PurposeThe main purpose of the flow chamber assay is to visualize and measure interactions between flowing cells expressing a given adhesion molecule on their surface, and their receptor, either directly coated on the flow chamber lower wall or expressed on a cell monolayer.The present protocol will focus on the interactions between L-selectin expressed on neutrophils and PNAd coated onto the plastic surface.For neut......閱讀全文
德國WALZ公司生產的M系列調制葉綠素熒光成像系統IMAGING-PAM,作為全球最權威的葉綠素熒光成像系統,在國際科研界得到了廣泛應用,已經成為葉綠素熒光成像研究的行業標準。 澤泉科技有限公司作為德國WALZ在中國的技術服務中心,一直以來致力于推廣調制葉綠素
2013年5月16日-19日,由中國化學會主辦、廈門大學承辦、復旦大學、浙江大學協辦的為期四天的第八屆全國微全分析系統學術會議、第三屆全國微納尺度生物分離分析學術會議暨第五屆國際微化學與微系統學術會議在美麗的海濱城市廈門隆重召開。以下是
Instruments For ElectrophysiologyProducts include microelectrode, voltage and current clamp amplifiers, perfusion chambers, perfusion heating systems,
General AdvicePCR allows the production of more than 10 million copies of a target DNA sequence from only a few molecules. The sensitivity of this tec
聚甲基丙烯酸甲酯(PMMA)作為一種典型的食品接觸材料, 具有透光率高、 性能優良及價格適宜等優點, 被廣泛用于制作餐具、 外包裝盒等食品接觸制品. 當此類制品與食品接觸時, 其殘留的甲基丙烯酸甲酯單體可能遷移釋放到食品中. 甲基丙烯酸甲酯有一定的毒性, 可引起輕度皮炎和結膜炎, 并對人的神經系
General AdvicePCR allows the production of more than 10 million copies of a target DNA sequence from only a few molecules. The sensitivity of this tec
“香江學者計劃”學術委員會日前召開終評會議,2012年度”香江學者計劃”(以下簡稱”計劃”)共有64位內地優秀博士(名單見附件)獲得資助,將赴港與香港高等院校的導師開展為期兩年的合作研究。 ”計劃”在2011年首次資助44位內地優秀博士,已于今年3月
BASIC THEORY AND USE OF GC-MSbyDr. Eugenia SobolevaContent1. Introduction.2. GC-MS syste
美國BARTZ公司最早研制生產微根窗根系觀測儀器,BTC-100成為國際上應用最廣、發表論文最多的根系研究儀器技術,其學術研究論文涵蓋幾十種世界知名學術期刊和專著,包括Science、Nature、Global change biology(影響因子8.502)、New 
Scanning the human genome with combinatorial transcription factor librariesPublished online: 18 February 2003, doi:10.1038/nbt794March 2003 Volume 21
This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor
Experimental sectionChemicals and reagentsOne lot of PuregonR-HP of 50 IU/0.5mL and two lots of PuregonR-HP of 100 IU/0.5mL (rhFSH) (Organon, Oss, N
2011CJ Bernacchi, ADB Leakey, BA Kimball, DR Ort (2011) Growth of soybean at future tropospheric ozone concentrations decreases canopy evapotransp
Gating:Gating will allow you to view cells of interest by any combination of criteria that you choose. Gating does not change the intensity value assi
GE HealthcareBenzamidine Sepharose™ 6B is p-aminobenzamidine covalentlyattached to Sepharose 6B by the epoxy coupling method.p-Aminobenzamid
Reservations:Schedule time on a cytometer (FACS or Calibur) by filling in the calendar at the flow lab. Don't grossly overbook since we have to pa
TroubleshootingCritical Steps(1) Don’t trypsinize cells for too long when collecting them.(2) Rotation speed should be no more than 1500 rpm during ce
Use of Expired MaterialsExpired medical materials such as drugs, fluids and sutures may not be used on any research animal who is unanesthetized
ObjectiveThis experiment will seek to demonstrate regulative development in Ambystoma mexicanum embryos. Specifically, we will split the mor
Table 2: Binding sites and identity of ZFPs used in VEGF activationWe then generated artificial transcription factors by fusing the three-fi
FL3500雙調制葉綠素熒光儀 (新升級型號為FL6000) FL3500雙調制葉綠素熒光儀是專門用于對藍綠藻或綠藻等微藻,葉綠體或類囊體懸浮物,乃至葉片進行光合作用研究的強大科研工具。儀器具備雙通道測量控制,可控制測量樣品的溫度,并配備單翻轉光(STF),內置多種可用戶自行修改的測量程序
B: GLC procedureThe GLC will be prepared for your use. Please do not attempt to turn on the GLC on your own if it is off. Do not change any of the set
Spore Prep - Inoculating & HarvestingDescription A spore prep is a method of preserving a sporulating strain of Streptomyces. The stock is st
Homogeneity of cell populations is a prerequisite for the analysis of biochemical and molecular events during male gamete differentiation. Given the c
Carbohydrate Assay (Hancock Laboratory) (Accessible only by IE)This protocol is used to determine the relative amounts of LPS CHO present in a gi
The chicken is a classic organism used to illustrate the principles of basic embryology. One of the developmental systems which has been examined in g
Cell Adherence Assay (LTI)General and nice Protocol for cell adherence assay. Proteins are coated on microtiter plates and cells are added; after
IntroductionThere are two modes of development common to most species in the animal kingdom.Virtually all embryos undergo both mosaic and regulative d
Flow cell assays are very useful for studying microtubule motility, microtubule dynamics, kinetochore-microtubule interactions and action of severing/
Dynamic Flow Assay in a Parallel Plate Flow ChamberJohn T. Patton~GlycoTech Corporation, Rockville, Maryland 20850Flow assays allow visualization of c