IsolationandDifferentiationofAmnionDerivedStemCells(ADSCs)
ADSC isolation, culture, and cloningRat amnion membrane is mechanically separated from the chorion of embryonic day 18.5 Sprague–Dawley rat embryosThe tissue is washed extensively with phosphate-buffered saline (PBS) and subsequently cut into small piecesMembrane fragments is placed in six-well plastic tissue culture dishes in a minimal volume (0.5 ml) of Dulbecco's modified Eagle's medium (DMEM; Invitrogen, ......閱讀全文
Isolation－and－Differentiation－of－AmnionDerived－Stem－Cells－(ADSCs)
ADSC isolation, culture, and cloning Rat amnion membrane is mechanically separated from the chorion of embryonic day 18.5 Sprague–Dawley rat embry
Isolation,－Culture,－and－Differentiation－of－Progenitor－Cells
Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous System Scott R. Hutton ?and? Larysa H. Pevny1 UNC Neurosc
Isolation,－Culture,－and－Differentiation－of－Progenitor－Cells2
Slide Coating13. Just before use, dilute the poly-D-lysine stock solution?1:50 in H2O. Add 0.2 mL of diluted poly-D-lysine to each well?of an eight-we
Isolation－and－Culture－of－Human－Liver－Stem－Cells
Materials and Methods 1.?Human hepatocytes were isolated from fresh surgical specimens of patients undergoing hepatectomies. Healthy liver tiss
Isolation－of－human－multipotent－mesenchymal－stem－cells－from－second
Isolation of human multipotent mesenchymal stem cells from second‐trimester amniotic fluid? Culture of MSC from amniotic fluid 1.?Twenty amniotic
Isolation－and－Culture－of－Multipotent－Stem－Cells－from－Human－Bone－Morrow
Bone marrow contains three types of stem cells:1.?Hematopoietic stem cells give rise to the three classes of blood cells that are found in the circu
Isolation－of－papillary－cells
Isolation of renal papillary cells1.?For isolation of papillary cells, kidneys were harvested and kept in HBSS containing 15 mM HEPES, penicillin/
Isolation－of－papillary－cells
實驗概要 This protocols provides a general protocol for isolation of papillary cells. 實驗步驟 Isolation of renal papillary cells 1.?For ?isolation of pa
CD133－isolation,－expansion,－and－differentiation
1.?Renal progenitor cells were obtained from the normal portion of cortex obtained from surgically removed kidneys. 2.?After dissection and passage
Isolation－of－lymphatic－endothelial－cells
Dermal Cell Suspensions??????????? 1.?Dermatomed 0.8-mm split-thickness skin was obtained from adult healthy individuals undergoing elective surgery.
Isolation－of－lymphatic－endothelial－cells
實驗概要This protocols provides a general protocol for isolation of lymphatic endothelial cells.實驗步驟Dermal Cell Suspensions1.?Dermatomed 0.8-mm split-thic
Isolation－of－rodent－pancreatic－β－cells
1.?Adult rats weighing 250-350g were anesthetized, sacrificed and immediately used for pancreas sampling.2.?Rat islets were isolated from male wistar
Cryopreserving－Neural－Stem－Cells
實驗概要There ?are numerous protocols available for cryopreserving neural stem cells ?(NSCs) derived from human embryonic stem cells; the primary objectiv
Differentiating－Neural－Stem－Cells－into－Neurons－and－Glial－Cells
實驗概要The protocols in ?this section describe the steps involved in differentiating neural stem ?cells (NSC) to neurons, astrocytes, and oligodendrocyte
Isolation－of－human－endometrial－epithelial－cells
Tissue collection1.?Endometrial biopsies were collected from women undergoing gynaecological procedures for benign conditions. 2.?All women reported
Isolation－of－normal－mammary－epithelial－cells
1.?A small piece of tissue from each specimen was removed and minced. 2.?The tissue was digested with collagenase overnight. 3.?To remove the collagen
Isolation－of－Human－Pulmonary－Epithelial－Cells
?Isolation of Human Pulmonary Epithelial Cells????1.?Pulmonary epithelial cells from human lung tissue were isolated.2.?Lungs were perfused with 10
Isolation－of－human－atrial－or－ventricular－cells
1.?Human tissue was derived from atrial or ventricular biopsy specimens belonging to patients undergoing heart surgery.2.?Isolated myocardial tissue w
Isolation－and－culture－of－porcine－muller－cells
1.?Porcine eyes were harvested from pentobarbital-anesthetized animals, each weighing 30 to 40 pounds, and transported to the laboratory in ice-co
Isolation－and－culture－of－pancreatic－stellate－cells
Pancreatic stellate cells (PaSCs or PSCs) are myofibroblast-like cells found in the areas of the pancreas that have exocrine function.? PaSCs are
Isolation－of－human－prostatic－epithelial－cells
1.?A small piece of tissue from each specimen was removed and minced. 2.?The tissue was digested with collagenase overnight. 3.?To remove the collagen
Culturing－Human－Neural－Stem－Cells
實驗概要 Neural ?stem cells (NSC) are valuable resources because of their ability to ?differentiate into neurons and glial cells with applications in ?n
PriCells:－Isolation－of－endothelial－progenitor－cells－(EPCs)
?PriCells: Isolation of endothelial progenitor cells (EPCs)??? 1.?Twenty-four mL venous blood was collected at each time point into Vacutainer CPT M
Isolation－of－human－uterine－smooth－muscle－cells
1.?Approval to utilize human tissue was given by Hospital's Committee on Research Involving Human Subjects. 2.?Human myometrial and leiomyoma tissue
Isolation－and－characterization－of－rat－glomerular－endothelial－cells
Glomerular endothelial cells (GECs) from the kidney are in close juxtaposition to other cell types, such as mesangial cells, and may respond to as
Isolation－of－human－corneal－endothelial－cells－(HCECs)
Isolation of HCECs1.?The corneoscleral tissues were rinsed three times with primary cell culture system containing 50 mg/mL gentamicin and 1.25 mg
Isolation－and－cultivation－of－endothelial－progenitor－cells－(EPCs)
Circulating bone marrow (BM)–derived endothelial progenitor cells (EPCs) are recruited to the site of tissue regeneration and substantially contri
Isolation－of－human－colonic－epithelial－cells－(EC)
1.Human colonic epithelial cells (EC) were isolated from freshly resected colonic surgical specimens obtained. 2.All diagnoses were confirmed by clini
Isolation－of－human－prostatic－smooth－muscle－cells
Human prostate tissues1.Human hyperplastic prostates were obtained during surgery from four men through transurethral resection of the prostate. 2.All
Isolation－of－peripheral－blood－mononuclear－cells－(PBMC)
1.?Acid-citrate dextrose-treated blood (450 ml) was obtained from donors. 2.?PBMC were isolated by centrifugation over lymphocyte separation medium. 3