Cultureofhumanrenalproximaltubulecellsandrenalcorticalfibroblasts
1. The method for primary culture of human renal proximal tubule cells (PTC) and renal cortical fibroblasts (CF) is described in detail.2. Renal cortical tissue was dissected from the medulla, minced, digested with collagenase, and passed through a 100-μm mesh.3. Filtered tissue was resuspended in 45% Percoll and separated into four distinct bands by isopyknic ultracentrifugation.4. The uppermos......閱讀全文
Culture－of－human－renal－proximal－tubule－cells－and－renal－cortical－fibroblasts
1.?The method for primary culture of human renal proximal tubule cells (PTC) and renal cortical fibroblasts (CF) is described in detail.2.?Renal cor
Culture－of－human－venous－smooth－muscle－cells
Culture of human venous smooth muscle cells???1.?Explants of the 2 groups of skins (control subjects and patients with varicose veins) were prepared
Isolation－and－Culture－of－Human－Liver－Stem－Cells
Materials and Methods1.?Human hepatocytes were isolated from fresh surgical specimens of patients undergoing hepatectomies. Healthy liver tissue (
Culture－of－Human－Embryonic－Stem－Cells－(hESC)
All cell lines are initially grown according to the supplier's protocols but we are adapting them to one simple protocol outlined below:6-well pla
Isolation－and－Culture－of－human－vascular－smooth－muscle－cells
1.?Human vascular smooth muscle cells?(SMCs) were isolated from human saphenous vein.2.?Small moistened pieces of tissue (6 mm2) were placed intima
Isolation－and－culture－of－human－pulmonary－artery－smooth－muscle－cells－...
Isolation and culture of human pulmonary artery smooth muscle cells (PASMCs)1.?The human pulmonary arteries were opened to expose the endothelial su
Derivation－and－Culture－of－Cortical－Astrocytes
實驗概要Astrocytes are the ?most numerous cell type in the central nervous system (CNS). They play ?critical roles in adult CNS homeostasis, provide bioch
免疫細胞表面抗原分子CD家族對照表(CD1CD247)3
CD7Membrane glycoprotein and Fc receptor for IgM?Homologous to TCR gamma, Ig kappaMembrane expression early during T ontogeny, before TCR rearrangemen
Isolation－and－Culture－of－Multipotent－Stem－Cells－from－Human－Bone－Morrow
Bone marrow contains three types of stem cells:1.?Hematopoietic stem cells give rise to the three classes of blood cells that are found in the circu
Ex－Vivo－Human－Primary－Mesenchymal－Stem－Cells－(MSCs)－Culture
Human bone marrow contains mesenchymal progenitors (mesenchymal stem cells, MSCs).? MSCs produce adventitial cells in the human bone marrow microenvir
Isolation,－Culture,－Characterization－of－Cortical－and－Hippocampal－Neurons
實驗概要The ?ability to culture primary neurons under serum-free conditions ?facilitates tighter control of neuronal studies. Some serum-free media ?and s
Isolation－and－culture－of－smooth－muscle－cell
1.Male Wistar rats of about 250g body wt were anesthetized with ether and were decapitated. ?2.Kidneys and thoracic aorta were removed, rinsed with
細胞組織消化常用的幾種酶的選擇
直接從生物體獲取的組織，一般需要將其消化成單個細胞才能進行體外培養。這種直接從離體組織獲得的細胞，更接近于生物體內的生活狀態，且生物性狀尚未發生很大改變，因此在藥物篩選、細胞移植、類器官培養、腫瘤研究等眾多領域備受歡迎。但組織消化過程中常遇到多種問題，例如消化不完全、細胞死亡率高等。如何克服這些問題
Culture－of－BEND－Cells－(Bovine－Endometrial－Cells)
Culture of BEND Cells (Bovine Endometrial Cells)Charles E. Krininger, III and Peter J. Hansen?Dept. of Animal Sciences, University of FloridaThis prot
Different－types－of－human－cells
Human primary cells are cells taken from living human beings and cultured.? These cells retain the differentiation of the original cells taken in
Human－B－cell－isolation－and－culture
B cell isolation1.?Donor blood was obtained with informed consent under guidelines issued by the Medical Ethics Committee. 2.?Mononuclear cell fractio
Human－B－cell－isolation－and－culture
實驗概要This protocol provides a general protocol for human B cell isolation and culture.實驗步驟B cell isolation1.?Donor blood was obtained with informed con
Culture－of－retinal－endothelial－cells－and－pericytes
Isolation of retinal microvessels1.?Eyes were obtained and transported on ice.2.?Eyes were cut circumferentially 3 mm posterior to the limbus, the v
Isolation－and－culture－of－porcine－muller－cells
1.?Porcine eyes were harvested from pentobarbital-anesthetized animals, each weighing 30 to 40 pounds, and transported to the laboratory in ice-co
Isolation－and－culture－of－pancreatic－stellate－cells
Pancreatic stellate cells (PaSCs or PSCs) are myofibroblast-like cells found in the areas of the pancreas that have exocrine function.? PaSCs are
Immunofluorescence－Microscopy－of－tissue－culture－cells
Immunofluorescence Microscopy of tissue culture cellsThese methods are written for direct staining of filamentous actin with bodipy FL-phallicidin and
Isolation,－Culture,－and－Differentiation－of－Progenitor－Cells
Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous SystemScott R. Hutton?and?Larysa H. Pevny1UNC Neuroscience Center
Culturing－Human－Neural－Stem－Cells
實驗概要Neural ?stem cells (NSC) are valuable resources because of their ability to ?differentiate into neurons and glial cells with applications in ?neur
Activation－and－Expansion－of－Human－Treg－Cells
實驗概要This protocol is intended for activation and expansion of human Treg cells isolated with the Dynal? CD4 CD25 Treg Kit (Cat. no. 113.23D). The exp
Isolation－of－Human－Pulmonary－Epithelial－Cells
?Isolation of Human Pulmonary Epithelial Cells????1.?Pulmonary epithelial cells from human lung tissue were isolated.2.?Lungs were perfused with 10
Isolation－of－human－atrial－or－ventricular－cells
1.?Human tissue was derived from atrial or ventricular biopsy specimens belonging to patients undergoing heart surgery.2.?Isolated myocardial tissue w
Isolation－of－human－endometrial－epithelial－cells
Tissue collection1.?Endometrial biopsies were collected from women undergoing gynaecological procedures for benign conditions. 2.?All women reported
Isolation－of－human－prostatic－epithelial－cells
1.?A small piece of tissue from each specimen was removed and minced. 2.?The tissue was digested with collagenase overnight. 3.?To remove the collagen
Purification－of－human－mononuclear－cells－and－neutrophils
PurposeMaterials10ml 6% dextran + 7ml citrate/citric acidDextran: T500 --> 6g+100ml PBSCitrate solution: 25g Na Citrate + 8g citric acid + 500 ml PBS4
Isolation－and－growth－of－mouse－primary－myoblasts
Isolation of limb muscle from neonatal mice1.?Neonatal mice by decapitation or CO2 inhalation.2.?Rinse the limbs with 70% ethanol and remove them wi