PrinciplesofAsepticTechnique——4
P REPARATION OF THE SURGEONIn a laboratory setting, the extent of surgeon preparation will depend on the facilities and the need for strict attention to aseptic technique. Well-equipped surgical facilities, in which sophisticated survival proce dures are performed, generally require surgeons to wear appropriate surgical clothing and to scrub, gown and glove. Instruction in such procedures should be done on a one-to-o......閱讀全文
Principles－of－Aseptic－Technique
INTRODUCTIONThe regulations promulgated to implement the amended Animal Welfare Act require that all survival surgery be performed using aseptic proce
Principles－of－Aseptic－Technique——3
PREPARATION OF THE ANIMAL The animals should be prepared in a n area separate from where surgery will be performed. Preparation is facilitated by fi
Principles－of－Aseptic－Technique——4
P REPARATION OF THE SURGEONIn a laboratory setting, the extent of surgeon preparation will depend on the facilities and the need for strict attention
Principles－of－Aseptic－Technique——2
PersonnelAseptic technique requires careful attention to a series of steps which begins with patient and instrument preparation and ends at final woun
Principles－of－Aseptic－Technique——3
PREPARATION OF THE ANIMALThe animals should be prepared in a n area separate from where surgery will be performed. Preparation is facilitated by first
Aseptic－Technique－and－Good－Cell－Culture－Practice
AimTo ensure all cell culture procedures are performed to a standard that will prevent contamination from bacteria, fungi and mycoplasma and cross con
Asepsis－and－Aseptic－Practices－in－the－Operating－Room
Preventing surgical site infection in the operating room is the primary goal of the surgical team, and all activities performed by the team support th
基本無菌化技術
INTRODUCTION The regulations promulgated to implement the amended Animal Welfare Act require that all survival surgery be performed using aseptic pr
Asepsis－and－Aseptic－Practices－in－the－Operating－Room——2
Principle #4All items introduced onto a sterile field should be opened, dispensed, and transferred by methods that maintain sterility and integrity.2,
基本無菌化技術4
P REPARATION OF THE SURGEONIn a laboratory setting, the extent of surgeon preparation will depend on the facilities and the need for strict attention
Aseptic－Techniques
Aseptic techniques ensure that all cell culture procedures are performed to a standard that will prevent contamination from bacteria, fungi, mycoplas
Principles－of－nucleic－acid－hybridization
Principles of nucleic acid hybridization5.2.1.?Nucleic acid hybridization is a method for identifying closely related nucleic acid molecules within tw
Immunofluroescence－Technique
Fix cells in 2% formaldehyde in PBS/pH 7.4 for 15 min. at 20oC. 2% formaldehyde is made up fresh prior to use by dissolving the appropriate amount
Sterile－Technique
Good sterile technique is the first and most important step in insuring consistent results when employing recombinant DNA and protein expression techn
Guidelines－for－Aseptic－Rodent－Survival－Surgery
Introduction:Aseptic surgery is surgery performed without contamination or exposure to pathogens. These policies and guidelines are provided to help e
ALKALINE－PHOSPHATASE－(APAAP)－TECHNIQUE
Preparation: Cytological PreparationsFixation: Air dry films or cytospin preparations overnight at room temperature. For frozen and paraffin sections
Yale－University－Policy－for－Aseptic－Amphibian－Survival－Surgery
Background:?Aseptic surgery by definition is surgery performed without contamination or exposure to pathogens. According to the AWR's and the?Guid
In－Vitro－Conservation－and－Cryopreservation－of－Ornamental－Plants
Today, the conservation of ornamental germplasm can take advantage of innovative techniques which allow preservation in vitro (slow growth storage
顯微操作技術（micromanipulation－technique）
顯微操作技術（micromanipulation technique）是指在高倍復式顯微鏡下，利用顯微操作器（micromanipulator）進行細胞或早期胚胎操作的一種方法。顯微操作器是用以控制顯微注射針在顯微鏡視野內移動的機械裝置。顯微操作的基礎平臺--倒置研究級顯微鏡（例如OLYMPUS的I
免疫印跡技術(Immunoblotting－technique)
免疫印跡技術又稱蛋白質印跡(Western blotting)是一種借助抗原鑒定特異性抗體的有效方法，該法是在凝膠電泳和固相免疫測定技術基礎上發展起來的一種新的免疫生化技術。本實驗以檢測可提取性核抗原(ENA)抗體為例。【實驗原理】先將ENA混合抗原經SDS-PAGE電泳，使各種抗原成分根據分子量不
沉淀反應技術(Precipitation－reaction－technique)
一、???? 概述?可溶性抗原（如細菌浸出液、含菌病料浸出液、血清以及其他來源的蛋白質、多糖質、類脂體等）與其相應的抗體相遇后，在電解質參與下，抗原抗體結合形成白色絮狀沉淀，出現白色沉淀線，此種現象稱為沉淀反應。沉淀反應中的抗原叫沉淀原（precipitinogen）,與沉淀原發生反應的抗體稱為沉淀
細胞培養實驗的無菌化技術
The use of aseptic technique is essential for avoiding the production of infection whilst undertaking tissue culture activities. Many activities tak
沉淀反應技術(Precipitation－reaction－technique)（2）
2．取試管5支（5mm×50mm）置于試管架上，編號。第1、2試管內加炭疽沉淀血清，第3、4試管內加正常血清，第5管內加待檢抗原，分別用毛細滴管加至4mm～5mm。3．第1、4、5試管輕輕疊加等量緩沖液，第2、3試管輕輕疊加等量待檢抗原。為防止上下兩界面破壞，可將小試管從試管架取出，微傾斜，沿試管壁
層析技術(Layeranalise－technique)
離子交換層析技術是以離子交換纖維素或以離子交換葡聚糖凝膠為固定相，以蛋白質等樣品為移動相，分離和提純蛋白質、核酸、酶、激素和多糖等的一項技術。（一）原理在纖維素與葡聚糖分子上結合有一定的離子基團，當結合陽離子基團時，可換出陰離子，則稱為陰離子交換劑。如二乙氨乙基（Dicthylaminoethyl，
免疫熒光技術(immunofluorescence－technique)2
實驗材料 1. 40 孔酶標板 ? 2. 1:300 乙肝表面抗原溶液 ? 3. 1:10 待測血清 ? 4. 健康人血清 ? 5. HBsAg 診斷血清 ? 6. 辣根過氧化物酶標記羊抗人 IgG 抗體（酶標二抗） ? 7. 抗原稀釋液（ pH9
免疫熒光技術(immunofluorescent－technique)簡介
1、熒光免疫測定技術的概念將試劑抗原或試劑抗體用熒光素進行標記，試劑與標本中相應的抗體或抗原反應后，測定復合物中的熒光素，這種免疫技術，稱為免疫熒光素技術。2、技術分類（1）熒光抗體技術（熒光顯微鏡技術）：抗原抗體反應后，利用熒光顯微鏡判定結果的檢測方法。（2）免疫熒光測定技術：抗原抗體反應后，利用
沉淀反應技術(Precipitation－reaction－technique)（1）
一、 概述可溶性抗原（如細菌浸出液、含菌病料浸出液、血清以及其他來源的蛋白質、多糖質、類脂體等）與其相應的抗體相遇后，在電解質參與下，抗原抗體結合形成白色絮狀沉淀，出現白色沉淀線，此種現象稱為沉淀反應。沉淀反應中的抗原叫沉淀原（precipitinogen）,與沉淀原發生反應的抗體稱為沉淀素（pre
免疫熒光技術(immunofluorescence－technique)3
思考題 1. 酶聯免疫吸附實驗的基本原理是什么？常用方法有那些？ ? 2. 間接法和直接法相比各有什么優缺點？ ? 放射免疫測定法—— 125 I 標記技術 放射免疫測定 (radioimmunoassay, RIA) 是將同位分析的高靈敏度與抗原抗體反應的特異性相結合，
免疫熒光技術(immunofluorescence－technique)1
免疫熒光技術(immunofluorescence technique)是一種以熒光物作為標記物的免疫分析技術，熒光物質分子在特定條件下吸收激發光的能量后，分子呈激發態而極不穩定，其迅速回到基態時，可以電磁輻射形式釋放出所有的光能，發射出波長較照射光長的熒光。用熒光素與已知的抗體（或抗原，較少用
細胞培養常規操作
常規操作(主要內容如下)·?????????Aseptic Technique·?????????Culture Vessels·?????????Cell Counting·?????????Primary Culture·?????????Maintenance of Cell Line?·??