WIKI資訊
Isolation of HCECs1. The corneoscleral tissues were rinsed three times with primary cell culture system containing 50 mg/mL gentamicin and 1.25 mg/mL amphotericin B. 2. The central cornea was removed by a trephine of 8-mm diameter. 3. Descemet’s membrane and corneal endothelial cells were stripped from the posterior surface of the peripheral corneoscleral tissue under a dissecting microscope.......閱讀全文
Coating of the Culture Dish SurfacesCulture dishes were incubated with a film of a 1:1 mixture of laminin (10 μg/ml and chondroitin sulfate (10 μg