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Small DNA-probes from cosmids or plasmids clonesThese kind of probes, especially plasmids, have become out of fashion for 3D-FISH due to their delicate hybridization signals and the lack of a systematic compilation in databases. They are used only in special situations.We strongly recommend preparing these probes by genomic DNA nick-translation, if not done by sequence-specific primer pairs. Amplification and/or labe......閱讀全文
IntroductionMulticolour 3D-FISH in combination with confocal microscopy, 3D image reconstruction and quantitative image analysis is an efficient tool
Background:Zebrafish,or the teleost fish Danio rerio,is a rapidly developing organism that is apopular species for studying vertebrate development. Cl
Back to topReviewer CommentsReviewed by: Luis Antonio Parada, CIC Biogune, Derio, Spain.In my experience the primers are better preserved when kept at
PROPIDIUM IODIDE: The most commonly used dye for DNA content/cell cycle analysis is PROPIDIUM IODIDE (PI). It can be used to stain whole cells or isol
WHENVIRUSESINFECTEUKARYOTICCELLSdomly integrate into host genomes, double-stranded RNA (dsRNA) is frequently produced from the invading genes, either
Gastrulation has been celled "the most important time of your life" (Lewis Wolpert); without it you would be flat like a pancake! Vertebrate
DescriptionProtocol for intracytoplasmic staining of cytokines for FACS analysis Procedure1) Prepare spleen, lymph node or T cell clone cells as
Author NotesAfter the fourth round of DOP amplification the probe quality is considerably reduced.Use the low stringency cycles only in case you start
Sleeping Beauty transposon mutagenesis in rat spermatogonial stem cellsZoltán Ivics,1, 2 Zsuzsanna Izsvák,1, 2 Gerardo Medrano,3, 4 Karen M Chapman3,
Genomic DNA libraries Size of some genomes and chromosomes:Comparative Sequence Sizes(Bases)(yeast chromosome 3)350 ThousandEscherichia coli (bac
前言今年5月，David R. Liu教授與張鋒教授等人聯合創立Beam Therapeutics公司，再一次將“單堿基編輯技術”送上了熱搜榜，趁著熱度還未散去，我們繼上一期“單堿基編輯技術淺談”后，又為大家準備了一篇超級實用性的深度分析，帶你一起看看單堿基編輯的前世今生及如何一步步發展壯大的歷程！
二、DNA標記與細胞譜系示蹤發育生物學的重點包括構成器官或生物體的細胞類型的多樣性以及這些細胞的發育譜系歷史。這些方面通常作為一個方向被單獨研究，但最近的四篇新論文報道了一種將單細胞RNA測序（scRNA-seq）技術與基于CRISPR的譜系示蹤技術相結合來同時解剖轉錄組細胞表型和譜系歷史的方法。近
In Vivo Imaging of Far-red Fluorescent Proteins after DNA Electrotransfer to Muscle TissueDNA electrotransfer to muscle tissue yields long-term, high
In most animal species, the anteroposterior body axis is generated by the formation of repeated structures called segments. In vertebrate segmentation
BTX特殊電極的應用此次只將貼壁轉染、活體等進行總結，對于大規模的轉基因的應用，由于BTX公司有新的發展，故此次未將其列入，待下次完善后補充。1、Adherent Cell Transfections (ACT)貼壁細胞轉染?In-situ electroporation of cells in t
In Vitro Production of Bovine Embryos (P.J. Hansen Lab, Dept. of Animal Sciences, University of Florida)This protocol describe procedures for in
Mammalian RNA InterferenceThomas TuschlLaboratory for RNA Molecular BiologyThe Rockefeller University, New York Excerpted from RNAi: A Guide
Metabolic biotinylation of mammalian cell receptors for imagingBakhos A. Tannous , btannous@hms.harvard.edu, Massachusetts General Hospital and Harvar
實驗概要Central neurons lose the ability for axonal regrowth during development and typically do not regenerate their axons following axotomy once the
AbstractWhen more than one bait will be used to screen a single library, significant time and resources can be saved by performing the interactor hunt
Lentivirus Transduction of Hematopoietic CellsMing-Jie Li and John J. RossiDivision of Molecular Biology, Beckman Research Institute of the City of Ho
MaterialsCytokine-specific Primary Antibodiesunlabeled or biotinylated antigen-affinity purified polyclonal antibodies (R&D Systems ''AF&#
The Inoue Method for Preparation and Transformation of Competent E. coli: "Ultra Competent" CellsJoseph SambrookPeter Maccallum Cancer Insti
實驗概要The PureLink? Genomic DNA Purification Kit allows rapid and efficient purification of genomic DNA. The kit is designed to efficiently
IntroductionThere are two modes of development common to most species in the animal kingdom.Virtually all embryos undergo both mosaic and regulative d
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In VitroRoxanne Holmes and Juan Carlos Zú?iga-Pfl
實驗概要 This protocol utilizes the powerful guanidine isothiocyanate–phenol:chloroform extraction method which allows the
Outline:Bacteriophage lambda is a linear double stranded DNA, approximately 50 kB in size. The two sticky ends help the phage to recircularize after e
This method gives yeast nuclei which look nearly purified microscopically. Nuclei isolated in this way do not give active transcription extracts when
CD7Membrane glycoprotein and Fc receptor for IgM Homologous to TCR gamma, Ig kappaMembrane expression early during T ontogeny, before TCR rearran