IsolationandDifferentiationofAmnionDerivedStemCells(ADSCs)
ADSC isolation, culture, and cloningRat amnion membrane is mechanically separated from the chorion of embryonic day 18.5 Sprague–Dawley rat embryosThe tissue is washed extensively with phosphate-buffered saline (PBS) and subsequently cut into small piecesMembrane fragments is placed in six-well plastic tissue culture dishes in a minimal volume (0.5 ml) of Dulbecco's modified Eagle's medium (DMEM; Invitrogen, ......閱讀全文
Culture－of－Human－Embryonic－Stem－Cells－(hESC)
All cell lines are initially grown according to the supplier's protocols but we are adapting them to one simple protocol outlined below: 6-well pl
Culturing－Rat－Fetal－Neural－Stem－Cells
實驗概要 Rat ?neural stem cells (NSCs) serve as a well-established model for ?investigating human brain development, disease processes, and treatment ?s
Stem－cell－characteristics－of－amniotic－epithelial－(AE)－cells
Isolation of AE Cells1.?Human placentae were obtained with the approval of the institutional review board, after uncomplicated elective caesarean
Isolation－and－Culture－of－Human－Brain－Tumor－Stem－Cell
The isolation, culture, identification, and purification of stem cells from primary human brain tumors of different phenotypes have marked capacit
ELECTROPORATION－OF－ES－CELLS－AND－ISOLATION－OF－H/R－CLONES
Need 1.5-2 x 107 cells from a 2 day culture. 1. Cells are harvested as normal, washed x 1 in PBS then taken up at conc. of 1.2 x 10 7 cells/ml in co
Isolation－of－human－primary－gastric－mucosa－epithelial－cells
1.?A piece of gastric mucosa (～3 cm2) was obtained from the normal appearing mucosa of the stomach at surgery. 2.?The patients were proven to be no H.
Isolation－of－human－primary－ovarian－surface－epithelial－cells
1.?Surface epithelial cells from normal ovaries from surgical residual specimen were isolated using standard and Institutional Review (References)
Isolation－and－Culture－of－human－vascular－smooth－muscle－cells
1.?Human vascular smooth muscle cells?(SMCs) were isolated from human saphenous vein.2.?Small moistened pieces of tissue (6 mm2) were placed intima
Isolation－and－culture－of－rat－coronary－microvascular－endothelial－cells
CMVE were isolated from Wistar rat hearts by digestion of primary cell isolation kit.1.?Hearts mounted on a Langendorff apparatus were perfused at 3
GentleMACS/單細胞懸液制備文獻集錦
? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?? ? ? ? ? ? 種屬 處理組織 文章名稱 human ? ? ? ? ? ? ?
Growing－feederindependent－embryonic－stem－cells§
We use feeder-independent ES cell lines derived from the 129/Ola strain of mice (Nichols et al., Development 110, p.1341, 1990). These cells are easy
Xenofree－Culture－of－Neural－Stem－Cells
實驗概要Neural ?stem cells (NSCs) derived from human embryonic stem cells (hESCs) have ?the potential to help provide understanding for human neurogenesis
Noninvasive－Human－Nuclear－Transfer－with－Embryonic－Stem－Cells
Noninvasive Human Nuclear Transfer with Embryonic Stem CellsSohyun L. McElroy1?and?Renee A. Reijo PeraCenter for Human Embryonic Stem Cell Research an
胚胎干細胞培養
Media and Solution required for ES Cell Culture?(Bowtell Lab)???Routine Culturing of ES Cells?(Bowtell Lab)??Routine Splitting and freezing of cells?(
Isolation－and－culture－of－human－pulmonary－artery－smooth－muscle－cells－...
Isolation and culture of human pulmonary artery smooth muscle cells (PASMCs) 1.?The human pulmonary arteries were opened to expose the endothelia
Isolation－of－T4－cells－from－xenograft－tumors
1 Put mouse down?2 Wash skin by dipping mice into ETOH?3 Remove tumor and put into 5ml PBS containing Fungizone and Penstrep?4 Swirl around to rinse t
Isolation－of－stromal－vascular－cells－from－human－adipose－tissue
Stromal stem cells proliferate in vitro and may be differentiated along several lineages. The scarcity of stromal stem cells in tissues and the la
STEM－CELLS：增壓干細胞創建新療法
近日，發表在國際期刊《Stem Cells》上的一項研究指出，阿德萊德大學的研究人員發現了一種培養干細胞的新方法，該方法可使細胞生長的更快，更強壯。 Kisha Sivanathan博士說這在干細胞上是一個激動人心的突破性研究。“成人間充質干細胞可以從體內許多組織包括骨髓中獲得，這種干細
Sleeping－Beauty－transposon－mutagenesis－in－rat－spermatogonial－stem－cells
Sleeping Beauty transposon mutagenesis in rat spermatogonial stem cells Zoltán Ivics,1, 2 Zsuzsanna Izsvák,1, 2 Gerardo Medrano,3, 4 Karen M Chapman3
Derivation－of－Dopaminergic－Neurons－(from－Human－Embryonic－Stem－Cells)
實驗概要Directed ?differentiation of specific lineages has been a focal point in the ?field of human embryonic stem cell (hESC) research. Cell replacement
《Stem－Cells》：胚胎干細胞長成軟骨
美國萊斯大學的生物醫學工程師發明了一項能夠利用人類干細胞生長出軟骨的新技術。這種方法能夠用于培養膝蓋、下巴、臀部和其他關節的軟骨替換。 由于自然的軟骨組織是不能夠自我痊愈的，所以研究人員長期以來都在尋找培養替代的軟骨的方法。培養的這種軟骨可用于修復軟骨損傷。這項研究為利用胚胎干細胞制造類軟骨細胞提
mice－islet－isolation
1.?Islets of Langerhans were isolated from 5- to 7-week-old nonobese diabetic (NOD) mice. 2.?Which involves cannulation of the common bile duct and
Isolation－and－longterm－cultivation－of－Human－Corneal－Endothelial－Cells
Coating of the Culture Dish SurfacesCulture dishes were incubated with a film of a 1:1 mixture of laminin (10 μg/ml and chondroitin sulfate (10 μg
Freezing－and－Thawing－of－MEFs
Author:?Shalini Jain and Hariom YadavAffiliation:?Animal Biochemistry Division, National Dairy Research Institute, Karnal-132001, Haryana, IndiaDate A
Magnetic－Depletion－of－SSEA4+－Undifferentiated－Embryonic－Stem－Cells
實驗概要Human ?embryonic stem cells can be differentiated into neural-, mesenchymal ?and hematopoetic stem cells. This product is intended for the magneti
Stem－Cells：癌癥干細胞的新調控機制
肺癌是一種非常普遍的人類惡性腫瘤，也是造成癌癥死亡的頭號殺手。肺癌主要分為小細胞肺癌（15%）和非小細胞肺癌（85%）兩大類，其中非小細胞肺癌與吸煙高度相關，而且生存率很低。近十年來肺癌發病率在中國攀升得很快，這主要是因為吸煙人數快速增加（大多是男性）。 許多科學家認為，完全治愈肺癌需要靶標癌
Ex－Vivo－Human－Primary－Mesenchymal－Stem－Cells－(MSCs)－Culture
Human bone marrow contains mesenchymal progenitors (mesenchymal stem cells, MSCs).? MSCs produce adventitial cells in the human bone marrow microenvir
Isolation－and－culture－of－SpragueDawley－rat－aortic－smooth－muscle－cells
The intact mature arterial media is composed of at least four phenotypically unique cell subpopulations that reside in distinct medial layers.? T
Isolation－of－Total－RNA－from－Animal－Cells－use－RNeasy－Mini－Kit
實驗概要Extract the total RNA from animal cells by using RNeasy Mini Kit (QIAGEN No.74104)?主要試劑SDS based extraction solution實驗步驟1. ?Harvest cells.1)?? Try
The－OP9DL1－System:－Generation－of－TLymphocytes－from－Embryonic1
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In VitroRoxanne Holmes?and?Juan Carlos Zú?iga-Pflücker1Sunn