WIKI資訊
PurposeTransient transfection into 293T cells is a convenient way to overexpress and obtain both cellular and extracellular (secreted or membrane) proteins. 293 is a human renal epithelial cell line which is transformed by adenovirus E1A gene product. 293T is a derivative which also express SV40 large T antigen, allowing episomal replication of plasmids containing the SV40 origin and early promoter region. They (both......閱讀全文
實驗概要Adenoviral vectors widely used to transfer foreign genes into neuronal cells possess tropism for glial cells and are toxic to infected cells.
Lentivirus Transduction of Hematopoietic CellsMing-Jie Li and John J. RossiDivision of Molecular Biology, Beckman Research Institute of the City of Ho
Generating stable cell lines in HEK293Prior to transfection, it is recommended that you linearize your pcDNA gene construct. Linearizing will decrease
Generating stable cell lines in HEK293Prior to transfection, it is recommended that you linearize your pcDNA gene construct. Linearizing will decrease
細胞系 Cell linesThe following CD19-expressing immortalized cell lines were used: Raji (Burkitt’s lymphoma cell line, ATCC-CCL86),Daudi (B lymphobla
ProcedureI. INTRODUCTIONThe 293 EBNA cell line is established from primary embryonal human kidney cells transformed with sheared human adenovirus type
Transient Transfection of Cos-1 CellsNote: This protocol has been optimized for Cos-1 cells. Successful transfection of each cell type requires o
Hematopoietic Stem Cell Targeting with Surface-Engineered Lentiviral VectorsEls Verhoeyen and Fran?ois-Lo?c CossetAdapted from Gene Tra
Application: Quantitative RT-PCR is used to quantify mRNA in both relative and absolute terms. It can be applied for the quantification of m
ASTRACTWe describe a rapid and quantitative flow cytometric method for determining the apoptotic or anti-apoptotic potential of a gene in various cell
Scanning the human genome with combinatorial transcription factor librariesPublished online: 18 February 2003, doi:10.1038/nbt794March 2003 Volume 21
人胚腎 293 (HEK293) 細胞在重組蛋白表達中是最常見的宿主細胞。 這類細胞能夠表達大量的膜蛋白，如 G 蛋白偶聯受體 (GPCR) ，是無法在最常見的生物制藥生產宿主，如：中國倉鼠卵巢 (CHO) 細胞中作表達。 HEK293 雖然是蛋白表達的極好宿主，然而 H
To assay 17 b-HSD activity in lysates, cells were harvested 48h after transfection using PBS Enzyme Free Cell Dissociation Solution ( specialty Media
Mammalian RNA InterferenceThomas TuschlLaboratory for RNA Molecular BiologyThe Rockefeller University, New York Excerpted from RNAi: A Guide
DNA轉染· Transfection of Mammalian Cells Using Lipofectamine (LTI)· &n
實驗概要Lipofectamine? LTX Reagent is a proprietary, animal-origin free formulation for the transfection of DNA into eukaryotic cells with low
Purpose and BackgroundsCHO lec 3.2.8.1 cellsCHO Lec 3.2.8.1 cells have four independent mutations in the N- and O- glycosylation pathways (Stanley, 19
Lysate preparation and western blottingProtein lysates were created by harvesting the cells from con?uent T-?asks or from suspension cultures at h
實驗概要293fectin? is a proprietary, cationic lipid-based formulation for transfection of DNA into eukaryotic cells. 293fectin? is optimized f
Figure 2. Silencer ? siRNA Validation Data Generated Using Applied Biosystems TaqMan? Gene Expression Assays. The indicated Silencer Validated siRNAs
Green fluorescent protein (GFP) is responsible for the bioluminescence of the Pacific Northwest jellyfish, Aequorea victoria. In A.victoria, the 27-kD
Metabolic biotinylation of mammalian cell receptors for imagingBakhos A. Tannous , btannous@hms.harvard.edu, Massachusetts General Hospital and Harvar
Restriction Map and Cloning Site of the RNAi-Ready pSIREN-DNR Vector. Unique restriction sites are in bold. RNAi-Ready pSIREN-DNR is provided as
實驗概要將轉移基因整合到細胞染色體DNA上,形成穩定表達轉移基因的細胞系。 實驗原理 細胞轉染技術是目前廣泛應用于病毒基因結構與功能以及基因調控等的研究。細胞轉染可分為短暫轉染和穩定(或永久) 轉染兩種。在短暫轉染中,被轉染基因并不整合至細胞染色體中,因而不能隨細
Cellular Transfection and Immunoprecipitation Before proceeding with the experiments outlined below, all kinase pocket mutants should be ch
TroubleshootingCritical Steps(1) Don’t trypsinize cells for too long when collecting them.(2) Rotation speed should be no more than 1500 rpm during ce
實驗概要Use PEI (Linear 25 kDa Reagent) to transfect in HEK293T cells.主要試劑PEI is polyethyleimine, a 25 kDa linear from Polysciences Inc. Make up solutio
The protocols listed here are for Drosophila cells in 6 well plates and our pre-aliquoted 384 well plates. RNAi experiments may be done in other size
越來越多的研究人員開始采用小分子干擾RNA（small interfering RNAs，siRNAs)來抑制特定的哺乳動物基因表達。siRNA是一種短片斷雙鏈RNA分子，能夠以同源互補序列的mRNA為靶目標降解特定的mRNA，這個過程就是RNA干擾途徑（RNA interf
Electroporation ProtocolPreparation of Electro-competent Cells:1. Grow XL1-Blue cells on a tetracycline plate (20 ug tet/ml of LB agar)2. Inoculate 3