IsolationandlongtermcultivationofHumanCornealEndothelialCells
Coating of the Culture Dish SurfacesCulture dishes were incubated with a film of a 1:1 mixture of laminin (10 μg/ml and chondroitin sulfate (10 μg/ml) solubilized in selection medium at room temperature for 1-2 hr. The coating solution was aspirated and the dishes washed once with HBS.Subcultures of Human Corneal Endothelial Cells1. Corneas were removed from postmortem human eyes.2. Corneas were excis......閱讀全文
Isolation－and－longterm－cultivation－of－Human－Corneal－Endothelial－Cells
Coating of the Culture Dish SurfacesCulture dishes were incubated with a film of a 1:1 mixture of laminin (10 μg/ml and chondroitin sulfate (10 μg
Isolation－of－human－corneal－endothelial－cells－(HCECs)
Isolation of HCECs1.?The corneoscleral tissues were rinsed three times with primary cell culture system containing 50 mg/mL gentamicin and 1.25 mg
Isolation－and－cultivation－of－endothelial－progenitor－cells－(EPCs)
Circulating bone marrow (BM)–derived endothelial progenitor cells (EPCs) are recruited to the site of tissue regeneration and substantially contri
Isolation－of－lymphatic－endothelial－cells
Dermal Cell Suspensions??????????? 1.?Dermatomed 0.8-mm split-thickness skin was obtained from adult healthy individuals undergoing elective surgery.
Isolation－of－lymphatic－endothelial－cells
實驗概要This protocols provides a general protocol for isolation of lymphatic endothelial cells.實驗步驟Dermal Cell Suspensions1.?Dermatomed 0.8-mm split-thic
Human－thyroid－endothelial－cell－isolation
1.?Human thyroid glands were obtained at surgery from multinodular goiters. 2.?The tissue was incubated in 0.1% type II collagenase (Worthington) ov
PriCells:－Isolation－of－endothelial－progenitor－cells－(EPCs)
?PriCells: Isolation of endothelial progenitor cells (EPCs)??? 1.?Twenty-four mL venous blood was collected at each time point into Vacutainer CPT M
Isolation－and－characterization－of－rat－glomerular－endothelial－cells
Glomerular endothelial cells (GECs) from the kidney are in close juxtaposition to other cell types, such as mesangial cells, and may respond to as
Isolation－of－human－prostatic－epithelial－cells
1.?A small piece of tissue from each specimen was removed and minced. 2.?The tissue was digested with collagenase overnight. 3.?To remove the colla
Isolation－of－human－endometrial－epithelial－cells
Tissue collection1.?Endometrial biopsies were collected from women undergoing gynaecological procedures for benign conditions. 2.?All women reported
Isolation－of－Human－Pulmonary－Epithelial－Cells
?Isolation of Human Pulmonary Epithelial Cells????1.?Pulmonary epithelial cells from human lung tissue were isolated.2.?Lungs were perfused with 10
Isolation－of－human－atrial－or－ventricular－cells
1.?Human tissue was derived from atrial or ventricular biopsy specimens belonging to patients undergoing heart surgery.2.?Isolated myocardial tissue w
Isolation－and－culture－of－rat－coronary－microvascular－endothelial－cells
CMVE were isolated from Wistar rat hearts by digestion of primary cell isolation kit.1.?Hearts mounted on a Langendorff apparatus were perfused at 3
Isolation－of－human－prostatic－smooth－muscle－cells
Human prostate tissues 1.Human hyperplastic prostates were obtained during surgery from four men through transurethral resection of the prostate. 2.
Isolation－of－human－uterine－smooth－muscle－cells
1.?Approval to utilize human tissue was given by Hospital's Committee on Research Involving Human Subjects. 2.?Human myometrial and leiomyoma tissue
Isolation－and－Culture－of－Human－Liver－Stem－Cells
Materials and Methods 1.?Human hepatocytes were isolated from fresh surgical specimens of patients undergoing hepatectomies. Healthy liver tiss
Isolation－of－human－colonic－epithelial－cells－(EC)
1.Human colonic epithelial cells (EC) were isolated from freshly resected colonic surgical specimens obtained. 2.All diagnoses were confirmed by clini
Isolation－of－human－primary－gastric－mucosa－epithelial－cells
1.?A piece of gastric mucosa (～3 cm2) was obtained from the normal appearing mucosa of the stomach at surgery. 2.?The patients were proven to be no H.
Isolation－of－human－primary－ovarian－surface－epithelial－cells
1.?Surface epithelial cells from normal ovaries from surgical residual specimen were isolated using standard and Institutional Review (References)
Isolation－and－Culture－of－human－vascular－smooth－muscle－cells
1.?Human vascular smooth muscle cells?(SMCs) were isolated from human saphenous vein.2.?Small moistened pieces of tissue (6 mm2) were placed intima
Isolation－of－human－multipotent－mesenchymal－stem－cells－from－second
Isolation of human multipotent mesenchymal stem cells from second‐trimester amniotic fluid? Culture of MSC from amniotic fluid 1.?Twenty amniotic
Isolation－and－culture－of－human－pulmonary－artery－smooth－muscle－cells－...
Isolation and culture of human pulmonary artery smooth muscle cells (PASMCs) 1.?The human pulmonary arteries were opened to expose the endothelia
Isolation－of－stromal－vascular－cells－from－human－adipose－tissue
Stromal stem cells proliferate in vitro and may be differentiated along several lineages. The scarcity of stromal stem cells in tissues and the la
Keratocyte－isolation－(corneal－fibroblasts)
Corneal keratocytes (corneal fibroblasts) that this corneal layer is specialized fibroblasts residing in the stroma, representing about 85-90% of
Isolation－and－Culture－of－Multipotent－Stem－Cells－from－Human－Bone－Morrow
Bone marrow contains three types of stem cells:1.?Hematopoietic stem cells give rise to the three classes of blood cells that are found in the circu
Culture－of－retinal－endothelial－cells－and－pericytes
Isolation of retinal microvessels1.?Eyes were obtained and transported on ice.2.?Eyes were cut circumferentially 3 mm posterior to the limbus, the v
Peripheral－blood－“endothelial－progenitor－cells”
EPC Isolation and Characterization1.?EPCs were obtained by isolating mononuclear cells using Ficoll density-gradient centrifugation of human blood buf
Isolation－of－papillary－cells
Isolation of renal papillary cells1.?For isolation of papillary cells, kidneys were harvested and kept in HBSS containing 15 mM HEPES, penicillin/
Isolation－of－papillary－cells
實驗概要 This protocols provides a general protocol for isolation of papillary cells. 實驗步驟 Isolation of renal papillary cells 1.?For ?isolation of pa
細胞組織消化常用的幾種酶的選擇
直接從生物體獲取的組織，一般需要將其消化成單個細胞才能進行體外培養。這種直接從離體組織獲得的細胞，更接近于生物體內的生活狀態，且生物性狀尚未發生很大改變，因此在藥物篩選、細胞移植、類器官培養、腫瘤研究等眾多領域備受歡迎。但組織消化過程中常遇到多種問題，例如消化不完全、細胞死亡率高等。如何克服這些問題