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The method we use is based on work of Dr. Hazel Cheng, at the University of Toronto and works for both colon and the small intestine.First we excise the whole colon from cecum to rectum. This is easily done after openning the abdomen by grasping the cecum with forceps and pulling until it is straight. Then cut close to the cecum and the rectum.2. Flush the colon using a 10 ml syringe and a large needle, preferably wi......閱讀全文
Intestinal intraepithelial lymphocytes Intestinal intraepithelial lymphocytes (IELs) are mostly T cells, which are continuously
CD7Membrane glycoprotein and Fc receptor for IgM Homologous to TCR gamma, Ig kappaMembrane expression early during T ontogeny, before TCR rearran
Coating of the Culture Dish SurfacesCulture dishes were incubated with a film of a 1:1 mixture of laminin (10 μg/ml and chondroitin sulfate (10 μg
ContentsEmbryonic Stem Cell MarkersHematopoietic Stem Cell MarkersMesenchymal/Stromal Stem Cell MarkersNeural Stem Cell MarkersReferencesWhile stem ce
7. Resuspend the pellet with 3 ml S1 solution (Figure 3). The pellet should be resuspended readily by pipetting up and down. A pellet that cannot be r
Thyroid epithelial cells (also called follicular cells or principal cells) are cells of thyroid gland that are responsible for the production and
恩他卡朋（Entacapone）是一種廣泛用于帕金森疾病（PD）治療的藥物，是兒茶酚-O-甲基轉移酶（COMT）的抑制劑。然而，恩他卡朋有胃腸副作用，臨床上約10%的服用者會出現腹瀉。為了研究產生腹瀉的機理，科學家采用的新方法非損傷微測技術結合傳統方法研究了恩他卡朋造成腹瀉的原因。2011年，首都醫
Isolation of Epithelial Cells from Rabbit Bladders 1. Animal experiments were performed in accordance with the Animal Use and Care Committee
Corneal keratocytes (corneal fibroblasts) that this corneal layer is specialized fibroblasts residing in the stroma, representing about 85-90% of
Tissue collection1. Endometrial biopsies were collected from women undergoing gynaecological procedures for benign conditions. 2. All wome
1.Human colonic epithelial cells (EC) were isolated from freshly resected colonic surgical specimens obtained. 2.All diagnoses were confirmed by clini
利用人誘導性多能干細胞產生肺細胞來自美國波士頓大學再生醫學中心（Center for Regenerative Medicine, CReM）的研究人員宣布了他們取得兩項重大的發現。他們希望這些結果將會導致人們開發出新的“個人化療法”來治療肺部疾病。培養和純化由人干細胞產生的最早肺祖細胞（一種未成熟
DNA抽提（主要內容如下）· Working with DNA· DNA Extraction from Bacteria and Other Organisms· DNA Extraction f
RNA提取（主要內容如下）Tips for Handing RNA Total RNA IsolationmRNA IsolationrRNA IsolationOthersQ & A posted in the Method Forum Basic Proc
實驗概要DNAzol? Reagent (Genomic DNA Isolation Reagent) is a complete and ready-to-use reagent for the isolation of genomic DNA from solid and
B. Midiprep double-stranded DNA isolationA midi-prep double-stranded DNA isolation has been developed to generate a sufficient amount of template DNA
DNA電泳（主要內容如下） Preparation of Agarose Gel and Electrophoresis Extraction of DNA From Agarose Gel Extraction of DNA fro
DNA Extraction· DNA Extraction from Bacteria (Julie B. Wolf,UMBC)Phenol/chloroform method·&n
實驗概要Human embryonic stem cells can be differentiated into neural-, mesenchymal and hematopoetic stem cells. This product is intended for t
Recent Advancements in mmWave Isolator TechnologyMarch 13, 2019 David W. Porterfield, Jr., Micro Harmonics Corp., Fincastle, Va. No Comments
實驗概要Interest in RNA-protein interactions is booming as we begin to appreciate the role of RNA, not just in well-established processes such as tran
DNA Isolation by a Cleared Lysate Method Followed by Double Acetate Precipitation Version 3b - updated September 26, 1999The Most Recent Roe Lab Imple
實驗概要Plant DNAzol? is an extra-strength-DNAzol? reagent (patent pending) specifically formulated for the isolation of genomic DNA from plants. The Plan
DNA PreparationGene TransferEmbryo TransferTransgenic IdentificatioinOthersTransgenic Outline (University of Michigan Transgenic Animal Model Cor
This procedure describes a convenient method for the isolation of crude nuclear pellets from N. crassa. The method, an adaptation of the one deve
Adrenal chromaffin granule (chromaffin vesicle) preparationIntroduction. This prep is adapted from the classic paper of Smith and Winkler (Smith
Western 雜交（主要內容如下）Preparing of Protein LysatesWestern BlottingFar Western BlottingSemi Dry BlottingStripping MembranesTrouble Shooting and Others
實驗概要 This protocol utilizes the powerful guanidine isothiocyanate–phenol:chloroform extraction method which allows the
蛋白質提取和純化（主要內容如下）Protein Extraction Protein PurificationProtein PrecipitationColumn PreparatioinQ & A Posted in the Method ForumProtein Extrac