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BASIC THEORY AND USE OF GC-MSbyDr. Eugenia SobolevaContent1. Introduction.2. GC-MS systems and components.3. Vacuum system3.1. Rotary pump3.2. Diffusion pump3.3. Turbomolecular pump4. Interfacing GC and MS4.1. Packed column interfacing4.......閱讀全文
3.2. Molecular Absorption Attenuation (MAA)As the electromagnetic wave at frequency passes through a transmission medium of distance ,
實驗概要This method is use to extract short RNAs from plant tissue. Some of the variables (e.g. centrifugation speeds×, precipitation
PCR primers should be free of significant complementarily at their 3'-termini as this favours formation of primer-dimer which reduce product yield
9. Spontaneous germination. Some genotypes result in spontaneous ascospore germination (for example, the unpigmented ascospores of per-1 Type I). A si
Fine Mapping of Genomic Targets of Nuclear Proteins in Cultured CellsAchim Breiling and Valerio OrlandoDulbecco Telethon Institute, Institute of Genet
AbstractChIP-Chip stands for Chromatin Immunoprecipitation and chip in the sense of DNA microarray. It is a technique to determine the genom
【摘要】本文選取不論是對于眾多學科的基礎理論方面，還是在人類的生產、生活方面都有重大貢獻的核磁共振研究作為典型案例進行研究，清晰地呈現出了核磁共振研究鮮明的階段性特征，以及由這一典型案例所揭示出的基礎研究與應用研究之間動態變化著的、復雜的互動關系。最后通過分析和總結，得出了這一典型案例對我國的科
【摘要】本文選取不論是對于眾多學科的基礎理論方面，還是在人類的生產、生活方面都有重大貢獻的核磁共振研究作為典型案例進行研究，清晰地呈現出了核磁共振研究鮮明的階段性特征，以及由這一典型案例所揭示出的基礎研究與應用研究之間動態變化著的、復雜的互動關系。最后通過分析和總結，得出了這一典型案例對我國的科
Gastrulation has been celled "the most important time of your life" (Lewis Wolpert); without it you would be flat like a pancake! Vertebrate
A few cell types are thin enough to be viewed directly in a microscope (algae, protozoa, blood, tissue cultures), but most tissues (kidney, liver, bra
Phytohemaglutinin (PHA)Available as kidney bean lectin. It is typically used as a stock solution of 10-20 g/ml in balanced salt solution. For tissue c
實驗概要Basic Methods of Culturing Drosophila實驗步驟Stockkeeping1. Mechanics Most stocks can be successfully cultured by periodic
For ionisation to take place at all, chemical reaction between the sample and the&nbs
Take the appropriate set of Fmoc-amino acid stock aliquots for cycle 1 from the freezer, bring to room temperature, and activate by adding DIC (4 μl p
美國當地時間2月8日，美國工程院公布2017年入選院士名單，共新增84位院士及22位外籍院士。其中有多位著名華人學者，包括黃永剛、沈向洋、張東曉、叢京生、陳向力等人。 美國國家工程院院士是工程界最高榮譽之一，授予那些在工程領域內從事研究、實踐和教育并作出卓越貢獻的人士；以及在新興和發展中的技
Degenerate PCR is in most respects identical to ordinary PCR, but with one major difference. Instead of using specific PCR primers with a given sequen
What is degenerate PCR? Degenerate PCR is in most respect identical to ordinary PCR, but with one major difference. Instead of using
4. Challenges and Solutions for 3D Printed MmWave and THz DevicesThe two dominantly influential factors on the performance of 3D printed mmWave an
實驗概要Capture ELISA (also known as "sandwich" ELISA) is a sensitive assay to quantitate picogram to microgram quantities of substa
2.5 光模數轉換隨著數字信號處理技術的飛速發展，雷達回波的信息提取基本上都在數字域完成。作為連接模擬域回波和數字信號間的橋梁，ADC在雷達接收機中發揮著重要的作用。由于ADC孔徑抖動等原因，大的模擬帶寬和高的有效位數在完全基于電子技術的ADC中難以兼得。因此，電ADC的性能往往成為限制寬帶雷達發展
This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor
實驗概要Live cell studies of cellular DNA content and cell cycle distribution are useful to detect variations of growth patterns due to a vari
Preparation of protein samplesIntracellular virus proteinsThe following method has been developed principally for the analysis of intracellular protei
REFERENCES:R. Ian Freshney, Culture of Animal cells: A manual of basic techniques, Wiley-Liss, 1987.VI. TISSUE CULTURE PROCEDURESEach s
實驗概要Directed differentiation of specific lineages has been a focal point in the field of human embryonic stem cell (hESC) research. Cell r
The single most important advance in the use of centrifugal force to separate biologically important substances. was the coupling of mechanics, optics
The reason for the exponential decay is the resistance that is placed in parallel to the feedback capacity. This solution is necessary so
DNA抽提（主要內容如下）· Working with DNA· DNA Extraction from Bacteria and Other Organisms· DNA Extraction f
實驗概要Live cell studies of cellular DNA content and cell cycle distribution are useful to detect variations of growth patterns due to a vari
Harmonic balance simulation speeds RF mixer designHarmonic balance simulation speeds RF mixer designStephen Maas, Chief Technical Officer, Appli