GuidelinesfortheUseofAnalgesicsandTranquilizersinLaboratoryAnimal6
Anesthetic MachineThe best method of delivering an inhalant anesthetic is with an anesthetic machine. These machines precisely mix the gas with air or oxygen and can be easily adjusted. Machines can vary in construction and design. Anesthetic machines typically require more training to learn to operate. Anesthetic concentration is accomplished by sets of mixing valves or a precision vaporizer. Vaporiz......閱讀全文
Guidelines－for－theUse－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal5
Anesthetic Induction and MaintenanceInjectable AnesthesiaAnesthetic induction using injectable anesthetics is fairly simple. It involves admininsistra
Guidelines－for－theUse－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal2
General Some of the drugs listed here do not possess all three criteria for an anesthetic and must be used in combinations to achieve full anesthesi
Guidelines－for－theUse－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal4
Other?Analgesics Analgesics are pain relievers most often given after a surgery. Narcotic analgesics have already been described above. Nonsteroidal
Guidelines－for－theUse－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal6
?Anesthetic?MachineThe best method of delivering an inhalant anesthetic is with an anesthetic machine. These machines precisely mix the gas with air o
Guidelines－for－theUse－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal3
BarbituratesThe barbiturates are an acid ring molecule with various ring substitutes that imbue the drug with different properties. Barbiturates are a
Guidelines－for－theUse－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal7
ThermoregulationAnimals frequently become hypothermic during anesthesia because of inhalation of cold gases, exposure of body cavities to the room air
Guidelines－for－the－Use－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal
What is Anesthesia??Anesthesia is a state of unconsciousness induced in an animal. The three components of anesthesia are?analgesia?(pain relief),?amn
Guidelines－for－Retroorbital－Bleeding－in－Laboratory－Rats－and－Mice
Materials Needed:Micro pipettes (l00 μl) or Pasteur pipettes drawn to fine tip2X2 gauze squaresNon-sterile glovesEppendorf or other tubes to hold samp
Yale－University－Policy－for－Aseptic－Amphibian－Survival－Surgery
Background:?Aseptic surgery by definition is surgery performed without contamination or exposure to pathogens. According to the AWR's and the?Guid
siRNA－Design－Guidelines
Using siRNA for gene silencing is a rapidly evolving tool in molecular biology. There are several methods for preparing siRNA, such as chemical synthe
General－Laboratory－Methods－for－Tetrapyrroles
There are thousands of porphyrins and metalloporphyrins, and hundreds of new derivatives appear each year. This variety arises because the cyclic
Long－PCR－Reagents－and－Guidelines
George Church Lab, Harvard Medical SchoolPCR_protocol.html">http://twod.med.harvard.edu/labgc/estep/longPCR_protocol.htmlEfficient Long PCR results fr
ELISA－Protocol－(General－Guidelines)
實驗概要Sandwich ?enzyme-linked immunosorbent assays (ELISAs) involve attachment of a ?capture antibody to a solid phase support. Samples containing known
ELISA－Protocol－(General－Guidelines)
實驗概要Sandwich ?enzyme-linked immunosorbent assays (ELISAs) involve attachment of a ?capture antibody to a solid phase support. Samples containing known
LongPCR－Reagents－and－Guidelines
Long-PCR Reagents and Guidelinesfrom George Church as Modified from Cheng et al. (1)General Guidelines for Long-PCR Conditions and Enzyme Mixtures====
Guidelines－for－Aseptic－Rodent－Survival－Surgery
Introduction:Aseptic surgery is surgery performed without contamination or exposure to pathogens. These policies and guidelines are provided to help e
RNase－and－DEPC－Treatment:－Fact－or－Laboratory－Myth
Researchers are usually trained in RNA isolation and analysis methods by one another or by technical manuals. Experimental procedures are often not qu
General－Laboratory－Procedures,－Equipment－Use,－and－Safety－Considerations
A. Storage . The following properties of reagents and conditions are important considerations in processing and storing DNA and RNA. Heavy metals pr
Apoptosis:－A－Laboratory－Manual－of－Experimental－Methods－Andrea－Cossarizza
THE CELL?1.?Morphological aspects of apoptosis?Walter Malorni, Stefano Fais & Carla Fiorentini?2.?Cell cycle?Miriam Capri & Daniela BarbieriTHE NUCLEU
ICBR－Flow－Cytometry－Core－Laboratory－Paraformaldehyde－Fixation－of－Cells
Background This fixation method is good for cells labelled by fluorochrome-conjugated antibodies to membrane antigens. It will stabilize the light s
Laboratory－Investigation：新藥可預防致命核輻射
新研究發現對抗致命的輻射有了新突破。在核輻射暴露后24小時內，一種再生肽可以幫助顯著提高小鼠的生存時間。目前這項研究出版在自然出版集團的雜志《Laboratory Investigation》上。 德州大學醫學分部研究作者Carla Kantara是生物化學和分子生物學博士后，他說當小鼠處于致
SMEAR－PREPARATION
The preparation of a smear is required for many laboratory procedures, including the Gram-stain. The purpose of making a smear is to fix the bacteria
The－iPhyClassifier,－an－Interactive－Online－Tool－for－Phytoplasma－...
The i PhyClassifier is an internet-based research tool for quick identification and classification of diverse phytoplasmas. The i PhyClassifier simu
High－Throughput－Isolation－Of－PCR－Products－Using－ChargeSwitch?－PCR－CleanUp
實驗概要The ChargeSwitch? ?PCR Clean-Up Kit allows rapid and efficient purification of PCR ?products from salts, primers, dNTPs, and other non-nucleic aci
Sterile－Technique
Good sterile technique is the first and most important step in insuring consistent results when employing recombinant DNA and protein expression techn
無菌化技術
Sterile TechniqueGood sterile technique is the first and most important step in insuring consistent results when employing recombinant DNA and protein
The－Impact－of－Harmo...－（一）
實驗概要During more than ?25 years of application in immunological sciences, ELISPOT has been ?established as a routine, robust, versatile, and reliable a
檢驗醫學中臨床應用準則的編寫要求
1.前言 臨床應用準則(簡稱準則)有助于臨床工作者對患者的診斷、治療和預后做出恰當和有效的決定。面對影響臨床結果的各種復雜因素，在決定是否采取必要的干預措施時．如有一個完善的臨床應用準則將是有幫助和有價值的。因為臨床應用準則有助于對患者做出正確的判斷，對診療工作提出符合實際情況的、最恰當的建議
通過可控制的微波加熱進行快速固相多肽合成
Rapid solid-phase peptide synthesis using thermal and controlled microwave irradiation Abstract: A rapid and efficient microwave-assisted solid-phase
病毒冷凍保藏技術
實驗概要Snap freezing, or flash freezing, is the process by which samples are lowered to temperatures below -70°C very rapidly using dry ice or liquid