Cultureofretinalendothelialcellsandpericytes
Isolation of retinal microvessels1. Eyes were obtained and transported on ice.2. Eyes were cut circumferentially 3 mm posterior to the limbus, the vitreous humor was removed, and the retina was exposed and transferred to solution of primary cell culture medium.3. Retinas were homogenized by two gentle up/down strokes in a 15 ml Dounce homogenizer (type A pestle).4. The homogenate was filtered ......閱讀全文
Culture－of－retinal－endothelial－cells－and－pericytes
Isolation of retinal microvessels1.?Eyes were obtained and transported on ice.2.?Eyes were cut circumferentially 3 mm posterior to the limbus, the v
Isolation－and－culture－of－rat－coronary－microvascular－endothelial－cells
CMVE were isolated from Wistar rat hearts by digestion of primary cell isolation kit.1.?Hearts mounted on a Langendorff apparatus were perfused at 3
Isolation－of－lymphatic－endothelial－cells
Dermal Cell Suspensions??????????? 1.?Dermatomed 0.8-mm split-thickness skin was obtained from adult healthy individuals undergoing elective surgery.
Isolation－of－lymphatic－endothelial－cells
實驗概要This protocols provides a general protocol for isolation of lymphatic endothelial cells.實驗步驟Dermal Cell Suspensions1.?Dermatomed 0.8-mm split-thic
Peripheral－blood－“endothelial－progenitor－cells”
EPC Isolation and Characterization1.?EPCs were obtained by isolating mononuclear cells using Ficoll density-gradient centrifugation of human blood buf
Culture－of－BEND－Cells－(Bovine－Endometrial－Cells)
Culture of BEND Cells (Bovine Endometrial Cells) Charles E. Krininger, III and Peter J. Hansen? Dept. of Animal Sciences, University of Florida Thi
PriCells:－Isolation－of－endothelial－progenitor－cells－(EPCs)
?PriCells: Isolation of endothelial progenitor cells (EPCs)??? 1.?Twenty-four mL venous blood was collected at each time point into Vacutainer CPT M
ex－vivo－expanded－endothelial－progenitor－cells
Cell Culture. 1.?Total hPBMCs were isolated from blood of human volunteers by density gradient centrifugation. 2.?Cells were plated on culture dishes
Isolation－and－characterization－of－rat－glomerular－endothelial－cells
Glomerular endothelial cells (GECs) from the kidney are in close juxtaposition to other cell types, such as mesangial cells, and may respond to as
Isolation－and－cultivation－of－endothelial－progenitor－cells－(EPCs)
Circulating bone marrow (BM)–derived endothelial progenitor cells (EPCs) are recruited to the site of tissue regeneration and substantially contri
Isolation－of－human－corneal－endothelial－cells－(HCECs)
Isolation of HCECs1.?The corneoscleral tissues were rinsed three times with primary cell culture system containing 50 mg/mL gentamicin and 1.25 mg
Isolation,－Culture,－and－Differentiation－of－Progenitor－Cells
Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous System Scott R. Hutton ?and? Larysa H. Pevny1 UNC Neurosc
Isolation－and－culture－of－porcine－muller－cells
1.?Porcine eyes were harvested from pentobarbital-anesthetized animals, each weighing 30 to 40 pounds, and transported to the laboratory in ice-co
Immunofluorescence－Microscopy－of－tissue－culture－cells
Immunofluorescence Microscopy of tissue culture cellsThese methods are written for direct staining of filamentous actin with bodipy FL-phallicidin and
Isolation－and－culture－of－pancreatic－stellate－cells
Pancreatic stellate cells (PaSCs or PSCs) are myofibroblast-like cells found in the areas of the pancreas that have exocrine function.? PaSCs are
Tissue－Culture－of－PtK1－cells
Please note: lately (2005 - present), we normally culture PtK1 cells in F-12 media, but they also grow in other types of media as described below.
Culture－of－Human－Embryonic－Stem－Cells－(hESC)
All cell lines are initially grown according to the supplier's protocols but we are adapting them to one simple protocol outlined below: 6-well pl
Eccles:Protein－Lysates－from－Cells－in－Culture
Cell Lysis Buffer 5mL 0.1M Tris HCl pH 8 (10mM) 0.44g NaCl (150mM) 0.02g EDTA (1mM) 0.5mL nonidet P40 (1% w/v) 0.05g SDS (0.1% w/v) Make up to 50mL
Isolation－and－Culture－of－Human－Liver－Stem－Cells
Materials and Methods 1.?Human hepatocytes were isolated from fresh surgical specimens of patients undergoing hepatectomies. Healthy liver tiss
Culture－of－human－venous－smooth－muscle－cells
Culture of human venous smooth muscle cells???1.?Explants of the 2 groups of skins (control subjects and patients with varicose veins) were prepared
Isolation－and－longterm－cultivation－of－Human－Corneal－Endothelial－Cells
Coating of the Culture Dish SurfacesCulture dishes were incubated with a film of a 1:1 mixture of laminin (10 μg/ml and chondroitin sulfate (10 μg
Isolating－Xenograft－Tumor－Cells－for－Tissue－Culture－or－Transplantation
Isolation of T4 cells from xenograft tumors ? Put mouse down Wash skin by dipping mice into ETOH Remove tumor and put into 5ml PBS containi
Isolation－and－Culture－of－human－vascular－smooth－muscle－cells
1.?Human vascular smooth muscle cells?(SMCs) were isolated from human saphenous vein.2.?Small moistened pieces of tissue (6 mm2) were placed intima
Primary－Culture－of－Pulmonary－Arterial－Smooth－Muscle－Cells
Primary Culture of Pulmonary Arterial Smooth Muscle Cells?1.Primary cultures of Pulmonary Arterial Smooth Muscle Cells (PASMCs) were isolated from hum
Isolation,－Culture,－and－Differentiation－of－Progenitor－Cells2
Slide Coating13. Just before use, dilute the poly-D-lysine stock solution?1:50 in H2O. Add 0.2 mL of diluted poly-D-lysine to each well?of an eight-we
Xenofree－Culture－of－Neural－Stem－Cells
實驗概要Neural ?stem cells (NSCs) derived from human embryonic stem cells (hESCs) have ?the potential to help provide understanding for human neurogenesis
Isolation－and－culture－of－human－pulmonary－artery－smooth－muscle－cells－...
Isolation and culture of human pulmonary artery smooth muscle cells (PASMCs) 1.?The human pulmonary arteries were opened to expose the endothelia
Live－imaging－with－Drosophila－tissue－culture－cells1
Introduction Live imaging provides an important complementation to the "snapshot" view obtained in fixed tissue by immunofluorescence. It allows fol
Live－imaging－with－Drosophila－tissue－culture－cells2
Materials & ReagentsDrosophila?Schneider S2 cellsSchneiders Medium (GIBCO/Invitrogen), 10% fetal calf serum, Antibiotics (Sigma A5955)Depression slide
Ex－Vivo－Human－Primary－Mesenchymal－Stem－Cells－(MSCs)－Culture
Human bone marrow contains mesenchymal progenitors (mesenchymal stem cells, MSCs).? MSCs produce adventitial cells in the human bone marrow microenvi