(FACS)
Viable?cells are cells that when allowed to continue beyond the timepoint of examination will stay alive. Besides live and healthy cells, cells in ear
Detection－of－MicroRNA－Heterogeneity－in－Single－Cells－Using－an－Automated
Introduction ?MicroRNA ?(miRNAs) are short (18–24 nucleotides), non-coding RNAs that regulate ?gene expression by both disrupting messenger RNA (mRNA
Staining－Procedure－for－Flow－Cytometric－Detection－of－Human－Cyclins
Staining Procedure for Flow Cytometric Detection of Human CyclinsThis is a standard protocol used at Pharmingen for Quality Control testing of the ant
In－Situ－Cell－Death－(Apoptosis)－Detection－by－TUNEL－labeling
Protocol for Frozen Sections:Warm 150ml 4% Paraformaldehyde/1x PBS to RT. Fix slides in it, 20 min., RT.1x PBS rinse, 2 times.1x PBS, 30 min., RT. Beg
蛋白質定量分析（Protein－determination）
Bradford 的dye-binding method 是利用Coomassie brilliant blue G-250 （CBG） 可與蛋白質結合而變色的特性來定量 （Bradford, 1976）；若試樣中的蛋白質量較多，則結合到蛋白質而變色的CBG 也多，因而呈色較深。下例是以一組