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Coating of the Culture Dish SurfacesCulture dishes were incubated with a film of a 1:1 mixture of laminin (10 μg/ml and chondroitin sulfate (10 μg/ml) solubilized in selection medium at room temperature for 1-2 hr. The coating solution was aspirated and the dishes washed once with HBS.Subcultures of Human Corneal Endothelial Cells1. Corneas were removed from postmortem human eyes.2. Corneas were excis......閱讀全文
Laser-assisted derivation of human embryonic stem cell lines from IVF embryos after preimplantation genetic diagnosis.T. Turetsky, E. Aizenman, Y. G
Originally described in 1979 and more recently modified by Stamer et al primary trabecular monolayer cell culture has been a cornerstone for inves
DNA抽提（主要內容如下）· Working with DNA· DNA Extraction from Bacteria and Other Organisms· DNA Extraction f
Materials and MethodsShort-term cell growth evaluationLonza? Poietics? human mesenchymal stem cells (hMSC-BM, PT-2501, Lonza) derived from normal ad
Adrenal chromaffin granule (chromaffin vesicle) preparationIntroduction. This prep is adapted from the classic paper of Smith and Winkler (Smith
Human zinc fingers as building blocks in the construction of artificial transcription factorsKwang-Hee Bae1, 4, Young Do Kwon1, 2, 4, Hyun-Chul Shin1,
Thrombin is an extracellular protease that is involved in the clotting of blood and inflammation through its action on platelets and endothelial cells
Stromal stem cells proliferate in vitro and may be differentiated along several lineages. The scarcity of stromal stem cells in tissues and the la
Isolation of AE Cells1. Human placentae were obtained with the approval of the institutional review board, after uncomplicated elective caesa
1. Human microvascular endothelial cells (HMVECs) with primary cell kits were cultured on collagen type I-coated dishes to 80% confluency, th
實驗概要DNAzol? Reagent (Genomic DNA Isolation Reagent) is a complete and ready-to-use reagent for the isolation of genomic DNA from solid and
References1. Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J, Jemal A. Global cancer statistics, 2012. CA: a cancer journal for clinician
THESE INSTRUCTIONS APPLY TO ORDERS CONTAINING THE FOLLOWING CELL PRODUCTSCryopreserved Cells (Single donor)CC-2511NHDF -Ad3 500,000 cells/cryovia
實驗概要Central neurons lose the ability for axonal regrowth during development and typically do not regenerate their axons following axotomy once the
Carbohydrate Assay (Hancock Laboratory) (Accessible only by IE)This protocol is used to determine the relative amounts of LPS CHO present in a gi
Genomic DNA libraries Size of some genomes and chromosomes:Comparative Sequence Sizes(Bases)(yeast chromosome 3)350 ThousandEscherichia coli (bac
結論· 我們在C1TM單細胞自動制備系統開發了一種簡潔的實驗方案，能以最少的手工操作，在不到24小時內，平行處理高達96個單細胞，對其miRNA表達譜進行分析。· C1 miRNA STA實驗方案使用了Life Technologies為miRNA優化過的試劑。特別的，Meg
Table 2: Binding sites and identity of ZFPs used in VEGF activationWe then generated artificial transcription factors by fusing the three-fi
實驗概要The ChargeSwitch? gDNA Purification Kits allow rapid and efficient purification of genomic DNA from small volumes of human blood. Afte
Questions 1: Isolation of hepatocytes from other speciesThe basic two-step perfusion procedure can be used for obtaining hepatocytes from vari
Introduction MicroRNA (miRNAs) are short (18–24 nucleotides), non-coding RNAs that regulate gene expression by both disrupting mess
7. Resuspend the pellet with 3 ml S1 solution (Figure 3). The pellet should be resuspended readily by pipetting up and down. A pellet that cannot be r
Materials and MethodsCell culture conditions and surface transitionCryopreserved hiPSCs (SC102A-1, SBI?, USA) were initially thawed and pre-cultivat
Glomerular endothelial cells (GECs) from the kidney are in close juxtaposition to other cell types, such as mesangial cells, and may respond to as
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In VitroRoxanne Holmes and Juan Carlos Zú?iga-Pfl
3. If your result falls into any quadrant other than the "High Yield-High Viability" quadrant, refer to Appendix D, Improving Cell Yield and
五、使用儀器發表文章AuthorDateTitleJournalCell TypeCellometer / ApplicationsMahato, Ram INovember 2013Synthesis and Characterization of an Anti-Apoptotic Immu
實驗概要A novel impulsive cell pressurization experiment has been developed using a Kolsky bar device to investigate blast-induced traumatic b
Dynamic Flow Assay in a Parallel Plate Flow ChamberJohn T. Patton~GlycoTech Corporation, Rockville, Maryland 20850Flow assays allow visualization of c
Mario Roederer博士的團隊通過研究單細胞來更好地描述在HIV治療過程中免疫系統的特征。該團隊使用非人類靈長目模式動物，用單細胞基因表達分析提高T細胞的鑒定。他們使用了先進的技術，包括18色 FACS系統和Fluidigm BioMark? 系統進行單細胞基因表達分析。基本研究內容包括：