Objective: to separate the Blastomere at different points in development and observe the growth of the resulting embryos.Procedure: We extractrf the gametes from the sea urchins, fertilized the sea urchin eggs, and removed the fertilization envelope. The cells were then be allowed to cleave, at which point they were be introduced into a hypertonic seawater solution. This caused the cells to shrink and break apar......閱讀全文
ObjectiveThe purpose of this experiment is to determine the effects of calcium-free seawater on the development of sea urchin embryos. Embryos were pl
Objective: This experiment aims to discover if temperature has an effect on the rates of sea urchin embryonic development. By altering the extern
ObjectiveTo observe the role sulfate plays in sea urchin gastrulation, and to replicate the findings of Karp and Solursh, that sea urchin embryos fail
PurposeThis experiment will study effects of lithium chloride on sea urchin development, forcusing on archenteron formation.IntroductionGastrulation i
Jill K. Flemming, Franklin & Marshall College, Class of 2001IntroductionThe objective of this project is to observe the effects of UV radiation on
Objective:To test the effects of UV radiation at 254nm on Sea Urchin embryo development after radiation at the 2-cell stage.Background:Sea Urchins exh
The Effects of NiCl2on Spicule FormationJessica Ann Billet, Franklin and Marshall, Class of 2000Background and ObjectiveSea urchins exhibit radial hol
Introduction The most important aspect of our cloning vectors is that t
Objective: to test the effects of inhibiting the fast and slow blocks to polyspermy on sea urchin development.BackgroundSea urchin eggs release compon
AbstractPrevious studies by Stachel and colleagues indicate that lithium chloride induction of post-midblastular Brachydanio rerio embryos r
FL3500雙調制葉綠素熒光儀 (新升級型號為FL6000) FL3500雙調制葉綠素熒光儀是專門用于對藍綠藻或綠藻等微藻,葉綠體或類囊體懸浮物,乃至葉片進行光合作用研究的強大科研工具。儀器具備雙通道測量控制,可控制測量樣品的溫度,并配備單翻轉光(STF),內置多種可用戶自行修改的測量程序
The following is written to help non-marine biologists use sea urchins occasionally in teaching; disregard if you have running sea water tanks in your
提要 采用靜電離子色譜法(EIC), 在ODS載體上涂覆膽汁酸誘導體膠束(CHAPS)進行陰離子和陽離子的同時分離. 以示差析光檢測器檢測, 分別以純水、 碳酸鹽、 磷酸鹽和十二烷基磺酸鹽電解質溶液為流動相, 探討Na2SO4和NaBr, Na2S2O3, NaF和NaNO3, NaNO3和KNO3
Spawning can be induced in adult sea urchinsby injecting 1 ml of a0.5M KCl solution into several sites in the soft membrane around the mouth. Wit
Histochemical staining of sea urchin embryos for alkaline phosphatase (AP) enzyme activity1. Obtain embryo samples, tube of AP substrate buffer&n
Gametes are collected as described. To remove some of their jelly coat,wash eggs several times in ASW by allowing them to settle and gently
PURPOSE: The objective of this experiment is to study the effect of lithium chloride treatment on early blastula xenopus embryos.INTRODUCTIO
日前,頂尖學術期刊《細胞》雜志推出2020年度最佳論文(Best of 2020)特刊,精選了過去一年里最為值得關注的幾項生物學研究。其中既包括了引人關注的新冠病毒研究,也有在腫瘤免疫療法、自閉癥神經生物學、AI藥物發現等領域令人振奮的前沿進展。在今天的這篇文章中,學術經緯將為讀者朋友們一起分享
Invertebrate embryos develop at different rates depending on the temperature of incubation. Descriptive stages based on the number of cells and their
Manipulating and analyzing DNA are fundamentals in the field of molecular biology. Indeed, separating complex mixtures of DNA into different sized fra
尊敬的各位同行: 第2 屆大連國際色譜學術報告會及儀器展覽會包含第37屆國際高效液相色譜及相關技術會議(含儀器展覽會) (HPLC 2011 Dalian) 及第18屆全國色譜學術報告會及儀器展覽會(18th NSEC),將于2011年10月8-11日在大連召開。 為使更多色譜工作者
Sample preparation and solubilization are crucial factors for the overall performance of the 2-D PAGE technique. Protein complexes and aggregates shou
MgCl2 concentrationRelationship between MgCl2 and dNTP concentrationdNTP concentrations of about 200μM each are usually recommended for the
前沿的學術交流,強大的演講嘉賓陣容,豐富的內容涵蓋。一個絕對不容錯過的知識盛宴——等你而來! 【嘉賓介紹】 Life Science Product Sales, Marketing & Business Development in Asia Pacific Dealers, D
A. Phenol extraction of DNA samplesPhenol extraction is a common technique used to purify a DNA sample (1). Typically, an equal volume of TE-saturated
2010年10月19日, 為期兩天的2010年微納尺度分離和分析技術學術會議暨第六屆全國微全分析學術會議在上海復旦大學復宣大酒店圓滿閉幕。大會閉幕前,多位國內著名專家學者做了大會報告,同大家一起探討了微全分析領域當前的發展趨勢和最新研究成果。以下是大會報告詳細內容。
Electrophoresis through agarose or polyacrylamide gels is the standard way to separate, identify and purify nucleic acid fragments. The location of th
Homogeneity of cell populations is a prerequisite for the analysis of biochemical and molecular events during male gamete differentiation. Given the c
Section 2.1Nondenaturing Polyacrylamide Gel Electrophoresis of ProteinsJohn M. Walker1. IntroductionSDS-PAGE (Section 2.2) is probably the most
Thin layer chromatography is based on the separation of a mixture of compounds as it migrates with the help of a suitable solvent through a thin layer